Rapid Dot-Blot Immunoassay for Detecting Multiple Salmonella enterica Serotypes
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Salmonella, a major contributor to foodborne infections, typically causes self-limiting gastroenteritis. However, it is frequently invasive and disseminates across the intestinal epithelium, leading to deadly bacteremia. Although the genus is subdivided into >2,600 serotypes based on their antigenic determinants, only few serotypes are responsible for most human infections. In this study, a rapid dot-blot immunoassay was developed to diagnose multiple Salmonella enterica serotypes with high incidence rates in humans. The feasibility of 10 commercial antibodies (four polyclonal and six monoclonal antibodies) was tested using the 18 serotypes associated with 67.5% Salmonella infection cases in the United States of America (U.S.A) in 2016. Ab 3 (polyclonal; eight of 18 serotypes), Ab 8 (monoclonal; 13 of 18 serotypes), and Ab 9 (monoclonal; 10 of 18 serotypes) antibodies exhibited high detection rates in western blotting and combinations of two antibodies (Ab 3+8, Ab 3+9, and Ab 8+9) were applied to dot-blot assays. The combination of Ab 3+8 identified 15 of the tested 18 serotypes in 3 h, i.e., S. Enteritidis, S. Typhimurium, S. Javiana, S. I 4,[5],12:i:-, S. Infantis, S. Montevideo, S. Braenderup, S. Thompson, S. Saintpaul, S. Heidelberg, S. Oranienburg, S. Bareilly, S. Berta, S. Agona, and S. Anatum, which were responsible for 53.7% Salmonella infections in the U.S. in 2016. This cost-effective and rapid method can be utilized as an on-site colorimetric method for Salmonella detection.
Abstract
Salmonella, a major contributor to foodborne infections, typically causes self-limiting gastroenteritis. However, it is frequently invasive and disseminates across the intestinal epithelium, leading to deadly bacteremia. Although the genus is subdivided into >2,600 serotypes based on their antigenic determinants, only few serotypes are responsible for most human infections. In this study, a rapid dot-blot immunoassay was developed to diagnose multiple Salmonella enterica serotypes with high incidence rates in humans. The feasibility of 10 commercial antibodies (four polyclonal and six monoclonal antibodies) was tested using the 18 serotypes associated with 67.5% Salmonella infection cases in the United States of America (U.S.A) in 2016. Ab 3 (polyclonal; eight of 18 serotypes), Ab 8 (monoclonal; 13 of 18 serotypes), and Ab 9 (monoclonal; 10 of 18 serotypes) antibodies exhibited high detection rates in western blotting and combinations of two antibodies (Ab 3+8, Ab 3+9, and Ab 8+9) were applied to dot-blot assays. The combination of Ab 3+8 identified 15 of the tested 18 serotypes in 3 h, i.e., S. Enteritidis, S. Typhimurium, S. Javiana, S. I 4,[5],12:i:-, S. Infantis, S. Montevideo, S. Braenderup, S. Thompson, S. Saintpaul, S. Heidelberg, S. Oranienburg, S. Bareilly, S. Berta, S. Agona, and S. Anatum, which were responsible for 53.7% Salmonella infections in the U.S. in 2016. This cost-effective and rapid method can be utilized as an on-site colorimetric method for Salmonella detection.
참고문헌 (48)
- Crum-Cianflone NF, "Salmonellosis and the gastrointestinal tract : more than just peanut butter", Curr. Gastroenterol. Rep, 10, pp.424~431 (2008)
- Ehuwa O, "Salmonella, food safety and food handling practices", Foods, 10, pp.907~ (2021)
- Popoff MY, "Supplement 1998 (no. 42) to the Kauffmann-white scheme", Res. Microbiol, 151, pp.63~65 (2000)
- Brenner FW, "Salmonella nomenclature", J. Clin. Microbiol, 38, pp.2465~2467 (2000)
- Ferrari RG, "Worldwide epidemiology of Salmonella Serovars in animal-based foods : a meta-analysis", Appl. Environ. Microbiol, 85, pp.e00591-19~ (2019)
- Issenhuth-Jeanjean S, "Supplement 2008-2010 (no. 48) to the White-Kauffmann-Le Minor scheme", Res. Microbiol, 165, pp.526~530 (2014)
- Gal-Mor O, "Same species, different diseases : how and why typhoidal and non-typhoidal Salmonella enterica serovars differ", Front. Microbiol, 5, pp.391~ (2014)
- Zha L, "Salmonella infection in chronic inflammation and gastrointestinal cancer", Diseases, 7, pp.28~ (2019)
- Crump JA, "Epidemiology, clinical presentation, laboratory diagnosis, antimicrobial resistance, and antimicrobial management of invasive Salmonella Infections", Clin. Microbiol. Rev, 28, pp.901~937 (2015)
- "National Enteric Disease Surveillance: Salmonella Annual Report, 2016" (2018)[URL]
- Kipper D, "Emergence, dissemination and antimicrobial resistance of the main poultry-associated Salmonella serovars in Brazil", Vet. Sci, 9, pp.405~ (2022)
- Uelze L, "Toward an integrated genome-based Surveillance of Salmonella enterica in Germany", Front. Microbiol, 12, pp.626941~ (2021)
- Stott DI, "Immunoblotting and dot blotting", J. Immunol. Methods, 119, pp.153~187 (1989)
- Sarasombath S, "Characterization of monoclonal antibodies to protein antigen of Salmonella typhi", J. Clin. Microbiol, 26, pp.508~512 (1988)
- Jaradat ZW, "Optimization of a rapid dot-blot immunoassay for detection of Salmonella enterica serovar Enteritidis in poultry products and environmental samples", Food Microbiol, 21, pp.761~769 (2004)
- Park S, "Transcriptional insight into the effect of benzalkonium chloride on resistance and virulence potential in Salmonella Typhimurium", Microbiol. Res, 266, pp.127240~ (2023)
- van der Zee H, "Conventional methods for the detection and isolation of Salmonella enteritidis", Int. J. Food Microbiol, 21, pp.41~46 (1994)
- Awang MS, "Advancement in Salmonella detection methods : From conventional to electrochemical-based sensing detection", Biosensors(Basel), 11, pp.346~ (2021)
- Diep B, "Salmonella serotyping; Comparison of the traditional method to a microarray-based method and an in silico platform using whole genome sequencing data", Front. Microbiol, 10, pp.2554~ (2019)
- Zou QH, "Genotyping of Salmonella with lineage-specific genes : correlation with serotyping", Int. J. Infect. Dis, 49, pp.134~140 (2016)
- Yang SM, "Rapid real-time polymerase chain reaction for Salmonella serotyping based on novel unique gene markers by pangenome analysis", Front. Microbiol, 12, pp.750379~ (2021)
- Ferone M, "Microbial detection and identification methods : Bench top assays to omics approaches", Compr. Rev. Food Sci. Food Saf, 19, pp.3106~3129 (2020)
- Schenk F, "Development of a paper-based lateral flow immunoassay for simultaneous detection of lipopolysaccharides of Salmonella serovars", Anal. Bioanal. Chem, 410, pp.863~868 (2018)
- Wang B, "Immunoassay biosensing of foodborne pathogens with surface plasmon resonance imaging : A review", J. Agric. Food Chem, 68, pp.12927~12939 (2020)
- Huang H, "Evaluation of culture enrichment procedures for use with Salmonella detection immunoassay", Int. J. Food Microbiol, 51, pp.85~94 (1999)
- Lee HA, "Rapid enzyme-linked immunosorbent assays for the detection of Salmonella enteritidis in eggs", Food Agric. Immunol, 1, pp.89~99 (1989)
- Bruce VJ, "Evaluation of nanobody conjugates and protein fusions as bioanalytical reagents", Anal. Chem, 89, pp.3819~3823 (2017)
- Bayrac C, "DNA aptamer-based colorimetric detection platform for Salmonella Enteritidis", Biosens. Bioelectron, 98, pp.22~28 (2017)
- Silva NFD, "Electrochemical biosensors for Salmonella : State of the art and challenges in food safety assessment", Biosens. Bioelectron, 99, pp.667~682 (2018)
- Liu D, "Trends in miniaturized biosensors for point-of-care testing", TrAC Trends Anal. Chem, 122, pp.115701~ (2020)
- Farhana A, "Biochemistry, Lipopolysaccharide" (2003), StatPearls Publishing
- Bertani B, "Function and biogenesis of lipopolysaccharides", EcoSal Plus, 8, pp.10.1128~ (2018)
- Raetz CR, "Lipid A modification systems in gram-negative bacteria", Annu. Rev. Biochem, 76, pp.295~329 (2007)
- Raetz CR, "Lipopolysaccharide endotoxins", Annu. Rev. Biochem, 71, pp.635~700 (2002)
- Whitfield C, "Lipopolysaccharide O-antigens-bacterial glycans made to measure", J. Biol. Chem, 295, pp.10593~10609 (2020)
- Liu B, "Structural diversity in Salmonella O antigens and its genetic basis", FEMS Microbiol. Rev, 38, pp.56~89 (2014)
- Masi A, "Detection of live and heat-treated Salmonella enteritidis by a D1-serospecific anti-lipopolysaccharide O-9 monoclonal antibody", Food Agric. Immunol, 7, pp.351~363 (2008)
- Jinapon C, "Development of monoclonal antibodies for the rapid detection and identification of Salmonella enterica serovar Enteritidis in food sample using dot‐blot assays", J. Food Saf (2020)[DOI]
- Keller LH, "Monoclonal antibody-based detection system for Salmonella enteritidis", Avian Dis, 37, pp.501~507 (1993)
- Yoshimasu MA, "Application of rapid dot blot immunoassay for detection of Salmonella enterica serovar enteritidis in eggs, poultry, and other foods", Appl. Environ. Microbiol, 67, pp.459~461 (2001)
- Karberg KA, "Similarity of genes horizontally acquired by Escherichia coli and Salmonella enterica is evidence of a supraspecies pangenome", Proc. Natl. Acad. Sci. USA, 108, pp.20154~20159 (2011)
- Hu B, "Structural and genetic evidence for the close relationship between Escherichia coli O71 and Salmonella enterica O28 O-antigens", FEMS Immunol. Med. Microbiol, 59, pp.161~169 (2010)
- Miller T, "Epidemiological relationship between S. Infantis isolates of human and broiler origin", Lohmann. Info, 45, pp.27~31 (2010)
- Arnold K, "Using genetic markers for detection and subtyping of the emerging Salmonella enterica subspecies enterica serotype Muenchen", Poult. Sci, 101, pp.102181~ (2022)
- Lalsiamthara J, "Corrigendum : Pathogenic traits of Salmonella Montevideo in experimental infections in vivo and in vitro", Sci. Rep, 7, pp.46786~ (2017)
- Hoffmann M, "Tracing origins of the Salmonella bareilly strain causing a food-borne outbreak in the United States", J. Infect. Dis, 213, pp.502~508 (2016)
- "Differential Interactions of Lipopolysaccharides with Lipid Bilayers: Applications for Pathogen Detection" (2016)[URL]
- Sturm S, "Immunoblotting procedure for the analysis of electrophoretically-fractionated bacterial lipopolysaccharide", Arch. Microbiol, 140, pp.198~201 (1984)