복숭아 품종별 핵과 종자의 항염증 및 항산화 효과간의 상관관계
In vitro correlation between anti-inflammatory and anti-oxidant effects of stone and seed of peaches cultivars
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Peach seeds contain a large amount of phenolic components and exhibit excellent physiological effects in various diseases. We examined the antioxidant effects of stone and seed of three peach cultivars (Miwhang, MH; Kanoiwa hakuto, KH; and Cheonhong, CH) by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, ferric reducing activity of plasma (FRAP) assay, and cupric ion reducing antioxidant capacity (CUPRAC) reduction. The results showed that the stone extracts of CH had higher levels of total phenols and flavonoids than those of the other cultivars do, and the stone extracts of KH and CH have the potential to reduce DPPH, FRAP, and CUPRAC activities. In addition, we found that KH, MH, and CH stone extracts decreased nitric oxide generation in RAW 264.7 and BV2 cells. The total phenol and flavonoid contents had no significant correlation with anti-oxidant activities. On the other hand, the anti-inflammatory activity had a low linear correlation with anti-oxidant activities and total phenol and flavonoid contents. The present results suggest that the correlation between antioxidant and anti-inflammatory effects of stone and seed, and the appropriate combination of the stone and seed extracts could be used as an anti-inflammatory treatment and prevention material, respectively.
Abstract
Peach seeds contain a large amount of phenolic components and exhibit excellent physiological effects in various diseases. We examined the antioxidant effects of stone and seed of three peach cultivars (Miwhang, MH; Kanoiwa hakuto, KH; and Cheonhong, CH) by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, ferric reducing activity of plasma (FRAP) assay, and cupric ion reducing antioxidant capacity (CUPRAC) reduction. The results showed that the stone extracts of CH had higher levels of total phenols and flavonoids than those of the other cultivars do, and the stone extracts of KH and CH have the potential to reduce DPPH, FRAP, and CUPRAC activities. In addition, we found that KH, MH, and CH stone extracts decreased nitric oxide generation in RAW 264.7 and BV2 cells. The total phenol and flavonoid contents had no significant correlation with anti-oxidant activities. On the other hand, the anti-inflammatory activity had a low linear correlation with anti-oxidant activities and total phenol and flavonoid contents. The present results suggest that the correlation between antioxidant and anti-inflammatory effects of stone and seed, and the appropriate combination of the stone and seed extracts could be used as an anti-inflammatory treatment and prevention material, respectively.
참고문헌 (27)
- 지은정, "유자피를 이용한 단일 침출차의 항산화성 및 품질 특성", 한국식품조리과학회지, 24(4), pp.460~465 (2008), 한국식품조리과학회
- Ho CT, "Antioxidative effect of polyphenol extract prepared from various Chinese teas", Prev Med, 21, pp.520~525 (1992)
- 유수정, "살구 추출물의 항산화성, 항돌연변이성 및 세포독성 효과", Food Science and Preservation, 14(2), pp.220~225 (2007), 한국식품저장유통학회
- 황인욱, "포도씨의 proanthocyanidin 함량 및 항산화 활성", Food Science and Preservation, 15(6), pp.859~863 (2008), 한국식품저장유통학회
- Lee DS, "Studies on the chemical composition of major fruits in Korea:On non-volatile organic acid and sugar contents of apricot (maesil), peach, grape, apple and pear and its seasonal variation", Korean J Food Sci Technol, 4, pp.134~139 (1972)
- 차배천, "야생복숭아 나무 추출물의 항산화 및 항염증 효과", 한국약용작물학회지, 12(4), pp.289~294 (2004), 한국약용작물학회
- 이경행, "열풍처리에 의한 복숭아의 성분 및 항산화 활성 변화", 한국식품영양학회지, 27(2), pp.219~224 (2014), 한국식품영양학회
- Studies on functions of Prunus persica to develop as the functional food, "Park KK", Rural Development Administration, 18~26, pp.RDA SE0000106586 (2008)
- Yun IH, "The effect of Persicae Semen on the artherosclerosis in rabbit", Korean J Herbol, 11, pp.79~79 (1996)
- Lee JY, "Inhibition of enzymatic browning of apple juices by benzoic acid isolated from peach (Prunus persica Batsch) seeds", Korean J Postharvest Sci Technol, 7, pp.103~107 (2000)
- Kim JK, "Protective effect of peach kernel extracts on radiation-induced DNA damage in human blood lymphocytes in the comet assay", 1999 spring meeting of the Korean Nuclear Society (1999)
- Folin O, "On phosphotungsticphosphomolybdic compounds as color reagents", J Biol Chem, 12, pp.239~243 (1912)
- Zhishen J, "The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals", Food Chem, 64, pp.555~559 (1999)
- Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Benzie IF, "The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay", Anal Biochem, 239, pp.70~76 (1996)
- Apak R, "Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method", J Agric Food Chem, 52, pp.7970~7981 (2004)
- Hammerstone JF, "Procyanidin content and variation in some commonly consumed foods", J Nutr, 130, pp.2086~2092 (2000)
- 김영진, "식물성 폴리페놀의 항산화성 및 열안정성 평가", 생체재료학회지, 13(1), pp.30~36 (2009), 한국생체재료학회
- Bonina F, "Flavonoids as potential protective agents against photo-oxidative skin damage", Int J Pharm, 145, pp.87~94 (1996)
- Di Carlo G, "Flavonoids: old and new aspects of a class of natural therapeutic drugs", Life Sci, 65, pp.337~353 (1999)
- Chrzczanowicz J, "Simple method for determining human serum 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging activity-possible application in clinical studies on dietary antioxidants", Clinic Chem Lab Med, 46, pp.342~349 (2008)
- Chen S, "Evaluation of garlic cultivars for polyphenolic content and antioxidant properties", PLoS One, 8, pp.e79730~ (2013)
- Kyung Mi Yoo, "Evaluation of Different Methods of Antioxidant Measurement", Food Science and Biotechnology, 16(2), pp.177~182 (2007), 한국식품과학회
- Ozyurek M, "Hydroxyl radical scavenging assay of phenolics and flavonoids with a modified cupric reducing antioxidant capacity (CUPRAC) method using catalase for hydrogen peroxide degradation", Anal Chim Acta, 616, pp.196~206 (2008)
- 김태연, "LPS로 유도된 RAW 264.7 cell의 염증반응에서 MAPK 조절에 의한 加減當歸飮子의 항염증 효과", 대한한방내과학회지, 34(4), pp.339~348 (2013), 대한한방내과학회
- Kreutzberg GW, "Microglia: a sensor for pathological events in the CNS", Trends neurosci, 19, pp.312~318 (1996)
- Cheong MH, "Anti-inflammatory effects of Polygala tenuifolia root through inhibition of NF-<TEX>${\kappa}B$<", TEX> activation in lipopolysaccharide-induced BV2 microglial cells, 2011, pp.137, 1402~1408