Mec1 Modulates Interhomolog Crossover and Interplays with Tel1 at Post Double-Strand Break Stages
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
During meiosis I, programmed DNA double-strand breaks (DSBs) occur to promote chromosome pairing and recombination between homologs. In Saccharomyces cerevisiae, Mec1 and Tel1, the orthologs of human ATR and ATM, respectively, regulate events upstream of the cell cycle checkpoint to initiate DNA repair. Tel1ATM and Mec1ATR are required for phosphorylating various meiotic proteins during recombination. This study aimed to investigate the role of Tel1ATM and Mec1ATR in meiotic prophase via physical analysis of recombination. Tel1ATM cooperated with Mec1ATR to mediate DSB-to-single end invasion transition, but negatively regulated DSB formation. Furthermore, Mec1ATR was required for the formation of interhomolog joint molecules from early prophase, thus establishing a recombination partner choice. Moreover, Mec1ATR specifically promoted crossover-fated DSB repair. Together, these results suggest that Tel1ATM and Mec1ATR function redundantly or independently in all post-DSB stages.
Abstract
During meiosis I, programmed DNA double-strand breaks (DSBs) occur to promote chromosome pairing and recombination between homologs. In Saccharomyces cerevisiae, Mec1 and Tel1, the orthologs of human ATR and ATM, respectively, regulate events upstream of the cell cycle checkpoint to initiate DNA repair. Tel1ATM and Mec1ATR are required for phosphorylating various meiotic proteins during recombination. This study aimed to investigate the role of Tel1ATM and Mec1ATR in meiotic prophase via physical analysis of recombination. Tel1ATM cooperated with Mec1ATR to mediate DSB-to-single end invasion transition, but negatively regulated DSB formation. Furthermore, Mec1ATR was required for the formation of interhomolog joint molecules from early prophase, thus establishing a recombination partner choice. Moreover, Mec1ATR specifically promoted crossover-fated DSB repair. Together, these results suggest that Tel1ATM and Mec1ATR function redundantly or independently in all post-DSB stages.
참고문헌 (43)
- Kleckner N, "In genome organization and function in the cell nucleus, Chapter 19" (2011), Wiley-VCH
- Kim KP, "Sister cohesion and structural axis components mediate homolog bias of meiotic recombination", Cell, 143, pp.924~937 (2010)
- Sanchez-Moran E, "ASY1 mediates AtDMC1-dependent interhomolog recombination during meiosis in Arabidopsis", Genes Dev, 21, pp.2220~2233 (2007)
- Lao JP, "Trying to avoid your sister", PLoS Biol, 8, pp.e1000519~ (2010)
- Keeney S, "Meiosis-specific DNA double-strand breaks are catalyzed by Spo11, a member of a widely conserved protein family", Cell, 88, pp.375~384 (1997)
- Keeney S, "Mechanism and control of meiotic recombination initiation", Curr. Top. Dev. Biol, 52, pp.1~53 (2001)
- Neale MJ, "Endonucleolytic processing of covalent protein-linked DNA double-strand breaks", Nature, 436, pp.1053~1057 (2005)
- Murakami H, "Regulating the formation of DNA double-strand breaks in meiosis", Genes Dev, 22, pp.286~292 (2008)
- Cannavo E, "Sae2 promotes dsDNA endonuclease activity within Mre11-Rad50-Xrs2 to resect DNA breaks", Nature, 514, pp.122~125 (2014)
- Garcia V, "Bidirectional resection of DNA double-strand breaks by Mre11 and Exo1", Nature, 479, pp.241~244 (2011)
- Ribeiro J, "RPA homologs and ssDNA processing during meiotic recombination", Chromosoma, 125, pp.265~276 (2016)
- Sasanuma H, "A new protein complex promoting the assembly of Rad51 filaments", Nat. Commun, 4, pp.1676~ (2013)
- 홍수길, "Shu1 Promotes Homolog Bias of Meiotic Recombination in Saccharomyces cerevisiae", Molecules and Cells, 36(5), pp.446~454 (2013), 한국분자세포생물학회
- Borner GV, "Crossover", noncrossover differentiation, synaptonemal complex formation, and regulatory surveillance at the leptotene, Cell, pp.2004, 117[DOI]
- Grushcow JM, "Saccharomyces cerevisiae checkpoint genes MEC1, RAD17 and RAD24 are required for normal meiotic recombination partner choice", Genetics, 153, pp.607~620 (1999)
- Thompson D, "Genetic control of recombination partner preference in yeast meiosis : isolation and characterization of mutants elevated for meiotic unequal sister-chromatid recombination", Genetics, 153, pp.621~641 (1999)
- Carballo JA, "Phosphorylation of the axial element protein Hop1 by Mec1", Tel1 ensures meiotic interhomolog recombination, 2008, pp.132, 758~770
- Traven A, "SQ", TQ cluster domains : concentrated ATM, Bioessays, pp.2005, 27[DOI]
- Chuang CN, "Mek1 stabilizes Hop1-Thr318 phosphorylation to promote interhomolog recombination and checkpoint responses during yeast meiosis", Nucleic Acids Res, 40, pp.11416~11427 (2012)
- Navadgi-Patil VM, "A tale of two tails : activation of DNA damage checkpoint kinase Mec1", ATR by the 9-1-1 clamp and by Dpb11, DNA Repair(Amst), pp.2009, 8[DOI]
- Falck J, "Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage", Nature, 434, pp.605~611 (2005)
- Usui T, "A DNA damage response pathway controlled by Tel1 and the Mre11 complex", Mol. Cell, 7, pp.1255~1266 (2001)
- You Z, "ATM activation and its recruitment to damaged DNA require binding to the C terminus of Nbs1", Mol. Cell Biol, 25, pp.5363~5379 (2005)
- Melo J, "A unified view of the DNAdamage checkpoint", Curr. Opin. Cell Biol, 14, pp.237~245 (2002)
- 최호정, "Simultaneous and Sequential Integration by Cre", loxP Site-Specific Recombination in Saccharomyces cerevisiae, 2018, pp.28(5), 826~830[DOI]
- Hong-juan Jing, "Ethanol Induces Autophagy Regulated by Mitochondrial ROS in Saccharomyces cerevisiae", Journal of Microbiology and Biotechnology, 28(12), pp.1982~1991 (2018), 한국미생물·생명공학회
- Hunter N, "The single-end invasion : an asymmetric intermediate at the double-strand break to double-Holliday junction transition of meiotic recombination", Cell, 106, pp.59~70 (2001)
- Hong S, "The logic and mechanism of homologous recombination partner choice", Mol. Cell, 51, pp.440~453 (2013)
- 이민수, "Mitotic Cohesin Subunit Mcd1 Regulates the Progression of Meiotic Recombination in Budding Yeast", Journal of Microbiology and Biotechnology, 25(5), pp.598~605 (2015), 한국미생물·생명공학회[DOI]
- Lange J, "ATM controls meiotic double-strand-break formation", Nature, 479, pp.237~240 (2011)
- Garcia V, "Tel1(ATM)-mediated interference suppresses clustered meiotic double-strand-break formation", Nature, 520, pp.114~118 (2015)
- Zhang L, "Meiotic double-strand breaks occur once per pair of (sister) chromatids and, via Mec1", ATR and Tel1, Proc. Natl. Acad. Sci. USA, pp.2011, 108[DOI]
- Kato R, "An essential gene, ESR1, is required for mitotic cell growth, DNA repair and meiotic recombination in Saccharomyces cerevisiae", Nucleic Acids Res., 22, pp.3104~3012 (1994)
- Carr AM, "Control of cell cycle arrest by the Mec1sc", Rad3sp DNA structure checkpoint pathway, 1997, pp.7, 93~98
- Zhao X, "A suppressor of two essential checkpoint genes identifies a novel protein that negatively affects dNTP pools", Mol. Cell, 2, pp.329~340 (1998)
- Grenon M, "Double-strand breaks trigger MRX-and Mec1-dependent, but Tel1-independent, checkpoint activation", FEMS Yeast Res, 6, pp.836~847 (2006)
- Uziel T, "Requirement of the MRN complex for ATM activation by DNA damage", EMBO J, 22, pp.5612~5621 (2003)
- Mohibullah N, "Numerical and spatial patterning of yeast meiotic DNA breaks by Tel1", Genome Res, 27, pp.278~288 (2017)
- Beyer T, "Mec1 and Tel1 : an arresting dance of resection", EMBO J, 33, pp.176~178 (2014)
- Lee BH, "Role of Polo-like kinase CDC5 in programming meiosis I chromosome segregation", Science, 300, pp.482~486 (2003)
- Jin H, "Pds5 is required for homologue pairing and inhibits synapsis of sister chromatids during yeast meiosis", J. Cell Biol, 186, pp.713~725 (2009)
- Yoon SW, "Meiotic prophase roles of Rec8 in crossover recombination and chromosome structure", Nucleic Acids Res, 44, pp.9296~9314 (2016)
- 홍수길, "The nature of meiotic chromosome dynamics and recombination in budding yeast", The Journal of Microbiology, 57(4), pp.221~231 (2019), 한국미생물학회[DOI]
