뽕잎과 녹차혼합분말의 급여가 streptozotocin 유발 당뇨쥐의 항당뇨효과
Anti-diabetic effect of the mixture of mulberry leaf and green tea powder in rats with streptozotocin-induced diabetes
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뽕잎, 녹차 및 뽕잎과 녹차혼합물이 sterptozotocin 유발 당뇨 쥐의 혈당 조절효과에 대해 살펴보았다. 실험군은 정상군(N), 당뇨 대조군(STZ), 5% 뽕잎 분말 첨가 당뇨군(STZ-M), 5% 녹차 분말 첨가 당뇨군(STZ-G) 및 2.5% 뽕잎과 2.5% 녹차혼합분말 첨가 당뇨군(STZ-MG)으로 5군으로 나누어 4주간 실시하였다. 체중증가량은 뽕잎, 녹차 및 뽕잎과 녹차혼합물 첨가 당뇨군(STZ-M, STG-G, STZ-MG)들이 당뇨 대조군(STZ)에 비하여 유의적 차이는 없었으나 다소 증가하였다. 뇨 중 포도당 함량은 뽕잎, 녹차 및 뽕잎과 녹차혼합물 첨가 당뇨군(STG-G, STZ-MG)들이 당뇨 대조군(STZ)에 비하여 감소효과를 보였다. 4주간 혈당의 변화는 뽕잎, 녹차 및 뽕잎과 녹차혼합물(STZ-M, STG-G, STZ-MG)의 급여로 혈당 수준이 감소하는 경향이었고, 뽕잎과 녹차혼합물 첨가 당뇨군(STZ-MG)이 혈당 강하효과가 가장 높았다. 시험 종료 후 혈청 포도당 함량은 뽕잎, 녹차 및 뽕잎과 녹차혼합물 첨가(STZ-M, STG-G, STZ-MG)로 당뇨 대조군(STZ)에 비하여 모두 유의적으로 낮게 나타나 정상군(N)과 비슷한 경향을 보였다. 혈청 인슐린 함량은 녹차 및 뽕잎과 녹차혼합물 첨가(STG-G, STZ-MG)로 당뇨 대조군(STZ)에 비하여 유의적으로 증가하였고, 혈청 fructosamine 함량은 유의적으로 감소하였다. 이상의 결과 뽕잎, 녹차 및 뽕잎과 녹차혼합물의 급여는 STZ로 유발된 당뇨 쥐의 혈당 강하효과를 확인하여, 당뇨성 질환의 예방 및 개선에 효과가 있을 것으로 사료된다.
Abstract
This study investigated the hypoglycemic effects of mulberry leaf (M), green tea (G), and a mixture of mulberry leaf and green tea (MG) in rats with streptozotocin-induced diabetes. Male Sprague-Dawley rats were divided into the non-diabetic (N) and diabetic groups. The experimental animals were divided into four groups and fed the following for four weeks: a normal diet (N), the STZ+normal diet (STZ), the STZ+5% mulberry leaf (STZ-M), STZ+5% green tea (STZ-G), and the STZ+5% mixture of mulberry leaf and green tea (STZ-MG). The whole blood glucose level of the STZ-M, STZ-G and STZ-MG groups significantly decreased compared to the STZ group two weeks later. The serum glucose and fructosamine, and urine glucose levels in the STZ-M, STZ-G and STZ-MG groups were lower than in the STZ group. The levels of serum insulin were higher in the STZ-M, STZ-G and STZ-MG groups than in the STZ group. However, serum glucose, fructosamine and insulin levels, and urine glucose levels were not significantly different among the STZ-M, STZ-G and STZ-MG groups. These results indicate that mulberry leaf, green tea, and their mixture help prevent or attenuate the progression of diabetes in rats with STZ-induced diabetes.
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