Fermented Acanthopanax koreanum Root Extract Reduces UVB- and H2O2-Induced Senescence in Human Skin Fibroblast Cells
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초록
The present study assessed the effects of an aqueous extract of Acanthopanax koreanum root (AE) and of AE following fermentation by lactic acid bacteria (Lactobacillus plantarum and Bifidobacterium bifidum) (AEF) on human skin fibroblast HS68 cells exposed to ultraviolet B (UVB) irradiation and oxidative stress. AEF effectively antagonized the senescence-associated β-galactosidase staining and upregulation of p53 and p21Cip1/WAF1 induced by UVB or H2O2 treatment in HS68 cells. It also exhibited excellent antioxidant activities in radical scavenging assays and reduced the intracellular level of reactive oxygen species induced by UVB or H2O2 treatment. The antioxidant and antisenescent activities of AEF were greater than those of nonfermented A. koreanum extract. AEF significantly repressed the UVB- or H2O2-induced activities of matrix metalloproteinase (MMP)-1 and -3, overexpression of MMP-1, and nuclear factor κB (NF-κB) activation. This repression of NF-κB activation and MMP-1 overexpression was attenuated by a mitogen-activated protein kinase activator, suggesting that this AEF activity was dependent on this signaling pathway. Taken together, these data indicated that AEF-mediated antioxidant and anti-photoaging activities may produce anti-wrinkle effects on human skin.
Abstract
The present study assessed the effects of an aqueous extract of Acanthopanax koreanum root (AE) and of AE following fermentation by lactic acid bacteria (Lactobacillus plantarum and Bifidobacterium bifidum) (AEF) on human skin fibroblast HS68 cells exposed to ultraviolet B (UVB) irradiation and oxidative stress. AEF effectively antagonized the senescence-associated β-galactosidase staining and upregulation of p53 and p21Cip1/WAF1 induced by UVB or H2O2 treatment in HS68 cells. It also exhibited excellent antioxidant activities in radical scavenging assays and reduced the intracellular level of reactive oxygen species induced by UVB or H2O2 treatment. The antioxidant and antisenescent activities of AEF were greater than those of nonfermented A. koreanum extract. AEF significantly repressed the UVB- or H2O2-induced activities of matrix metalloproteinase (MMP)-1 and -3, overexpression of MMP-1, and nuclear factor κB (NF-κB) activation. This repression of NF-κB activation and MMP-1 overexpression was attenuated by a mitogen-activated protein kinase activator, suggesting that this AEF activity was dependent on this signaling pathway. Taken together, these data indicated that AEF-mediated antioxidant and anti-photoaging activities may produce anti-wrinkle effects on human skin.
참고문헌 (32)
- Akazaki S, "Age-related changes in skin wrinkles assessed by a novel three-dimensional morphometric analysis", Br. J. Dermatol, 147, pp.689~695 (2002)
- Al-Mohanna MA, "p53 is dispensable for UV-induced cell cycle arrest at late G1 in mammalian cells", Carcinogenesis, 22, pp.573~578 (2001)
- Bell S, "Involvement of NF-κB signalling in skin physiology and disease", Cell. Signal, 15, pp.1~7 (2003)
- Bickers DR, "Oxidative stress in the pathogenesis of skin disease", J. Invest. Dermatol, 126, pp.2565~2575 (2006)
- Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Bond M, "Nuclear factor k appaB activity is essential for matrix metalloproteinase-1 and -3 upregulation in rabbit dermal fibroblasts", Biochem. Biophys. Res. Commun, 264, pp.561~567 (1999)
- Cadet J, "DNA base damage by reactive oxygen species, oxidizing agents, and UV radiation", Cold Spring Harb. Perspect. Biol, 5, pp.a012559~ (2013)
- Cai XF, "Triterpenoids from Acanthopanax koreanum root and their inhibitory activities on NFAT transcription", Arch. Pharm. Res, 27, pp.825~828 (2004)
- Chen L, "The role of antioxidants in photoprotection: a critical review", J. Am. Acad. Dermatol, 67, pp.1013~1024 (2012)
- Di Girolamo N, "UVBelicited induction of MMP-1 expression in human ocular surface epithelial cells is mediated through the ERK1", 2 MAPK-dependent pathway, 2003, pp.44, 4705~4714
- Di Marzio L, "Increase of skin-ceramide levels in aged subjects following a short-term topical application of bacterial sphingomyelinase from Streptococcus thermophilus", Int. J. Immunopathol. Pharmacol, 21, pp.137~143 (2008)
- Farage MA, "Intrinsic and extrinsic factors in skin ageing: a review", Int. J. Cosmet. Sci, 30, pp.87~95 (2008)
- Fiore R, "Mycobacterium mucogenicum infection following a cosmetic procedure with poly- L -lactic acid", J. Drugs Dermatol, 12, pp.353~357 (2013)
- Fisher GJ, "Molecular basis of sun-induced premature skin ageing and retinoid antagonism", Nature, 379, pp.335~339 (1996)
- Goldstein S, "Replicative senescence: the human fibroblast comes of age", Science, 249, pp.1129~1133 (1990)
- Hahn DR, "A study on the chemical constituents of Acanthopanax koreanum Nakai and its pharmacobiological activities", Yakhak Hoeji, 29, pp.357~361 (1985)
- Jenkins G, "Molecular mechanisms of skin ageing", Mech. Ageing Dev, 123, pp.801~810 (2002)
- Jeon SM, "NADPH oxidase is involved in protein kinase CKII down-regulationmediated senescence through elevation of the level of reactive oxygen species in human colon cancer cells", FEBS Lett, 584, pp.3137~3142 (2010)
- Lewis DA, "UVB-induced activation of NF-kB is regulated by the IGF-1R and dependent on p38 MAPK", J. Invest. Dermatol, 128, pp.1022~1029 (2008)
- Lin QY, "Inhibition of inducible nitric oxide synthase by Acanthopnax senticosus extract in RAW264.7 macrophages", J. Ethnopharmacol, 118, pp.231~236 (2008)
- Liou GY, "Reactive oxygen species in cancer", Free Radic. Res, 44, pp.479~496 (2010)
- Nhiem NX, "Phenylpropanoids from the leaves of Acanthopanax koreanum and their antioxidant activity", J. Asian Nat. Prod. Res, 13, pp.56~61 (2011)
- Poljšak B, "The protective role of antioxidants in the defence against ROS", RNS-mediated environmental pollution, 2014, pp.2014, 671539~
- Poon RY, "Cyclindependent kinases are inactivated by a combination of p21 and Thr-14", Tyr-15 phosphorylation after UV-induced DNA damage, 1996, pp.271, 13283~13291
- Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radic. Biol. Med, 2, pp.1231~1237 (1999)
- Rittié L, "UV-light-induced signal cascades and skin aging", Ageing Res. Rev, 1, pp.705~720 (2002)
- Takema Y, "The relationship between age-related changes in the physical properties and development of wrinkles in human facial skin", J. Soc. Cosmet. Chem, 46, pp.163~173 (1995)
- Tsatsou F, "Extrinsic aging: UV-mediated skin carcinogenesis", Dermatoendocrinol, 4, pp.285~297 (2012)
- Watson RE, "Damage to skin extracellular matrix induced by UV exposure", Antioxid. Redox Signal, 21, pp.1063~1077 (2014)
- Wu YL, "Acanthoic acid, a diterpene in Acanthopanax koreanum, protects acetaminophen-induced hepatic toxicity in mice", Phytomedicine, 17, pp.475~479 (2010)
- Yang EJ, "Acanthopanax koreanum fruit waste inhibits lipopolysaccharideinduced production of nitric oxide and prostaglandin E2 in RAW 264.7 macrophages", J. Biomed. Biotechnol, 2010, pp.715739~ (2010)
- 양희선, "유산균 발효에 의한 버섯 추출물의 항산화 활성", 한국식품영양과학회지, 43(1), pp.80~85 (2014), 한국식품영양과학회