흰쥐 유양동 폐쇄술에 사용한 물질의 효과 및 조직병리학적 비교 연구
Histopathologic Evaluation of Obliterating Materials in theTemporal Dorsal Bullae of Rat
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Abstract
Background and Objectives:Mastoid obliteration is the technique to reduce disadvantages of canal wall down procedure. Many materials for mastoid obliteration have ben devised. The aim of our study is to evaluate the histopathologic changes according to the diferent obliterating materials and removal of the mastoid mucosa in the temporal bulae of rat. Materials and Method:We divided rats into two groups. One group underwent removal of mastoid mucosa with 2% TCA (trichloroacetic acid), and the other was left untreated. Both groups had mastoid obliteration by implantation of bone wax, xenograft bone material (Lubboc), or ho-med to evaluate inflammation, foreign body response, new bone formation, implant material resorption, and mucocele formation within the bullae. Results:Histopatholgoic evaluation revealed many instances of inflamation and foreign body response in the groups using the bone wax. In rats implanted with xenograft bone material or homologous corticocancellous bone, by contrast, microscopic examination showed new woven bone ingrowth at the bone implant interface. Especialy, homologous corticocan-cellous bone showed good results. Rats not treated with 2% TCA showed the presence of mucocele. :We suggest that homologous corticocancellous bone is the better obliterating material than bone wax or xenograft bone material. And also, all mastoid lining mucosa should be removed prior to implantation in mastoid obliteration surgery. Further research will be neces-sary to determine the sequence of morphologic changes that occur in mastoid bullae mucosa. (Korean J Otolaryngol 2007 ;50 : 391-8)
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