본문 바로가기
KERD
논문KCI 등재

Biological Methanol Production by a Type II Methanotroph Methylocystis bryophila

이정걸|In-Won Kim|Primata Mardina|Sanjay K.S. Patel|Sang-Yong Kim
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2016|Journal of Microbiology and Biotechnology|제26권 제4호|pp. 717-724|DOI 10.4014/jmb.1601.01013

2

조회

0

다운로드

9

인용

0

참고문헌

키워드

methaneBiogasgreenhouse gasesmethanol productionMethylocystis bryophila
주제분류생물학

초록

Methane (CH4) is the most abundant component in natural gas. To reduce its harmful environmental effect as a greenhouse gas, CH4 can be utilized as a low-cost feed for the synthesis of methanol by methanotrophs. In this study, several methanotrophs were examined for their ability to produce methanol from CH4; including Methylocella silvestris, Methylocystis bryophila, Methyloferula stellata, and Methylomonas methanica. Among these methanotrophs, M. bryophila exhibited the highest methanol production. The optimum process parameters aided in significant enhancement of methanol production up to 4.63 mM. Maximum methanol production was observed at pH 6.8, 30°C, 175 rpm, 100 mM phosphate buffer, 50 mM MgCl2 as a methanol dehydrogenase inhibitor, 50% CH4 concentration, 24 h of incubation, and 9 mg of dry cell mass ml-1 inoculum load, respectively. Optimization of the process parameters, screening of methanol dehydrogenase inhibitors, and supplementation with formate resulted in significant improvements in methanol production using M. bryophila. This report suggests, for the first time, the potential of using M. bryophila for industrial methanol production from CH4.

Abstract

Methane (CH4) is the most abundant component in natural gas. To reduce its harmful environmental effect as a greenhouse gas, CH4 can be utilized as a low-cost feed for the synthesis of methanol by methanotrophs. In this study, several methanotrophs were examined for their ability to produce methanol from CH4; including Methylocella silvestris, Methylocystis bryophila, Methyloferula stellata, and Methylomonas methanica. Among these methanotrophs, M. bryophila exhibited the highest methanol production. The optimum process parameters aided in significant enhancement of methanol production up to 4.63 mM. Maximum methanol production was observed at pH 6.8, 30°C, 175 rpm, 100 mM phosphate buffer, 50 mM MgCl2 as a methanol dehydrogenase inhibitor, 50% CH4 concentration, 24 h of incubation, and 9 mg of dry cell mass ml-1 inoculum load, respectively. Optimization of the process parameters, screening of methanol dehydrogenase inhibitors, and supplementation with formate resulted in significant improvements in methanol production using M. bryophila. This report suggests, for the first time, the potential of using M. bryophila for industrial methanol production from CH4.

참고문헌 (43)

  1. Zi-Fang Chi, "Spatial Patterns of Methane Oxidation and Methanotrophic Diversity in Landfill Cover Soils of Southern China", Journal of Microbiology and Biotechnology, 25(4), pp.423~430 (2015), 한국미생물·생명공학회[DOI]
  2. Dhiman SS, "Simultaneous pretreatment and saccharification:green technology for enhanced sugar yields from biomass using a fungal consortium", Bioresour. Technol, 179, pp.50~57 (2015)
  3. Duan C, "High-rate conversion of methane to methanol by Methylosinus trichosporium OB3b", Bioresour. Technol, 102, pp.7349~7353 (2011)
  4. Fei Q, "Bioconversion of natural gas to liquid fuel: opportunities and challenges", Biotechnol. Adv, 32, pp.596~614 (2014)
  5. Gao H, "Repeated production of L-xylulose by an immobilized wholecell biocatalyst harboring L-arabinitol dehydrogenase coupled with an NAD+ regeneration system", Biochem. Eng. J, 96, pp.23~28 (2015)
  6. Ge X, "Biological conversion of methane to liquid fuels: status and opportunities", Biotechnol. Adv, 32, pp.1460~1475 (2014)
  7. Han J-S, "Partial oxidative conversion of methane to methanol through selective inhibition of methanol dehydrogenase in methanotrophic consortium from landfill cover soil", Appl. Biochem. Biotechnol, 171, pp.1487~1499 (2013)
  8. 황인엽, "Batch Conversion of Methane to Methanol Using Methylosinus trichosporium OB3b as Biocatalyst", Journal of Microbiology and Biotechnology, 25(3), pp.375~380 (2015), 한국미생물·생명공학회[DOI]
  9. 황인엽, "Biocatalytic Conversion of Methane to Methanol as a Key Step for Development of Methane-Based Biorefineries", Journal of Microbiology and Biotechnology, 24(12), pp.1597~1605 (2014), 한국미생물·생명공학회[DOI]
  10. Jamil M, "Renewable energy technologies adopted by the UAE: prospects and challenges - A comprehensive overview", Renew. Sustain. Energy Rev, 55, pp.1181~1194 (2016)
  11. Jung S-J, "Comparison of lignin, cellulose, and hemicellulose contents for biofuels utilization among 4 types of lignocellulosic crops", Biomass Bioenergy, 83, pp.322~327 (2015)
  12. Kalyani D, "Microbial consortia for saccharification of woody biomass and ethanol fermentation", Fuel, 107, pp.815~822 (2013)
  13. Kalyani D, "A highly efficient recombinant laccase from the yeast Yarrowia lipolytica and its application in the hydrolysis of biomass", PLoS One, 10, pp.e0120156~ (2015)
  14. Kalyuzhnaya MG, "Characterization of a novel methanol dehydrogenase in representatives of Burkholderiales: implications for environmental detection of methylotrophy and evidence for convergent evolution", J. Bacteriol, 190, pp.3817~3823 (2008)
  15. 김현중, "Optimization of Direct Lysine Decarboxylase Biotransformation for Cadaverine Production with Whole-Cell Biocatalysts at High Lysine Concentration", Journal of Microbiology and Biotechnology, 25(7), pp.1108~1113 (2015), 한국미생물·생명공학회[DOI]
  16. 김태수, "Reduction of Acetate and Lactate Contributed to Enhancement of a Recombinant Protein Production in E. coli BL21", Journal of Microbiology and Biotechnology, 25(7), pp.1093~1100 (2015), 한국미생물·생명공학회[DOI]
  17. Kumar P, "Extending the limits of Bacillus for novel biotechnological applications", Biotechnol. Adv, 31, pp.1543~1561 (2013)
  18. Kumar P, "Dark fermentative bioconversion of glycerol to hydrogen by Bacillus thuringiensis", Bioresour. Technol, 182, pp.383~388 (2015)
  19. Lee SG, "Optimization of methanol biosynthesis from methane using Methylosinus trichosporium OB3b", Biotechnol. Lett, 26, pp.947~950 (2004)
  20. 이상현, "A New Shuttle Plasmid That Stably Replicates in Clostridium acetobutylicum", Journal of Microbiology and Biotechnology, 25(10), pp.1702~1708 (2015), 한국미생물·생명공학회[DOI]
  21. Mehta PK, "Methanol biosynthesis by covalently immobilized cells of Methylosinus trichosporium:batch and continuous studies", Biotechnol. Bioeng, 37, pp.551~556 (1991)
  22. Mountfort DO, "Metal ionmediated accumulation of alcohols during alkane oxidation by whole cells of Methylosinus trichosporium", Enzyme Microb. Technol, 12, pp.343~348 (1990)
  23. Murrell JC, "The microbial methane cycle", Environ. Microbiol. Rep, 1, pp.279~284 (2009)
  24. 박동현, "Biological conversion of methane to methanol", Korean Journal of Chemical Engineering, 30(5), pp.977~987 (2013), 한국화학공학회[DOI]
  25. Sanjay K.S. Patel, "Immobilization of Laccase on SiO2 Nanocarriers Improves Its Stability and Reusability", Journal of Microbiology and Biotechnology, 24(5), pp.639~647 (2014), 한국미생물·생명공학회[DOI]
  26. Patel SKS, "Enhancement in hydrogen production by cocultures of Bacillus and Enterobacter", Int. J. Hydrogen Energy, 39, pp.14663~14668 (2014)
  27. Patel SKS, "Integrative approach to produce hydrogen and polyhydroxy alkanoate from biowaste using defined bacterial cultures", Bioresour. Technol, 176, pp.136~141 (2015)
  28. Pen N, "An innovative membrane bioreactor for methane biohydroxylation", Bioresour. Technol, 174, pp.42~52 (2014)
  29. Pierie F, "Environmental and energy system analysis of bio-methane production pathways: a comparison between feedstocks and process optimizations", Appl. Energy, 160, pp.456~466 (2015)
  30. 라채훈, "Detoxification of Eucheuma spinosum Hydrolysates with Activated Carbon for Ethanol Production by the Salt-Tolerant Yeast Candida tropicalis", Journal of Microbiology and Biotechnology, 25(6), pp.856~862 (2015), 한국미생물·생명공학회[DOI]
  31. Razumovsky SD, "Effect of immobilization on the main dynamic characteristics of the enzymatic oxidation of methane to methanol by bacteria Methylosinus sporium B-2121", Russ. Chem. Bull. Int. Ed, 57, pp.1633~1636 (2008)
  32. Maria Antonietta Ricci, "Improved 1,3-Propanediol Synthesis from Glycerol by the Robust Lactobacillus reuteri Strain DSM 20016", Journal of Microbiology and Biotechnology, 25(6), pp.893~902 (2015), 한국미생물·생명공학회[DOI]
  33. Senko O, "Methanol production in the flow system with immobilized cells Methylosinus sporium", XV International Workshop on Bioencapsulation, P2-16, pp.1~4 (2007)
  34. Shamsul NS, "An overview on the production of bio-methanol as potential renewable energy", Renew. Sustain. Energy Rev, 31, pp.578~588 (2014)
  35. Sheets JP, "Biological conversion of biogas to methanol using methanotrophs isolated from solid-state anaerobic digestate", Bioresour. Technol, 201, pp.50~57 (2016)
  36. Sigdel S, "Molecular dynamics simulation to rationalize regioselective hydroxylation of aromatic substrates by soluble methane monooxygenase", Bioorg. Med. Chem. Lett, 25, pp.1611~1615 (2015)
  37. Strong PJ, "Methane as a resource:can the methanotrophs add value", Environ. Sci. Technol, 49, pp.4001~4018 (2015)
  38. Takeguchi M, "Optimization of methanol biosynthesis by Methylosinus trichosporium OB3b: an approach to improve methanol accumulation", Appl. Biochem. Biotechnol, 68, pp.143~152 (1997)
  39. Trop P, "Production of methanol from a mixture of torrefied biomass and coal", Energy, 77, pp.125~132 (2014)
  40. Wood PJ, "Determination of methanol and its application to measurement of pectin ester content and pectin methyl esterase activity", Anal. Biochem, 39, pp.418~428 (1971)
  41. Xin J-Y, "Biosynthesis of methanol from CO2 and CH4 by methanotrophic bacteria", Biotechnology, 3, pp.67~71 (2004)
  42. Yoo Y-S, "Comparative enzyme inhibitive methanol production by Methylosinus sporium from simulated biogas", Environ. Technol, 36, pp.983~991 (2015)
  43. Chao Zhao, "Identification and Characterization of an Anaerobic Ethanol-Producing Cellulolytic Bacterial Consortium from Great Basin Hot Springs with Agricultural Residues and Energy Crops", Journal of Microbiology and Biotechnology, 24(9), pp.1280~1290 (2014), 한국미생물·생명공학회[DOI]

관련 논문AI추천

Potential of Immobilized Whole-Cell Methylocella tundrae as a Biocatalyst for Methanol Production from Methane김인원, 이정걸, Chandrabose Selvaraj, Jinglin Li, Primata Mardina, Sanjay K.S. Patel한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2016 · 제26권 제7호Production of Methanol from Methane by Encapsulated Methylosinus sporiumBharat Madan, In-Won Kim, Jae-Hoon Jeong, Jung-Kul Lee, Jung Rim Haw, Liaoyuan Zhang, Sachin V. Otari, Sanjay K. S. Patel, Sanjeet Mehariya한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2016 · 제26권 제12호Methanotrophs을 이용한 메탄 저감 기술 최신 동향정혜경, 조경숙한국미생물·생명공학회 · 한국미생물·생명공학회지2017 · 제45권 제3호Adverse Effect of the Methanotroph Methylocystis sp. M6 on the Non-Methylotroph Microbacterium sp. NM2김태관, 정소연, 조경숙한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2018 · 제28권 제10호Methane Oxidation Potentials of Rice-associated Plant Growth Promoting Methylobacterium Species월탱 덴버, 강연경, 사동민, 신완식, Sundaram Seshadri한국환경농학회 · 한국환경농학회지2022 · 제41권 제2호Characterization of Methanotrophic Communities in Soils from Regions with Different Environmental Settings김태관, 이상현, 조경숙, Hyun Jung Park, Kyung-Eun Moon, Pyeong-Wha Kim한국미생물·생명공학회 · 한국미생물·생명공학회지2012 · 제40권 제2호

이 간행물의 다른 논문 (6건)

Identification of Oligosaccharides in Human Milk Bound onto the Toxin A Carbohydrate Binding Site of Clostridium difficileJun Seong Park, Doman Kim, Tae-Su Jang, Kyeong Hwan Hwang, 김천형, Thi Thanh Hanh Nguyen, Jong Woon Kimpp. 659-665Comparative Analysis of the Complete Genome of Lactobacillus plantarum GB-LP2 and Potential Candidate Genes for Host Immune System Enhancement곽우리, DAE-KYUNG KANG, 조서애, Jaeyoung Heo, Heebal Kim, Sook Hee Yoon, Kyung Jin Cho, Jung-Sun Kang, Chanho Lee, Chul Lee, Kwondo Kimpp. 684-692Construction of an Efficient Mutant Strain of Trichosporonoides oedocephalis with HOG1 Gene Deletion for Production of ErythritolJingsheng Gu, Haiyang Zhang, Liangzhi Li, Tianyi Yang, Weiqiang Guo, Xin Ju, Cuiying Hu, Bingyu Tang, Jiaolong Fupp. 700-709Evaluation of Biogas Production Performance and Archaeal Microbial Dynamics of Corn Straw during Anaerobic Co-Digestion with Cattle Manure LiquidRenzhe Piao, Weidong Wang, Hongyan Zhao, Xue Li, Hairu Yu, Benyue Zhang, Zongjun Cui, Di Chenpp. 739-747Comparison of Anodic Community in Microbial Fuel Cells with Iron Oxide-Reducing CommunityTakahiro Yamashita, Mitsuyoshi Ishida, Hiroshi Yokoyamapp. 757-762
원문 보기