Multilayer Coating with Red Ginseng Dietary Fiber Improves Intestinal Adhesion and Proliferation of Probiotics in Human Intestinal Epithelial Models
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
To exert their beneficial effects, it is essential for the commensal bacteria of probiotic supplements to be sufficiently protected as they pass through the low pH environment of the stomach, and effectively colonize the intestinal epithelium downstream. Here, we investigated the effect of a multilayer coating containing red ginseng dietary fiber, on the acid tolerance, and the adhesion and proliferation capacities of three Lactobacillus strains (Limosilactobacillus reuteri KGC1901, Lacticaseibacillus casei KGC1201, Limosilactobacillus fermentum KGC1601) isolated from Panax ginseng, using HT-29 cells, mucin-coated plates, and human pluripotent stem cell-derived intestinal epithelial cells as in vitro models of human gut physiology. We observed that the multilayer-coated strains displayed improved survival rates after passage through gastric juice, as well as high adhesion and proliferation capacities within the various gut epithelial systems tested, compared to their uncoated counterparts. Our findings demonstrated that the multilayer coat effectively protected commensal microbiota and led to improved adhesion and colonization of intestinal epithelial cells, and consequently to higher probiotic efficacy.
Abstract
To exert their beneficial effects, it is essential for the commensal bacteria of probiotic supplements to be sufficiently protected as they pass through the low pH environment of the stomach, and effectively colonize the intestinal epithelium downstream. Here, we investigated the effect of a multilayer coating containing red ginseng dietary fiber, on the acid tolerance, and the adhesion and proliferation capacities of three Lactobacillus strains (Limosilactobacillus reuteri KGC1901, Lacticaseibacillus casei KGC1201, Limosilactobacillus fermentum KGC1601) isolated from Panax ginseng, using HT-29 cells, mucin-coated plates, and human pluripotent stem cell-derived intestinal epithelial cells as in vitro models of human gut physiology. We observed that the multilayer-coated strains displayed improved survival rates after passage through gastric juice, as well as high adhesion and proliferation capacities within the various gut epithelial systems tested, compared to their uncoated counterparts. Our findings demonstrated that the multilayer coat effectively protected commensal microbiota and led to improved adhesion and colonization of intestinal epithelial cells, and consequently to higher probiotic efficacy.
참고문헌 (36)
- Kwon ML, "Exopolysaccharide isolated from Lactobacillus plantarum L-14 has antiinflammatory effects via the toll-like receptor 4 pathway in LPS-induced RAW 264.7 cells", Int. J. Mol. Sci, 21, pp.9283~ (2020)
- Jeon HJY, "Multilayer coatings containing red ginseng dietary fibre improve the survivability and stability of probiotic bacteria", Institute of Food Technologists, ~ (2023)
- Aadil MMANRBSMBPŁKQSMUMFMSMZSYRM, "Nutritional and Health Potential of Probiotics: A Review", 11, pp.11204~ (2013)
- Dronkers TMG, "Global analysis of clinical trials with probiotics", Heliyon, 6, pp.e04467~ (2020)
- Kobayashi SGYSMN, "Therapeutics and immunoprophylaxis against noroviruses and rotaviruses: The past, present, and future", Curr. Drug Metab, 19, pp.170~191 (2018)
- Salman Shirvani-Rad, "Probiotics as a complementary therapy for management of obesity: A systematic review", Evid. Based Complement. Alternat. Med, 2021, pp.6688450~ (2021)
- Meng XC, "Anhydrobiotics: The challenges of drying probiotic cultures", Food Chem, 106, pp.1406~1416 (2007)
- Schutyser JPLBMBFJHKMKRMBMAI, "Dehydration and thermal inactivation of Lactobacillus plantarum WCFS1:Comparing single droplet drying to spray and freeze drying", Food Res. Int, 54, pp.1351~1359 (2013)
- Kim KM, "Probiotic properties and immune-stimulating effect of the Jeju lava seawater mineral-coated probiotics", LWT, 126, pp.109299~ (2020)
- Mendonca AA, "Journey of the probiotic bacteria:Survival of the fittest", Microorganisms, 11, pp.95~ (2022)
- Qing Liu, "Surface components and metabolites of probiotics for regulation of intestinal epithelial barrier", Microb. Cell Fact, 19, pp.23~ (2020)
- Elloise du Toit, "Assessment of the effect of stress-tolerance acquisition on some basic characteristics of specific probiotics", Int. J. Food Microbiol, 165, pp.51~56 (2013)
- Tetsuji Hori, "Probiotics: A dietary factor to modulate the gut microbiome, host immune system, and gut-brain interaction", Microorganisms, 8, pp.1401~ (2020)
- Kwon O, "The development of a functional human small intestinal epithelium model for drug absorption", Sci. Adv, 7, pp.eabh1586~ (2021)
- Yu HY, "Characterization of Limosilactobacillus reuteri KGC1901 newly isolated from Panax ginseng root as a probiotic and its safety assessment", Fermentation, 9, pp.228~ (2023)
- Lee Yun-Seok, "Probiotic Characteristics and Safety Assessment of Lacticaseibacillus casei KGC1201 Isolated from Panax ginseng", Journal of Microbiology and Biotechnology, 33(4), pp.519~526 (2023), 한국미생물·생명공학회[DOI]
- Kim H, "Anti-inflammatory effects of Limosilactobacillus fermentum KGC1601isolated from panax ginseng and its probiotic characteristics", Foods, 11, pp.1707~ (2022)
- Zuberer DA, "Recovery and enumeration of viable bacteria", Microbiol. Biochem Properties, 5, pp.119~144 (1994)
- Jung KB, "Interleukin-2 induces the in vitro maturation of human pluripotent stem cell-derived intestinal organoids", Nat. Cmmun, 9, pp.3039~ (2018)
- Serigado JM, "Ulcerative colitis: Novel epithelial insights provided by single cell RNA sequencing", Front. Med (Lausanne), 9, pp.868508~ (2022)
- Ghosh JLHDCSD, "Physicochemical properties of mucus and their impact on transmucosal drug delivery", Int. J. Pharm, 532, pp.555~572 (2017)
- 권기철, "Effect of Silk Fibroin Biomaterial Coating on Cell Viability and Intestinal Adhesion of Probiotic Bacteria", Journal of Microbiology and Biotechnology, 31(4), pp.592~600 (2021), 한국미생물·생명공학회[DOI]
- Zheng-Fei Yan, "Enhancement of rice protein hydrolysate quality using a novel dual enzyme system", LWT, 158, pp.113110~ (2022)
- Arellano Karina A., "Improving the Viability of Freeze-dried Probiotics Using a Lysine-based Rehydration Mixture", 한국미생물·생명공학회지, 49(2), pp.157~166 (2021), 한국미생물·생명공학회[DOI]
- Huang S, "Influence of arginine on the growth, arginine metabolism and amino acid consumption profiles of Streptococcus thermophilus T1C2 in controlled pH batch fermentations", J. Appl. Microbiol, 123, pp.746~756 (2016)
- Laetitia Rodes, "Transit time affects the community stability of Lactobacillus and Bifidobacterium species in an in vitro model of human colonic microbiotia", Artif. Cells Blood Substit. Biotechnol, 39, pp.351~356 (2011)
- Rine Christopher Reuben, "Isolation, characterization, and assessment of lactic acid bacteria toward their selection as poultry probiotics", BMC Microbiol, 19, pp.253~ (2019)
- Warnakulasuriya MADB Fernando, "The influence of environmental factors on the adhesion of combinations of probiotics to rice fibre fractions", World J. Microbiol. Biotechnol, 28, pp.2293~2302 (2012)
- Hye-Young Yu, "Growth promotion effect of red ginseng dietary fi ber to probiotics and transcriptome analysis of Lactiplantibacillus plantarum", Journal of Ginseng Research, 47(1), pp.159~165 (2023), 고려인삼학회
- Chongde Wu, "Mechanisms and improvement of acid resistance in lactic acid bacteria", Arch. Microbiol, 200, pp.195~201 (2018)
- Chao Wang, "A combined physiological and proteomic approach to reveal lactic-acid-induced alterations in Lactobacillus casei Zhang and its mutant with enhanced lactic acid tolerance", Appl. Microbiol. Biotechnol, 93, pp.707~722 (2012)
- Chatterjee M, "Understanding the adhesion mechanism of a mucin binding domain from Lactobacillus fermentum and its role in enteropathogen exclusion", Int. J. Biol. Macromol, 110, pp.598~607 (2018)
- Dudík B, "Mucin pre-cultivated Lactobacillus reuteri E shows enhanced adhesion and increases mucin expression in HT-29 cells", Antonie Van Leeuwenhoek, 113, pp.1191~1200 (2020)
- Araújo F, "Towards the characterization of an in vitro triple co-culture intestine cell model for permeability studies", Int. J. Pharm, 458, pp.128~134 (2013)
- Gagnon M, "Comparison of the Caco-2, HT-29 and the mucus-secreting HT29-MTX intestinal cell models to investigate Salmonella adhesion and invasion", J. Microbiol. Methods, 94, pp.274~279 (2013)
- Srinivasan B, "TEER measurement techniques for in vitro barrier model systems", J. Lab. Autom, 20, pp.107~126 (2015)