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Potential of Immobilized Whole-Cell Methylocella tundrae as a Biocatalyst for Methanol Production from Methane

김인원|이정걸|Primata Mardina|Jinglin Li|Sanjay K.S. Patel|Chandrabose Selvaraj
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2016|Journal of Microbiology and Biotechnology|제26권 제7호|pp. 1234-1241|DOI 10.4014/jmb.1602.02074

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methanebiocatalystimmobilizationmethanolwhole-cell immobilizationMethylocella tundrae
주제분류생물학

초록

Methanol is a versatile compound that can be biologically synthesized from methane (CH4) by methanotrophs using a low energy-consuming and environment-friendly process. Methylocella tundrae is a type II methanotroph that can utilize CH4 as a carbon and energy source. Methanol is produced in the first step of the metabolic pathway of methanotrophs and is further oxidized into formaldehyde. Several parameters must be optimized to achieve high methanol production. In this study, we optimized the production conditions and process parameters for methanol production. The optimum incubation time, substrate, pH, agitation rate, temperature, phosphate buffer and sodium formate concentration, and cell concentration were determined to be 24 h, 50% CH4, pH 7, 150 rpm, 30°C, 100 mM and 50 mM, and 18 mg/ml, respectively. The optimization of these parameters significantly improved methanol production from 0.66 to 5.18 mM. The use of alginate-encapsulated cells resulted in enhanced methanol production stability and reusability of cells after five cycles of reuse under batch culture conditions.

Abstract

Methanol is a versatile compound that can be biologically synthesized from methane (CH4) by methanotrophs using a low energy-consuming and environment-friendly process. Methylocella tundrae is a type II methanotroph that can utilize CH4 as a carbon and energy source. Methanol is produced in the first step of the metabolic pathway of methanotrophs and is further oxidized into formaldehyde. Several parameters must be optimized to achieve high methanol production. In this study, we optimized the production conditions and process parameters for methanol production. The optimum incubation time, substrate, pH, agitation rate, temperature, phosphate buffer and sodium formate concentration, and cell concentration were determined to be 24 h, 50% CH4, pH 7, 150 rpm, 30°C, 100 mM and 50 mM, and 18 mg/ml, respectively. The optimization of these parameters significantly improved methanol production from 0.66 to 5.18 mM. The use of alginate-encapsulated cells resulted in enhanced methanol production stability and reusability of cells after five cycles of reuse under batch culture conditions.

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관련 논문AI추천

Production of Methanol from Methane by Encapsulated Methylosinus sporiumBharat Madan, In-Won Kim, Jae-Hoon Jeong, Jung-Kul Lee, Jung Rim Haw, Liaoyuan Zhang, Sachin V. Otari, Sanjay K. S. Patel, Sanjeet Mehariya한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2016 · 제26권 제12호Biological Methanol Production by a Type II Methanotroph Methylocystis bryophila이정걸, In-Won Kim, Primata Mardina, Sang-Yong Kim, Sanjay K.S. Patel한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2016 · 제26권 제4호Methanotrophs을 이용한 메탄 저감 기술 최신 동향정혜경, 조경숙한국미생물·생명공학회 · 한국미생물·생명공학회지2017 · 제45권 제3호Adverse Effect of the Methanotroph Methylocystis sp. M6 on the Non-Methylotroph Microbacterium sp. NM2김태관, 정소연, 조경숙한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2018 · 제28권 제10호Insights into Enzyme Reactions with Redox Cofactors in Biological Conversion of CO2Kang Du-Kyeong, Kim Seung-Hwa, Sohn Jung-Hoon, Sung Bong Hyun한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2023 · 제33권 제11호Application of bioelectromethanation using an electroactive methanogen for the biogas upgradingByoung-In Sang, Jaesung Chun, Ji Sun Lee, Okkyoung Choi청정에너지연구소 · Journal of Ceramic Processing Research2020 · 제21권 제5호

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