본문 바로가기
KERD
논문KCI 등재

Hed1 Promotes Meiotic Crossover Formation in Saccharomyces cerevisiae

1

조회

0

다운로드

3

인용

0

참고문헌

키워드

주제분류생물학

초록

Homologous recombination occurs between homologous chromosomes and is significantly involved in programmed double-strand break (DSB) repair. Activation of two recombinases, Rad51 and Dmc1, is essential for an interhomolog bias during meiosis. Rad51 participates in both mitotic and meiotic recombination, and its strand exchange activity is regulated by an inhibitory factor during meiosis. Thus, activities of Rad51 and Dmc1 are coordinated to promote homolog bias. It has been reported that Hed1, a meiosis-specific protein in budding yeast, regulates Rad51-dependent recombination activity. Here, we investigated the role of Hed1 in meiotic recombination by ectopic expression of the protein after pre-meiotic replication in Saccharomyces cerevisiae. DNA physical analysis revealed that the overexpression of Hed1 delays the DSB-to-joint molecule (JM) transition and promotes interhomolog JM formation. The study indicates a possible role of Hed1 in controlling the strand exchange activity of Rad51 and, eventually, meiotic crossover formation.

Abstract

Homologous recombination occurs between homologous chromosomes and is significantly involved in programmed double-strand break (DSB) repair. Activation of two recombinases, Rad51 and Dmc1, is essential for an interhomolog bias during meiosis. Rad51 participates in both mitotic and meiotic recombination, and its strand exchange activity is regulated by an inhibitory factor during meiosis. Thus, activities of Rad51 and Dmc1 are coordinated to promote homolog bias. It has been reported that Hed1, a meiosis-specific protein in budding yeast, regulates Rad51-dependent recombination activity. Here, we investigated the role of Hed1 in meiotic recombination by ectopic expression of the protein after pre-meiotic replication in Saccharomyces cerevisiae. DNA physical analysis revealed that the overexpression of Hed1 delays the DSB-to-joint molecule (JM) transition and promotes interhomolog JM formation. The study indicates a possible role of Hed1 in controlling the strand exchange activity of Rad51 and, eventually, meiotic crossover formation.

참고문헌 (37)

  1. Roeder GS., "Meiotic chromosomes: it takes two to tango", Genes Dev, 11, pp.2600~2621 (1997)
  2. Kleckner N, "Genome Organization and Function in the Cell Nucleus" (2011), WileyVCH
  3. Zickler D, "Meiotic chromosomes: integrating structure and function", Annu. Rev. Genet, 33, pp.603~754 (1999)
  4. Arora C, "Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism", Mol. Cell, 13, pp.549~559 (2004)
  5. Kee K, "Spatial organization and dynamics of the association of Rec102 and Rec104 with meiotic chromosomes", EMBO J, 23, pp.1815~1824 (2004)
  6. Keeney S., "Mechanism and control of meiotic recombination initiation", Curr. Top. Dev. Biol, 52, pp.1~53 (2001)
  7. Prieler S, "The control of Spo11’s interaction with meiotic recombination hotspots", Genes Dev, 19, pp.255~269 (2005)
  8. Paull TT, "The 3’ to 5’ exonuclease activity of Mre11 facilitates repair of DNA double-strand breaks", Mol. Cell, 1, pp.969~979
  9. Usui T, "Complex formation and functional versatility of Mre11 of budding yeast in recombination", Cell, 95, pp.705~716 (1998)
  10. Cannavo E, "Sae2 promotes dsDNA endonuclease activity within Mre11-Rad50-Xrs2 to resect DNA breaks", Nature, 514, pp.122~125 (2014)
  11. Gasior SL, "Rad52 associates with RPA and functions with Rad55and Rad57 to assemble meiotic recombination complexes", Genes Dev, 12, pp.2208~2221 (1998)
  12. Hays SL, "Complex formation in yeast double-strand break repair: participation of Rad51, Rad52, Rad55, and Rad57 proteins", Proc. Natl. Acad. Sci. USA, 92, pp.6925~6929 (1995)
  13. Sugiyama T, "A single-stranded DNA-binding protein is needed for efficient presynaptic complex formation by the Saccharomyces cerevisiae Rad51 protein", J. Biol. Chem, 272, pp.7940~7945 (1997)
  14. Sung P., "Function of yeast Rad52 protein as a mediator between replication protein A and the R ad51 recombinase", J. Biol. Chem, 272, pp.28194~28197 (1997)
  15. Hong EL, "Saccharomyces cerevisiae Dmc1 protein promotes renaturation of single-strand DNA (ssDNA) and assimilation of ssDNA into homologous super-coiled duplex DNA", J. Biol. Chem, 276, pp.41906~41912 (2001)
  16. Bishop DK, "RecA homologs Dmc1 and Rad51 interact to form multiple nuclear complexes prior to meiotic chromosome synapsis", Cell, 79, pp.1081~1092 (1994)
  17. Shinohara A, "Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein", Cell, 69, pp.457~470 (1992)
  18. Krejci L, "Homologous recombination and its regulation", Nucleic Acids Res, 40, pp.5795~5818 (2012)
  19. Hong S, "The logic and mechanism of homologous recombination partner choice", Mol. Cell, 51, pp.440~453 (2013)
  20. Jiang H, "Direct association between the yeast Rad51 and Rad54 recombination proteins", J. Biol. Chem, 271, pp.33181~33186 (1996)
  21. Sehorn MG, "Meiotic recombination: an affair of two recombinases", Cell Cycle, 3, pp.1375~1377 (2004)
  22. Sung P, "Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast Rad51 protein", Science, 265, pp.1241~1243 (1994)
  23. Bishop DK, "DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression", Cell, 69, pp.439~456 (1992)
  24. Bishop DK, "Early decision; meiotic crossover interference prior to stable strand exchange and synapsis", Cell, 117, pp.9~15 (2004)
  25. Sasanuma H, "A new protein complex promoting the assembly of Rad51 filaments", Nat. Commun, 4, pp.1676~ (2013)
  26. Busygina V, "Hed1 regulates Rad51-mediated recombination via a novel mechanism", Genes Dev, 22, pp.786~795 (2008)
  27. Tsubouchi H, "Budding yeast Hed1 downregulates the mitotic recombination machinery when meiotic recombination is impaired", Genes Dev, 20, pp.1766~1775 (2006)
  28. Janke C, "A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes", Yeast, 21, pp.947~962 (2004)
  29. 홍수길, "Shu1 Promotes Homolog Bias of Meiotic Recombination in Saccharomyces cerevisiae", Molecules and Cells, 36(5), pp.446~454 (2013), 한국분자세포생물학회
  30. 이민수, "Mitotic Cohesin Subunit Mcd1 Regulates the Progression of Meiotic Recombination in Budding Yeast", Journal of Microbiology and Biotechnology, 25(5), pp.598~605 (2015), 한국미생물·생명공학회[DOI]
  31. Kim KP, "Sister cohesion and structural axis components mediate homolog bias of meiotic recombination", Cell, 143, pp.924~937 (2010)
  32. Schwacha A, "Identification of joint molecules that form frequently between homologs but rarely between sister chromatids during yeast meiosis", Cell, 76, pp.51~63 (1994)
  33. 조홍래, "Hop2 and Sae3 Are Required for Dmc1-Mediated Double-Strand Break Repair via Homolog Bias during Meiosis", Molecules and Cells, 39(7), pp.550~556 (2016), 한국분자세포생물학회[DOI]
  34. 홍수길, "Ycs4 is Required for Efficient Double-Strand Break Formation and Homologous Recombination During Meiosis", Journal of Microbiology and Biotechnology, 25(7), pp.1026~1035 (2015), 한국미생물·생명공학회[DOI]
  35. Lee MS, "Functional validation of rare human genetic variants involved in homologous recombination using Saccharomyces cerevisiae", PLoS One, 10, pp.e0124152~ (2015)
  36. Yoon SW, "Meiotic prophase roles of Rec8 in crossover recombination and chromosome structure", Nucleic Acids Res, 44, pp.9296~9314 (2016)
  37. Lao JP, "Meiotic crossover control by concerted action of Rad51-Dmc1 in homolog template bias and robust homeostatic regulation", PLoS Genet, 9, pp.e1003978~ (2013)

관련 논문AI추천

이 간행물의 다른 논문 (13건)