Effect of Silk Fibroin Biomaterial Coating on Cell Viability and Intestinal Adhesion of Probiotic Bacteria
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Probiotics can be processed into a powder, tablet, or capsule form for easy intake. They are exposed to frequent stresses not only during complex processing steps, but also in the human body after intake. For this reason, various coating agents that promote probiotic bacterial stability in the intestinal environment have been developed. Silk fibroin (SF) is a material used in a variety of fields from drug delivery systems to enzyme immobilization and has potential as a coating agent for probiotics. In this study, we investigated this potential by coating probiotic strains with 0.1% or 1% water-soluble calcium (WSC), 1% SF, and 10% trehalose. Under simulated gastrointestinal conditions, cell viability, cell surface hydrophobicity, and cell adhesion to intestinal epithelial cells were then measured. The survival ratio after freeze-drying was highest upon addition of 0.1% WSC. The probiotic bacteria coated with SF showed improved survival by more than 10.0% under simulated gastric conditions and 4.8% under simulated intestinal conditions. Moreover, the cell adhesion to intestinal epithelial cells was elevated by 1.0-36.0%. Our results indicate that SF has positive effects on enhancing the survival and adhesion capacity of bacterial strains under environmental stresses, thus demonstrating its potential as a suitable coating agent to stabilize probiotics throughout processing, packaging, storage and consumption.
Abstract
Probiotics can be processed into a powder, tablet, or capsule form for easy intake. They are exposed to frequent stresses not only during complex processing steps, but also in the human body after intake. For this reason, various coating agents that promote probiotic bacterial stability in the intestinal environment have been developed. Silk fibroin (SF) is a material used in a variety of fields from drug delivery systems to enzyme immobilization and has potential as a coating agent for probiotics. In this study, we investigated this potential by coating probiotic strains with 0.1% or 1% water-soluble calcium (WSC), 1% SF, and 10% trehalose. Under simulated gastrointestinal conditions, cell viability, cell surface hydrophobicity, and cell adhesion to intestinal epithelial cells were then measured. The survival ratio after freeze-drying was highest upon addition of 0.1% WSC. The probiotic bacteria coated with SF showed improved survival by more than 10.0% under simulated gastric conditions and 4.8% under simulated intestinal conditions. Moreover, the cell adhesion to intestinal epithelial cells was elevated by 1.0-36.0%. Our results indicate that SF has positive effects on enhancing the survival and adhesion capacity of bacterial strains under environmental stresses, thus demonstrating its potential as a suitable coating agent to stabilize probiotics throughout processing, packaging, storage and consumption.
참고문헌 (48)
- Morelli L, "FAO", WHO Guidelines on probiotics, 2012, pp.46, S1~
- Williams NT, "Probiotics", Am. J. Health-Syst. Pharm, 67, pp.449~458 (2010)
- Szajewska H, "Probiotics in the treatment and prevention of acute infectious diarrhea in infants and children: a systematic review of published randomized, double-blind, placebo-controlled trials", J. Pediatr. Gastroenterol. Nutr, 33, pp.S17~S25 (2001)
- Sartor RB, "Therapeutic manipulation of the enteric microflora in inflammatory bowel diseases : antibiotics, probiotics, and prebiotics", Gastroenterol, 126, pp.1620~1633 (2004)
- George KR, "Benefaction of probiotics for human health : a review", J. Food Drug Anal, 26, pp.927~939 (2018)
- Sanders ME, "Probiotics and prebiotics in intestinal health and disease : from biology to the clinic", Nat. Rev. Gastroenterol. Hepatol, 16, pp.605~616 (2019)
- Sarao LK, "Probiotics, prebiotics, and microencapsulation : a review", Crit. Rev. Food Sci. Nutr, 57, pp.344~371 (2017)
- de Vrese M, "Probiotics, prebiotics and synbiotics", Adv. Biochem. Eng. Biotechnol, 111, pp.1~66 (2008)
- Tripathi MK, "Probiotic functional foods : survival of probiotics during processing and storage", J. Funct. Foods, 9, pp.225~241 (2014)
- Fiocco D, "How probiotics face food stress : they get by with a little help", Crit. Rev. Food Sci. Nutr, 60, pp.1552~1580 (2020)
- Anselmo AC, "Layer-by-layer encapsulation of probiotics for delivery to the microbiome", Adv. Mater, 28, pp.9486~9490 (2016)
- Burgain J, "Encapsulation of probiotic living cells : from laboratory scale to industrial applications", J. Food Eng, 104, pp.467~483 (2011)
- Yucel FC, "Hybrid coating of alginate microbeads based on proteinbiopolymer multilayers for encapsulation of probiotics", Biotechnol. Prog, 35, pp.1~12 (2019)
- Kim KM, "Probiotic properties and immune-stimulating effect of the Jeju lava seawater mineral-coated probiotics", LWT-Food Sci. Technol, 126, pp.1~6 (2020)
- Xiao Y, "Encapsulation of Lactobacillus rhamnosus in hyaluronic acid-based hydrogel for pathogen-targeted delivery to ameliorate enteritis", ACS Appl. Mater. Interfaces, 12, pp.36967~36977 (2020)
- Gerez CL, "Whey protein coating bead improves the survival of the probiotic Lactobacillus rhamnosus CRL 1505 to low pH", Lett. Appl. Microbiol, 54, pp.552~556 (2012)
- Ding WK, "Effect of various encapsulating materials on the stability of probiotic bacteria", J. Food Sci, 74, pp.100~107 (2009)
- Pliszczak D, "Improvement of an encapsulation process for the preparation of pro-and prebiotics-loaded bioadhesive microparticles by using experimental design", Eur. J. Pharm. Sci, 44, pp.83~92 (2011)
- Apostolou E, "Good adhesion properties of probiotics: a potential risk for bacteremia?", FEMS Immunol. Med. Microbiol, 31, pp.35~39 (2001)
- Monteagudo-Mera A, "Adhesion mechanisms mediated by probiotics and prebiotics and their potential impact on human health", Appl. Microbiol. Biotechnol, 103, pp.6463~6472 (2019)
- Falah F, "Evaluation of adherence and anti-infective properties of probiotic Lactobacillus fermentum strain 4-17 against Escherichia coli causing urinary tract infection in humans", Microb. Pathog, 131, pp.246~253 (2019)
- Mondal M, "The silk proteins, sericin and fibroin in silkworm, Bombyx mori Linn, -a review", Casp. J. Environ. Sci, 5, pp.63~76 (2007)
- Qi Y, "A review of structure construction of silk fibroin biomaterials from single structures to multi-level structures", Int. J. Mol. Sci, 18, pp.237~ (2017)
- Nguyen TP, "Silk fibroin-based biomaterials for biomedical", Polymers, 11, pp.1~25 (2019)
- Chouhan D, "Silk biomaterials in wound healing and skin regeneration therapeutics : from bench to bedside", Acta Biomater, 103, pp.24~51 (2020)
- Byun EB, "Enhancement of anti-tumor activity of gamma-irradiated silk fibroin via immunomodulatory effects", Chem. -Biol. Interact, 186, pp.90~95 (2010)
- Coeuret V, "Isolation, characterisation and identification of lactobacilli focusing mainly on cheeses and other dairy products", EDP Sciences, 83, pp.269~306 (2003)
- Devereux R, "Mol. Microb. Ecol. Manual" (2004), Springer
- Nogueira GM, "Preparation and characterization of ethanoltreated silk fibroin dense membranes for biomaterials application using waste silk fibers as raw material", Bioresour. Technol, 101, pp.8446~8451 (2010)
- Celik OF, "Factors influencing the stability of freeze-dried stress-resilient and stress-sensitive strains of bifidobacteria", Int. J. Dairy Sci, 96, pp.3506~3516 (2013)
- Hansen LT, "Survival of Ca-alginate microencapsulated Bifidobacterium spp. in milk and simulated gastrointestinal conditions", Food Microbiol, 19, pp.35~45 (2002)
- Zuberer DA, "Methods of Soil Analysis: Part 2 Microbiological and Biochemical Properties", pp.119~144 (1994), Wiley
- Krausova G, "In vitro evaluation of adhesion capacity, hydrophobicity, and auto-aggregation of newly isolated potential probiotic strains", Fermentation, 5, pp.100~ (2019)
- Bustos I, "Effect of flavan-3-ols on the adhesion of potential probiotic lactobacilli to intestinal cells", J. Agric. Food Chem, 60, pp.9082~9088 (2012)
- Hirano J, "The effect of Lactobacillus rhamnosus on enterohemorrhagic Escherichia coli infection of human intestinal cells in vitro", Microbiol. Immunol, 47, pp.405~409 (2003)
- Alemka A, "Probiotic colonization of the adherent mucus layer of HT29MTXE12 cells attenuates Campylobacter jejuni virulence properties", Infect. Immun, 78, pp.2812~2822 (2010)
- Zacarías MF, "Influence of technological treatments on the functionality of Bifidobacterium lactis INL1, a breast milk-derived probiotic", J. Food Sci, 82, pp.2462~2470 (2017)
- Ainsley Reid A, "Microentrapment of probiotic bacteria in a Ca2+-induced whey protein gel and effects on their viability in a dynamic gastro-intestinal model", J. Microencapsul, 22, pp.603~619 (2005)
- Zheng X, "Exploring the protective effects of calcium-containing carrier against dryinginduced cellular injuries of probiotics using single droplet drying technique", Food Res. Int, 90, pp.226~234 (2016)
- Nogueira GM, "Hydrogels from silk fibroin metastable solution : formation and characterization from a biomaterial perspective", Mater. Sci. Eng. C, 31, pp.997~1001 (2011)
- de la Cruz Pech-Canul A, "A brief review of edible coating materials for the microencapsulation of probiotics", Coatings, 10, pp.1~34 (2020)
- Zhang DD, "Preparation and characterization of electrospun poly(vinyl alcohol)", silk fibroin nanofibers as a potential drug delivery system, 2013, pp.709, 215~220
- Bernet MF, "Adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells and inhibition of enteropathogen-cell interactions", Appl. Environ. Microbiol, 59, pp.4121~4128 (1993)
- Andrews GP, "Mucoadhesive polymeric platforms for controlled drug delivery", Eur. J. Pharm. Biopharm, 71, pp.505~518 (2009)
- de Wouters T, "Adhesion potential of intestinal microbes predicted by physicochemical characterization methods", PLoS One, 10, pp.e0136437~ (2015)
- Duary RK, "Assessing the adhesion of putative indigenous probiotic lactobacilli to human colonic epithelial cells", Indian J. Med. Res, 134, pp.664~671 (2011)
- Hojjati M, "Aggregation, adherence, anti-adhesion and antagonistic activity properties relating to surface charge of probiotic Lactobacillus brevis gp104 against Staphylococcus aureus", Microb. Pathog, 147, pp.104420~ (2020)
- Grigoryan S, "Aggregation and adhesion activity of lactobacilli isolated from fermented products in vitro and in vivo : a potential probiotic strain", Probiotics Antimicrob. Proteins, 10, pp.269~276 (2018)