Multifunctional Probiotic and Functional Properties of Lactiplantibacillus plantarum LRCC5314, Isolated from Kimchi
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In this study, the survival capacity (acid and bile salt tolerance, and adhesion to gut epithelial cells) and probiotic properties (enzyme activity-inhibition and anti-inflammatory activities, inhibition of adipogenesis, and stress hormone level reduction) of Lactiplantibacillus plantarum LRCC5314, isolated from kimchi (Korean traditional fermented cabbage), were investigated. LRCC5314 exhibited very stable survival at ph 2.0 and in 0.2% bile acid with 89.9% adhesion to Caco-2 intestinal epithelial cells after treatment for 2 h. LRCC5314 also inhibited the activities of α-amylase and α-glucosidase, which are involved in elevating postprandial blood glucose levels, by approximately 72.9% and 51.2%, respectively. Treatment of lipopolysaccharide (LPS)-stimulated RAW 264.7 cells with the LRCC5314 lysate decreased the levels of the inflammatory factors nitric oxide, tumor necrosis factor (TNF-α), interleukin (IL)-1β, and interferon-γ by 88.5%, 49.3%, 97.2%, and 99.8%, respectively, relative to those of the cells treated with LPS alone. LRCC5314 also inhibited adipogenesis in differentiating preadipocytes (3T3-L1 cells), showing a 14.7% decrease in lipid droplet levels and a 74.0% decrease in triglyceride levels, as well as distinct reductions in the mRNA expression levels of adiponectin, FAS, PPAR/γ, C/EBPα, TNF-α, and IL-6. Moreover, LRCC5314 reduced the level of cortisol, a hormone with important effect on stress, by approximately 35.6% in H295R cells. L. plantarum LRCC5314 is identified as a new probiotic with excellent in vitro multifunctional properties. Subsequent in vivo studies may further demonstrate its potential as a functional food or pharmabiotic.
Abstract
In this study, the survival capacity (acid and bile salt tolerance, and adhesion to gut epithelial cells) and probiotic properties (enzyme activity-inhibition and anti-inflammatory activities, inhibition of adipogenesis, and stress hormone level reduction) of Lactiplantibacillus plantarum LRCC5314, isolated from kimchi (Korean traditional fermented cabbage), were investigated. LRCC5314 exhibited very stable survival at ph 2.0 and in 0.2% bile acid with 89.9% adhesion to Caco-2 intestinal epithelial cells after treatment for 2 h. LRCC5314 also inhibited the activities of α-amylase and α-glucosidase, which are involved in elevating postprandial blood glucose levels, by approximately 72.9% and 51.2%, respectively. Treatment of lipopolysaccharide (LPS)-stimulated RAW 264.7 cells with the LRCC5314 lysate decreased the levels of the inflammatory factors nitric oxide, tumor necrosis factor (TNF-α), interleukin (IL)-1β, and interferon-γ by 88.5%, 49.3%, 97.2%, and 99.8%, respectively, relative to those of the cells treated with LPS alone. LRCC5314 also inhibited adipogenesis in differentiating preadipocytes (3T3-L1 cells), showing a 14.7% decrease in lipid droplet levels and a 74.0% decrease in triglyceride levels, as well as distinct reductions in the mRNA expression levels of adiponectin, FAS, PPAR/γ, C/EBPα, TNF-α, and IL-6. Moreover, LRCC5314 reduced the level of cortisol, a hormone with important effect on stress, by approximately 35.6% in H295R cells. L. plantarum LRCC5314 is identified as a new probiotic with excellent in vitro multifunctional properties. Subsequent in vivo studies may further demonstrate its potential as a functional food or pharmabiotic.
참고문헌 (42)
- Kamlesh Singh, "Probiotics: A review", Asian Pacific Journal of Tropical Biomedicine, 1(2), pp.S287~S290 (2011), Medknow[DOI]
- Erika Isolauri, "Probiotics: effects on immunity", The American Journal of Clinical Nutrition, 73(2), pp.444s~450s (2001), Elsevier BV[DOI]
- Leininger DJ, "Use of eosin methylene blue agar to differentiate Escherichia coli from other gramnegative mastitis pathogens", J. Vet. Diagn. Invest, 13, pp.273~275 (2001)
- Silva M, "Antimicrobial substance from a human Lactobacillus strain", Antimicrob. Agents Chemother, 31, pp.1231~1233 (1987)
- Antoine Aoun, "The Influence of the Gut Microbiome on Obesity in Adults and the Role of Probiotics, Prebiotics, and Synbiotics for Weight Loss", Preventive Nutrition and Food Science, 25(2), pp.113~123 (2020), 한국식품영양과학회[DOI]
- Mohajeri MH, "Relationship between the gut microbiome and brain function", Nutr. Rev, 76, pp.481~496 (2018)
- Valdes AM, "Role of the gut microbiota in nutrition and health", BMJ, 361, pp.2179~ (2018)
- Casey DE, "Antipsychotic-induced weight gain and metabolic abnormalities : implications for increased mortality in patients with schizophrenia", J. Clin. Psychiatry 65 Suppl, 7, pp.4~18 (2004)
- Yadav R, "An overview of advanced technologies for selection of probiotics and their expediency : a review", Crit. Rev. Food Sci. Nutr, 57, pp.3233~3242 (2017)
- Cammarota M, "In vitro evaluation of Lactobacillus plantarum DSMZ 12028 as a probiotic : emphasis on innate immunity", Int. J. Food Microbiol, 135, pp.90~98 (2009)
- DeVries MC, "Lactobacillus plantarum survival, functional and potential probiotic properties in the human intestinal tract", Int. Dairy J, 16, pp.1018~1028 (2006)
- Scholz E, "Karl Fischer. In Karl Fischer Titration", pp.1~2 (1984), Springer
- Lim JH, "Probiotic properties of Lactobacillus plantarum LRCC5193, a plant-origin lactic acid bacterium isolated from Kimchi and its use in chocolates", J. Food Sci, 83, pp.2802~2811 (2018)
- Sookkhee SM, "Lactic acid bacteria from healthy oral cavity of Thai volunteers : inhibition of oral pathogens", J. Appl. Microbiol, 90, pp.172~179 (2001)
- Strahinic I, "Molecular and biochemical characterizations of human oral lactobacilli as putative probiotic candidates", Oral Microbiol. Immunol, 22, pp.111~117 (2007)
- M. Bosch, "Probiotic properties of Lactobacillus plantarum CECT 7315 and CECT 7316 isolated from faeces of healthy children", Letters in Applied Microbiology, 54(3), pp.240~246 (2012), Oxford University Press (OUP)[DOI]
- 23S rRNA sequencing, "16S", pp.1994, Lane D
- Xiao Z, "A quantitative starch-iodine method for measuring alpha-amylase and glucoamylase activities", Anal. Biochem, 351, pp.146~148 (2006)
- Shai LJ, "Inhibitory effects of five medicinal plants on rat alpha-glucosidase : comparison with their effects on yeast alpha-glucosidase", J. Med. Plants Res, 5, pp.2863~2867 (2011)
- Mosmann T, "Rapid colorimetric assay for cellular growth and survival : application to proliferation and cytotoxicity assays", J. Immunol. Methods, 65, pp.55~63 (1983)
- Katherine S. Robbins, "Effect of pecan phenolics on the release of nitric oxide from murine RAW 264.7 macrophage cells", Food Chemistry, 212, pp.681~687 (2016), Elsevier BV[DOI]
- Satoru Sakuma, "Curcumin inhibits adipogenesis induced by benzyl butyl phthalate in 3T3-L1 cells", Toxicology and Applied Pharmacology, 329, pp.158~164 (2017), Elsevier BV[DOI]
- Dusch H, "Evaluation of five new plating media for isolation of Salmonella species", J. Clin. Microb, 33, pp.802~804 (1995)
- Saarela M, "Probiotic bacteria : safety, functional and technological properties", J. Biotechnol, 84, pp.197~215 (2000)
- Mcdonald LC, "Acid tolerance of Leuconostoc mesenteroides and Lactobacillus casei", Appl. Environ. Microb, 53, pp.2124~2128 (1990)
- Gilliland SE, "Importance of bile tolerance of Lactobacillus acidophilus used as a dietary adjunct", J. Dairy Sci, 67, pp.3045~3051 (1984)
- Salminen SE, "Clinical uses of probiotics for stabilizing the gut mucosal barrier : successful strains and future challenges", Antonie Van Leeuwenhoek, 70, pp.347~358 (1996)
- Bernet MF, "Lactobacillus acidophilus LA 1 binds to cultured human intestinal cell lines and inhibits cell attachment and cell invasion by enterovirulent bacteria", Gut, 35, pp.483~489 (1994)
- Ali H, "Alpha-amylase inhibitory activity of some Malaysian plants used to treat diabetes;with particular reference to Phyllanthus amarus", J. Ethnopharmacol, 107, pp.449~455 (2006)
- Kinariwala D, "Exploring the potentiality of Lactobacillus cultures on the production of milkderived bioactive peptides with antidiabetic activity", Int. J. Pept. Res. Ther, 26, pp.1613~1627 (2020)
- Kimoto H, "New Lactococcus strain with immunomodulatory activity : enhancement of Th1-type immune response", Microbiol. Immunol, 48, pp.75~82 (2004)
- Laskin DL, "Macrophages and inflammatory mediators in chemical toxicity : a battle of forces", Chem. Res. Toxicol, 22, pp.1376~1385 (2009)
- Oh NS, "Probiotic and anti-inflammatory potential of Lactobacillus rhamnosus 4B15 and Lactobacillus gasseri 4M13 isolated from infant feces", PLoS One, 13, pp.e0192021~ (2018)
- Weninger W, "Chemokine regulation of naïve T cell traffic in health and disease", Semin. Immunol, 15, pp.257~270 (2003)
- Frayn KN, "Integrative physiology of human adipose tissue", Int. J. Obes. Relat. Metab. Disord, 27, pp.875~888 (2003)
- Mishra AP, "Bioactive compounds and health benefits of edible Rumex species-a review", Cell. Mol. Biol, 64, pp.27~34 (2018)
- Cowherd RM, "Molecular regulation of adipocyte differentiation", Semin. Cell. Dev. Biol, 10, pp.3~10 (1999)
- Rosen ED, "Transcriptional regulation of adipogenesis", Genes Dev, 14, pp.1293~1307 (2000)
- Cornelius POA, "Regulation of adipocyte development", Annu. Rev. Nutr, 14, pp.99~129 (1994)
- 강창호, "한국인 여성 및 식품 유래 유산균의 생리적 특성 및 지방분화 억제효과", KSBB Journal, 33(3), pp.161~167 (2018), 한국생물공학회[DOI]
- Sorrenti V, "Beneficial effects of pomegranate peel extract and probiotics on pre-adipocyte differentiation", Front. Microbiol, 10, pp.660~ (2019)
- Hoffman JR, "The effect of environmental temperature on testosterone and cortisol responses to high intensity, intermittent exercise in humans", Eur. J. Appl. Physiol. Occup. Physiol, 75, pp.83~87 (1997)