Focal adhesion linker proteins expression of fibroblast related to adhesion in response to different transmucosal abutment surfaces
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
PURPOSE. To evaluate adherence of human gingival fibroblasts (HGFs) to transmucosal abutment of dental implant with different surface conditions with time and to investigate the roles of focal adhesion linker proteins (FALPs) involved in HGFs adhesion to abutment surfaces. MATERIALS AND METHODS. Morphologies of cultured HGFs on titanium and ceramic discs with different surface were observed by scanning electron microscopy. Biocompatibility and focal adhesion were evaluated by ultrasonic wave application and cell viability assay. FALPs expression levels were assessed by RT-PCR and western blot. RESULTS. There seemed to be little difference in biocompatibility and adhesion strength of HGFs depending on the surface conditions and materials. In all experimental groups, the number of cells remaining on the disc surface after ultrasonic wave application increased more than 2 times at 3 days after seeding compared to 1-day cultured cells and this continued until 7 days of culture. FALPs expression levels, especially of vinculin and paxillin, also increased in 5-day cultured cells compared to 1-day cultured fibroblasts on the disc surface. CONCLUSION. These results might suggest that the strength of adhesion of fibroblasts to transmucosal abutment surfaces increases with time and it seemed to be related to expressions of FALPs.
Abstract
PURPOSE. To evaluate adherence of human gingival fibroblasts (HGFs) to transmucosal abutment of dental implant with different surface conditions with time and to investigate the roles of focal adhesion linker proteins (FALPs) involved in HGFs adhesion to abutment surfaces. MATERIALS AND METHODS. Morphologies of cultured HGFs on titanium and ceramic discs with different surface were observed by scanning electron microscopy. Biocompatibility and focal adhesion were evaluated by ultrasonic wave application and cell viability assay. FALPs expression levels were assessed by RT-PCR and western blot. RESULTS. There seemed to be little difference in biocompatibility and adhesion strength of HGFs depending on the surface conditions and materials. In all experimental groups, the number of cells remaining on the disc surface after ultrasonic wave application increased more than 2 times at 3 days after seeding compared to 1-day cultured cells and this continued until 7 days of culture. FALPs expression levels, especially of vinculin and paxillin, also increased in 5-day cultured cells compared to 1-day cultured fibroblasts on the disc surface. CONCLUSION. These results might suggest that the strength of adhesion of fibroblasts to transmucosal abutment surfaces increases with time and it seemed to be related to expressions of FALPs.
참고문헌 (39)
- Palma-Carrio C, "Irritative and sensory disturbances in oral implantology. Literature review", Med Oral Patol Oral Cir Bucal, 16, pp.1043~1046 (2011)
- Berglundh T, "The soft tissue barrier at implants and teeth", Clin Oral Implants Res, 2, pp.81~90 (1991)
- Listgarten MA, "Light and transmission electron microscopy of the intact interfaces between non-submerged titaniumcoated epoxy resin implants and bone or gingiva", J Dent Res, 71, pp.364~371 (1992)
- Berglundh T, "Dimension of the peri-implant mucosa: Biological width revisited", J Clin Periodontal, 23, pp.971~973 (1996)
- Ambrose EJ, "The movements of fibrocytes", Exp Cell Res, 8, pp.54~73 (1961)
- Burridge K, "Focal adhesions:transmembrane junctions between the extracellular matrix and the cytoskeleton", Annu Rev Cell Biol, 4, pp.487~525 (1988)
- Hynes RO, "Integrins: bidirectional, allosteric signaling machines", Cell, 110, pp.673~687 (2002)
- Owen GR, "Focal adhesion quantification - a new assay of material biocompatibility? Review", Eur Cell Mater, 9, pp.85~96 (2005)
- An N, "Initial attachment, subsequent cell proliferation", viability and gene expression of epithelial cells related to attachment and wound healing in response to different titanium surfaces, 2012, pp.28, 1207~1214
- Anselme K, "Qualitative and quantitative study of human osteoblast adhesion on materials with various surface roughnesses", J Biomed Mater Res, 49, pp.155~166 (2000)
- Boyan BD, "Mechanisms involved in osteoblast response to implant surface morphology", Annual Rev Mater Res, 31, pp.357~371 (2001)
- Garrod DR, "Cell to cell and cell to matrix adhesion", BMJ, 306, pp.703~705 (1993)
- Giancotti FG, "Integrin signaling", Science, 285, pp.1028~1032 (1999)
- Raisanen L, "Expression of cell adhesion complexes in epithelial cells seeded on biomaterial surfaces", J Biomed Mater Res, 49, pp.79~87 (2000)
- Biggs MJ, "Regulation of implant surface cell adhesion: characterization and quantification of S-phase primary osteoblast adhesions on biomimetic nanoscale substrates", J Orthop Res, 25, pp.273~282 (2007)
- Arvidson K, "Histological characteristics of peri-implant mucosa around Branemark and single-crystal sapphire implants", Clin Oral Implants Res, 7, pp.1~10 (1996)
- Abrahamsson I, "The mucosal attachment at different abutments. An experimental study in dogs", J Clin Periodontol, 25, pp.721~727 (1998)
- Craig RG, "Cytotoxicity of experimental casting alloys evaluated by cell culture tests", J Dent Res, 69, pp.1539~1542 (1990)
- Li D, "Biomechanical comparison of the sandblasted and acid-etched and the machined and acid-etched titanium surface for dental implants", J Biomed Mater Res, 60, pp.325~332 (2002)
- Martin JY, "Effect of titanium surface roughness on proliferation, differentiation,and protein synthesis of human osteoblast-like cells", J Biomed Mater Res, 29, pp.389~401 (1995)
- Kieswetter K, "Surface roughness modulates the local production of growth factors and cytokines by osteoblast-like MG-63 cells", J Biomed Mater Res, 32, pp.55~63 (1996)
- Batzer R, "Prostaglandins mediate the effects of titanium surface roughness on MG63 osteoblast-like cells and alter cell responsiveness to 1 alpha,25-(OH)2D3", J Biomed Mater Res, 41, pp.489~496 (1998)
- Lossdorfer S, "Microrough implant surface topographies increase osteogenesis by reducing osteoclast formation and activity", J Biomed Mater Res A, 70, pp.361~369 (2004)
- Kononen M, "Effect of surface processing on the attachment, orientation, and proliferation of human gingival fibroblasts on titanium", J Biomed Mater Res, 26, pp.1325~1341 (1992)
- Grossner-Schreiber B, "Focal adhesion contact formation by fibroblasts cultured on surface-modified dental implants:an in vitro study", Clin Oral Implants Res, 17, pp.736~745 (2006)
- Park J, "Nanosize and vitality: TiO2 nanotube diameter directs cell fate", Nano Lett, 7, pp.1686~1691 (2007)
- Park J, "TiO2 nanotube surfaces: 15 nm-an optimal length scale of surface topography for cell adhesion and differentiation.", Small, 5, pp.666~671 (2009)
- Meredith DO, "Human fibroblast reactions to standard and electropolished titanium and Ti-6Al-7Nb, and electropolished stainless steel", J Biomed Mater Res A, 75, pp.541~555 (2005)
- Derhami K, "Quantifying the adherence of fibroblasts to titanium and its enhancement by substrate-attached material", J Biomed Mater Res, 52, pp.315~322 (2000)
- Groessner-Schreiber B, "Fibroblast growth on surfacemodified dental implants: an in vitro study", J Biomed Mater Res A, 64, pp.591~599 (2003)
- Reyes CD, "A centrifugation cell adhesion assay for high-throughput screening of biomaterial surfaces", J Biomed Mater Res A, 67, pp.328~333 (2003)
- Hunter A, "Attachment and proliferation of osteoblasts and fibroblasts on biomaterials for orthopaedic use", Biomaterials, 16, pp.287~295 (1995)
- Richards RG, "Immunogold labelling of fibroblast focal adhesion sites visualised in fixed material using scanning electron microscopy, and living, using internal reflection microscopy", Cell Biol Int, 25, pp.1237~1249 (2001)
- Grover A, "An adhesion-defective variant of F9 embryonal carcinoma cells fails to differentiate into visceral endoderm", Dev Biol, 120, pp.1~11 (1987)
- Rodriguez Fernandez JL, "Overexpression of vinculin suppresses cell motility in BALB", c 3T3 cells, 1992, pp.22, 127~134
- Rodriguez Fernandez JL, "Suppression of vinculin expression by antisense transfection confers changes in cell morphology, motility, and anchoragedependent growth of 3T3 cells", J Cell Biol, 122, pp.1285~1294 (1993)
- Alenghat FJ, "Analysis of cell mechanics in single vinculin-deficient cells using a magnetic tweezer", Biochem Biophys Res Commun, 277, pp.93~99 (2000)
- Turner CE, "Paxillin and focal adhesion signalling", Nat Cell Biol, 2, pp.231~236 (2000)
- Brown MC, "Paxillin: adapting to change", Physiol Rev, 84, pp.1315~1339 (2004)