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과산화수소수로 유도된 배양신경세포손상에 대한 참죽나무잎 추출물의 보호효과

Extract of Cedrela sinensis Leaves Protects Neuronal Cell Damage Induced by Hydrogen Peroxide in Cultured Rat Neurons

김주연|주현수|조순옥|반주연|성연희|배기환|이순복
한국약용작물학회|2007.12|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제15권 제6호|pp. 444-450

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참고문헌

키워드

hydrogen peroxideneuroprotectionCedrela sinensisneurotoxicitycortical neurons

초록

ABSTRACT : Dried leaves from Cedrela sinensis A. Juss. (CS), have been observed to possess various pharmacological activity and contain various antioxidant constituents. The protective effect of ethanol extract of CS on hydrogen peroxide (H 2 O 2 )-induced neurotoxicity was examined using primary cultured rat cortical neurons in the present study. Exposure of c u l t u r e d n e u r o n s t o 1 0 0 µ M H 2 O 2 caused a significant neuronal death as assessed by a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. The addition of CS, over a concentration range of 10 to 50 ㎍ / ㎖ , concentration-dependently prevented the H 2 O 2 -induced neuronal apoptotic death. CS (50 ㎍ / ㎖ ) significantly inhibited H 2 O 2 -induced elevation of the cytosolic Ca 2+ concentration ([Ca 2+ ] c ), which was measured by a fluorescent dye, Fluo-4 AM. CS (30 and 50 ㎍ / ㎖ ) inhibited glutamate release and generation of reactive oxygen species (ROS) induced by 1 0 0 µ M H 2 O 2 . These results suggest that CS may mitigate the H 2 O 2 -induced neurotoxiciy by interfering with the increase of [Ca 2+ ] c , and then inhibiting glutamate release and generation of ROS in cultured neurons.

Abstract

ABSTRACT : Dried leaves from Cedrela sinensis A. Juss. (CS), have been observed to possess various pharmacological activity and contain various antioxidant constituents. The protective effect of ethanol extract of CS on hydrogen peroxide (H2O2)-induced neurotoxicity was examined using primary cultured rat cortical neurons in the present study. Exposure of cultured neurons to 100 μM H2O2 caused a significant neuronal death as assessed by a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl- tetrazolium bromide (MTT) assay and Hoechst 33342 staining. The addition of CS, over a concentration range of 10 to 50㎍/㎖, concentration-dependently prevented the H2O2-induced neuronal apoptotic death. CS (50㎍/㎖) significantly inhibited H2O2-induced elevation of the cytosolic Ca2+ concentration ([Ca2+]c), which was measured by a fluorescent dye, Fluo-4 AM. CS (30 and 50㎍/㎖) inhibited glutamate release and generation of reactive oxygen species (ROS) induced by 100 μM H2O2. These results suggest that CS may mitigate the H2O2-induced neurotoxiciy by interfering with the increase of [Ca2+]c, and then inhibiting glutamate release and generation of ROS in cultured neurons.

참고문헌 (25)

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관련 논문AI추천

Neuroprotective Effect of Korean Mistletoe Extract against DamageInduced by Hydrogen Peroxide in Cultured Rat Cortical Neurons성연희, Ju Hyun Lee한국약용작물학회 · 한국약용작물학회지2007 · 제15권 제2호Protective Effect of Vitis amurensis Stems and Leaves Extract on Hydrogen Peroxide-induced Oxidative Neuronal Cell Damage in Cultured Neurons김주연, 반주연, 배기환, 성연희, 송경식, 주현수한국약용작물학회 · 한국약용작물학회지2009 · 제17권 제1호Methanol Extract of Paeonia Japonica Root Protects Cultured Rat CorticalNeurons Against Oxidative Damage Induced by Hydrogen Peroxide성연희한국약용작물학회 · 한국약용작물학회지2006 · 제14권 제2호Glutamate로 유도된 C6 glial 세포 자멸사에 대한 淸心蓮子飮의 보호효과고석재, 권오상, 신선호, 신용진, 안민섭, 이선아, 장원석, 하예진대한한방내과학회 · 대한한방내과학회지2010 · 제31권 제1호Protective Effect of Sanguisorba officinalis L. Root onAmyloid β Protein (25-35)-induced Neuronal CellDamage in Cultured Rat Cortical Neuron배기환, 성연희, 송경식, Ju Yeon Ban, Soon Ock Cho, So Young Jeon한국약용작물학회 · 한국약용작물학회지2005 · 제13권 제5호Moutan Cortex Extract Inhibits Amyloid β Protein (25-35)-induced Neurotoxicity in Cultured Rat Cortical Neurons김주연, 반주연, 성연희, 송경식, 주현수한국약용작물학회 · 한국약용작물학회지2008 · 제16권 제6호

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