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전정 유모세포에서 Minocycline 전 처리를 통한 Neomycin 이독성예방효과；Reactive Oxygen Species와 Caspase-3 활성억제

Minocycline Protects Vestibular Hair Cells from Neomycin-Induced Ototoxicity byInhibiting Reactive Oxygen Species Production and Caspase-3 Activity

정재윤|이정구|백인영|안진철|김영생
대한이비인후과학회 (Korean Society of Otorhinolaryngology-Head and Neck Surgery)|2006.12|대한이비인후과학회지 두경부외과학 (Korean Journal of Otorhinolaryngology-Head and Neck Surgery)|제49권 제12호|pp. 1159-1164

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참고문헌

키워드

미노사이클린·Caspase·활성산소·난형낭반Minocycline·Caspase-3·Reactive oxygen species·Utricle.
주제분류이비인후과학

초록

Abstract

Background and Objectives：Aminoglycosides, a commonly used antibiotic agent, destroys the sensory hair cells in the cochlearand vestibular system leading to irreversible hearing loss and balance problem. Minocycline, a second-generation tetracyclineantibiotic, has been known to possess anti-apoptotic properties in addition to its antimicrobial action. We hypothesized thatminocycline would attenuate aminoglycosides induced vestibulotoxicity in rat utricles. Subjects and Method：Utricular maculaeprepared from postnatal day 3-4 rats were treated with neomycin alone or in combination with minocycline. For hair cell count,utricles were stained with phalloidin- FITC. Reactive oxygen species (ROS) was assessed using the fluorescent probe, hydrofluorescentdiacetate acetyl ester (H2DCFDA). Caspase-3 activity was also examined using the fluorescent caspase-3 substrate.Results：Neomycin induced dose-dependent loss of utricular hair cells. Minocycline reduced ROS production and caspase-3activation in neomycin treated utricular hair cells. Conclusion：Minocycline has protective effect in neomycin induced ototoxicityin rat utricle by inhibiting ROS production and caspase-3 activity. (Korean J Otolaryngol 2006;49:1159-64)

참고문헌 (19)

  1. "Aminoglycoside antibiotics restore dystrophy function to skeletal musclesof mdx mice", 104(4), pp.375~381 (1999)
  2. "Aminoglycoside induced ototoxicity in patients with cystic fibrosis", pp.173~5
  3. "Minocycline and other tetracyclinederivatives: A neuprotective strategy in Parkinsons disease and Huntingtonsdisease", 26(1), pp.18~23 (2003)
  4. "Apoptotic death of hair cells in mammalian vestibularsensory epithelia", 139, pp.97~115 (2000)
  5. "Minocycline inhibits caspase activation and reactivation,increases the ratio of XIAP to smac", DIABLO, and reduces themitochondrial leakage of cytochrome c and smac, pp.2004, 43(5)[DOI]
  6. "Minocycline attenuategentamicin induced hair cell loss in neonatal cochlear cultures", 197, pp.11~18 (2004)
  7. "Dynamic studiesof ototoxicity in mature avian auditory epithelium", 884, pp.389~409 (1999)
  8. "Minocyclineprevents gentamicin-induced ototoxicity by inhibiting p38 MAP kinasephosphorylation and caspase-3 activation", 131(2), pp.513~521 (2005)
  9. "Aminoglycoside antibiotics", 5(1), pp.3~22 (2000)
  10. "Caspase activation in haircells of the mouse utricle exposed to neomycin", 22(19), pp.8532~8540 (2002)
  11. "A peptide inhibitor of c-jun N-terminal kinase protects against bothaminoglycoside and acoustic trauma-induced auditory hair cell deathand hearing loss", 113(24), pp.491~502 (2003)
  12. "Neuroprotectiveeffects of minocycline against in vitro and in vivo retinal ganglion celldamage", 1053, pp.185~194 (2005)
  13. "Minocyclineblocks 6-hydroxydopamine-induced neurotoxicity and free radical productionin rat cerebellar granule neurons", 72(14), pp.1635~1641 (2003)
  14. "Minocycline inhibits caspase-independent and dependent mitochondrialdell death pathways in models of Huntingtons disease", 100(18), pp.10483~10487 (2003)
  15. "Reactive oxygen species inchick hair cells after gentamicin exposure in vitro", 104, pp.1~14 (1997)
  16. "Protective effects of alpha-tocopherol against gentamicin-inducedOto-vestibulo toxicity: An experimental study", 123(2), pp.192~197 (2003)
  17. "Minocycline inhibits cytochrome c release and delays progression ofamytophic lateral sclerosis in mice", 417(6884), pp.74~78 (2002)
  18. "Antioxidants attenuate gentamicin-induced freeradical formation in vitro and ototoxicity in vivo: D-methionine is apotential protectant", 142, pp.34~40 (2000)
  19. "Caspase-3-dependent cleavage of Bcl-2 promotes releaseof cytochrome c", 274(30), pp.21155~21161 (1999)

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