덱사메타손이 생쥐 B세포의 세포 표면 인식자와 분화에 미치는 영향
Effect of Dexamethasone on the Surface Expression of Marker Molecules and Differentiation of Murine B Cells
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Abstract
Background: There are at least two different subsets of B cells, B-1 and B-2. The characteristic features and function of B-2 cells in addition to the effect of steroids on B-2 cells are well-known. Although B-1 cells have different features and functions from B-2 cells, the effect of steroids on B-1 cells is not completely understood. Therefore, this study examined the effects of dexamethasone on peritoneal (or B-1 cells) and splenic B cells (or B-2 cells). Methods: Purified B cells were obtained from the peritoneal fluid and the spleens of mice. The isolated B cells were cultured in a medium and after adding different concentrations of dexamaethasone. The cell survival rate was measured by flow cytometry using propidium iodide. The expression level of the B cell surface marker was analyzed by flow cytometry. During the culture of these cells, immunoglobulin secreted into the culture supernatants was evaluated by an enzyme-linked immunosorbent assay. Results: The survival rate of peritoneal and splenic B cells decreased with increasing dexamethasone concentration. However, the rate of peritoneal B cell apoptosis was lower than that of splenic B cells. CD5 and B7.1 expression in peritoneal B cells and CD23 and sIgM expression in splenic B cells after the dexamethasone treatment were reduced. When B cells were treated with dexamethasone, the spontaneous IgM secretion decreased with increasing dexamethasone concentration. Conclusion: Dexamethasone induces apoptosis in peritoneal and splenic B cells. However, peritoneal B cells are less sensitive to dexamethasone. The dexamethasone suppressed expression of the surface markers in peritoneal B cells is different from those in splenic B cells. (Immune Network 2006;6(3):138-144)
참고문헌 (20)
- "Anti-inflammatory actions of glucocorticoids", 94, pp.557~572 (1998)
- "The mode of action of immunosuppressive agents research monographs in immunology", pp.~104 (1985)
- "Corticosteroids and lymphoid cells", 287, pp.388~397 (1972)
- "New insights into the molecular mechanisms of corticosteroids actions", 7, pp.649~660 (2006)
- "Cutting edge commentary", 168, pp.4257~4261 (2002)
- "Induction of apoptosis by dexamethasone in the B cell lineage", 40, pp.67~76 (1998)
- "The effect of corticosteroids upon murine B cells in vivo and in vitro as determined in the LPS-culture system", 62, pp.285~290 (1987)
- "Anti-inflammatory actions of glucocorticoids", 94, pp.557~572 (1998)
- "B-1 cells differ from conventional B (B-2) cells", 4, pp.155~160 (2004)
- "Characteristic features of immune B cells in murine cervical lymph node", 48, pp.241~246 (2005)
- "The modulation of B7.2 and B7.1 on B cells by immunosuppressive agents", 118, pp.1~8 (1999)
- "Effects of alpha-interferon and steroids on CD23 expression and release in B-cell chronic lymphocytic leukemia", 79, pp.205~212 (1994)
- "Corticosteroids, IgE, and atopy", 107, pp.265~266 (2001)
- "Regulation of CD23 expression soluble CD23 release and immunoglobulin synthesis of peripheral blood lymphocytes by glucocorticoids", immu(l 71), pp.473~479 (1990)
- "Interleukin 5 enhances interleukin 4-induced IgE production by normal human B cells", 18, pp.929~935 (1998)
- "The effect of glucocorticoids on mitogen-stimulated B lymphocytes", pp.169~175 (1982), thymidine incorporation and antibody secretion Endocrinology 110
- "Cortisone action on serum colony-stimulating factor and bone marrow in vitro colony-forming cells", 132, pp.391~394 (1969)
- "Marrow lymphocyte production during chronic hydrocortisone administration", 23, pp.111~118 (1978)
- "The effect of glucocorticosteroids on the proliferation and cinetics of promonocytes and monocytes of the bone marrow", 137, pp.10~21 (1973)
- "Induction of immunoglobulin and antibody synthesis in vitro by lipopolysaccharide", 2, pp.349~353 (1972)