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아토피성 피부질환 동물 모델 NC/Nga 생쥐에서 내재면역 T와 B 세포의 변형

Alteration of Innate Immune T and B Cells in the NC/Nga Mouse

김효정|김태윤|박세호|홍석만|김정은
대한면역학회 (The Korean Association of Immunologists)|2005.09|Immune Network|제5권 제3호|pp. 137-143

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Abstract

Background: Millions of people in the world are suffering from atopic dermatitis (AD), which is a chronic inflammatory skin disease triggered by Th2 immune responses. The NC/Nga mouse is the most extensively studied animal model of AD. Like human AD, NC/Nga mice demonstrate increased levels of IgE, a hallmark of Th2 immune responses. Adaptive immunity cannot be generated without help of innate immunity. Especially natural killer T (NKT) cells and marginal zone B (MZB) cells have been known to play important roles in linking innate immunity to adaptive immunity. Methods: Through flow cytometric analysis and ELISA assay, we investigated whether these lymphocytes might be altered in number in NC/Nga mice. Results: Our data demonstrated that the number of NKT cells was reduced in NC/Nga mice and IFNγ production by NKT cells upon α-GalCer stimulation decreased to the levels of CD1d KO mice lacking in NKT cells. However, reduction of NKT cells in NC/Nga mice was not due to CD1d expression, which was normal in the thymus. Interestingly, there was a significant increase of CD1dhighB220 cells in the spleen of NC/Nga mice. Further, we confirmed that CD1dhighB220 cells are B cells, not dendritic cells. These CD1dhighB220 B cells show IgMhighCD21highCD23low, a characteristic phenotype of MZB cells. Conclusion: We provide the evidence that there are decreased activities of NKT cells and increased number of MZB cells in the NC/Nga mice. Our findings may thus explain why NC/Nga mice are susceptible to AD.

참고문헌 (30)

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