2-Aminoethoxydiphenyl Borate(2-APB)를 이용한 IP3 수용체억제가 와우유모세포의 Gentamicin에 의한 이독성에 미치는 영향
The Effect of IP3 Receptor Inhibition Using 2-APB on GentamicinOtotoxicity in Cochlear Sensory Cell
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Abstract
Background and Objectives：Elevated intracellular calcium level is known to play important roles in the apoptotic pathway. IP3 receptor (ligand-gated channels that release Ca2+ from intracellular stores) is emerging as a key site for regulation of apo-ptosis. 2-Aminoethoxydiphenyl borate (2-APB)APB on gentamicin ototoxicity in vitro, using the HEI-OC1 cell line. Materials and Method：HEI-OC1 cells were treated with 100μM gentamicin. Using a CaspACE assay, we measured the caspases-3 activity in the gentamicin treated hair cells with and without 2-APB pre-incubation. We also observed intra-cellular calcium concentrations in HEI-OC1 cels using a confocal micro-scopy (calcium gren-1 stain). Live cel imaging was performed by using fluorescence video-time lapse system. Results：Cytosolic calcium elevation by gentamicin was remarkably inhibited by 2-APB. Caspases-3 activities of gentamicin treated cells APB, caspases-3 activities and cel death of gentamicin treated cels were shown to decrease. Conclusion：2-APB reduces Caspases-3 activity in the gentamicin treated HEI-OC1 cells by inhibi-tion of cytosolic calcium increase. (Korean J Otolaryngol 2007 ;50 :385-90)
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