생쥐 경부림프절에서 면역 B 림프구의 특성
Characteristic Features of Immune B Cells in Murine Cervical Lymph Node
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Abstract
Background and Objectives：To investigate the characteristic features of B cels in murine cervical lymph node. Animal ex-periment. Subjects and Method：We obtained cervical lymph node of mice. Isolated B cels were cultured in media after the cytometry. Imunoglobulin secreted into the culture supernatants was evaluated by enzyme-linked immunosorbent asay. B20+ cells cultured for 5 days with media alone, or LPS. Entering into S phase in response to stimuli was measured by proliferative assay. Results：Identifying phenotypic characteristics for B cels of lymph node include CD5low, CD23high, CD43low, B20high, Ig (sIg)Mlow, sIgDhigh, CD80 ( )low, CD86 (B7.2)low, and Syndecam-1low. Spontaneous Imunoglobulin production did not occur in lymphoid B cels. When stimulation, IgM secretion was increased more than that of IgA and IgG. Lipopolysaccharide is a most powerful stimulus which secreted immunoglobulin. In thymidine incorporation, B cels were entered actively S phase after 48hours stimulation. CD40 plus anti-CD8 stimulated B cels to produce substantial thymidine incorporation that was much Conclusion：The B cels in cervical lymph node are conventional B cels (B2). Lymph node B cells secreted mainly IgM at stimuli and cell proliferation was activated at 48 hours of stimulation.
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