Cellular attachment and gene expression of osteoblast-like cells on zirconia ceramic surfaces
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STATEMENT OF PROBLEM: Zirconium oxide can be a substitute to titanium as implant materials to solve the esthetic problems of dark color in the gingival portion of implant restorations. PURPOSE: This study was performed to define attachment and growth behavior of osteoblast- like cells cultured on grooved surfaces of zirconium oxide and evaluate the genetic effect of zirconium oxide surfaces using the reverse transcriptase-polymerase chain reaction (RT-PCR). MATERIAL AND METHODS: MC3T3-E1 cells were cultured on (1) commercially pure titanium discs with smooth surface (T group), (2) yttrium-stabilized tetragonal zirconia polycrystal (Y-TZP) with machined surface (ZS group), and (3) Y-TZP with 100μm grooves (ZG group). Cell proliferation activity was evaluated through MTT assay and cell morphology was examined by SEM. The mRNA expression of Runx2, alkaline phosphatase, osteocalcin, TGF-β1, IGF-1, G3PDH in E1 cells were evaluated by RT-PCR. RESULTS: From the MTT assay, after 48 hours of adhesion of MC3T3-E1 cells, the mean optical density value of T group and ZG group significantly increased compared to the ZS group. SEM images of osteoblast-like cells showed that significantly more cells were observed to attach to the grooves and appeared to follow the direction of the grooves. After 24 hours of cell adhesion, more spreading and flattening of cells with active filopodia formation occurred. Results of RT-PCR suggest that T group, ZS group, and ZG group showed comparable osteoblast-specific gene expression after 24 hours of cell incubation. CONCLUSION: Surface topography and material of implants can play an important role in expression of osteoblast phenotype markers. Zirconia ceramic showed comparable biological responses of osteoblast-like cells with titanium during a short-time cell culture period. Also, grooves influence cell spreading and guide the cells to be aligned within surface grooves.
Abstract
STATEMENT OF PROBLEM: Zirconium oxide can be a substitute to titanium as implant materials to solve the esthetic problems of dark color in the gingival portion of implant restorations. PURPOSE: This study was performed to define attachment and growth behavior of osteoblast- like cells cultured on grooved surfaces of zirconium oxide and evaluate the genetic effect of zirconium oxide surfaces using the reverse transcriptase-polymerase chain reaction (RT-PCR). MATERIAL AND METHODS: MC3T3-E1 cells were cultured on (1) commercially pure titanium discs with smooth surface (T group), (2) yttrium-stabilized tetragonal zirconia polycrystal (Y-TZP) with machined surface (ZS group), and (3) Y-TZP with 100μm grooves (ZG group). Cell proliferation activity was evaluated through MTT assay and cell morphology was examined by SEM. The mRNA expression of Runx2, alkaline phosphatase, osteocalcin, TGF-β1, IGF-1, G3PDH in E1 cells were evaluated by RT-PCR. RESULTS: From the MTT assay, after 48 hours of adhesion of MC3T3-E1 cells, the mean optical density value of T group and ZG group significantly increased compared to the ZS group. SEM images of osteoblast-like cells showed that significantly more cells were observed to attach to the grooves and appeared to follow the direction of the grooves. After 24 hours of cell adhesion, more spreading and flattening of cells with active filopodia formation occurred. Results of RT-PCR suggest that T group, ZS group, and ZG group showed comparable osteoblast-specific gene expression after 24 hours of cell incubation. CONCLUSION: Surface topography and material of implants can play an important role in expression of osteoblast phenotype markers. Zirconia ceramic showed comparable biological responses of osteoblast-like cells with titanium during a short-time cell culture period. Also, grooves influence cell spreading and guide the cells to be aligned within surface grooves.
참고문헌 (48)
- Kasemo B, "Biomaterial and implant surfaces: A surface science approach", Int J Oral Maxillofac Implants, 3, pp.247~259 (1988)
- Carlsson L, "Osseointegration of titanium implants", Acta Orthop Scand, 57, pp.285~289 (1986)
- Anselme K, "Effect of grooved titanium substratum on human osteoblastic cell growth", J Biomed Mater Res, 60, pp.529~540 (2002)
- Li LH, "Improved biological performance of Ti implants due to surface modification by micro-arc oxidation", Biomaterials, 25, pp.2867~2875 (2004)
- Brunette DM, "Effects of surface topography of implant materials on cell behavior in vitro and in vivo. In: Hoch HC, editor. Nanofabrication and biosystems" (1996), Cambridge, UK: Cambridge University Press
- Mustafa K, "Attachment and proliferation of human oral fibroblasts to titanium surfaces blasted with TiO2 particles. A scanning electron microscopic and histomorphometric analysis", Clin Oral Impl Res, 9, pp.195~207 (1998)
- Yoshinari M, "Effects of multigrooved surfaces on fibroblasts behavior", J Biomed Mater Res, 65A, pp.359~368 (2003)
- Anselme K, "The relative influence of the topography and chemistry of Ti Al6V4 surfaces on osteoblastic cell behavior", Biomater, 21, pp.1567~1577 (2000)
- Bachle M, "Behavior of CAL72 osteoblast-like cells cultured on zirconia ceramics with different surface topographies", Clin Oral Impl Res, 18, pp.53~59 (2007)
- Heydecke G, "Optimal esthetics in single-tooth replacement with the Re-Implant system: A case report", Int J Prosthodont, 12, pp.184~189 (1999)
- Piconi C, "Zirconia as a ceramic biomaterial", Biomaterials, 20, pp.1~25 (1999)
- Garvie RC, "Ceramic steel?", Nature, 258, pp.703~704 (1975)
- Manicone PF, "An overview of zirconia ceramics: Basic properties and clinical applications", J Dent, 35, pp.819~826 (2007)
- Ichikawa Y, "Tissue compatiblity and stability of a new zirconia ceramic in vivo", J Prosthet Dent, 68, pp.322~326 (1992)
- Josset Y, "In vitro reactions of human osteoblasts in culture with zirconia and alumina ceramics", J Biomed Mater Res, 47, pp.481~493 (1999)
- Scarano A, "Bacterial adhesion on commercially pure titanium and zirconium oxide disks: and in vivo human study", J Periodontol, 75, pp.292~296 (2004)
- Rimondini L, "Bacterial colonization of zirconia ceramic surfaces: an invitro and in vivo study", Int J Oral Maxillofac Implants, 17, pp.793~798 (2002)
- Degidi M, "Inflammatory infiltrate, microvessel density, nitric oxide synthase expression, vascular endothelial growth factor expression, and proliferative activity in peri-implant soft tissues around titanium and zirconium oxide healing caps", J Periodontol, 77, pp.73~80 (2006)
- Carinci F, "Zirconium oxide: analysis of MG63 osteoblast-like cell response by means of microarray technology", Biomaterials, 25, pp.215~228 (2004)
- Lalor PA, "Sensitivity to titanium. A cause of implant failure?", The Journal of Bone and Joint Surgery. British volume, 73, pp.25~28 (1991)
- Yamauchi R, "Pacemaker dermatitis from titanium", Contact Dermatitis, 42, pp.52~53 (2000)
- Albrektsson T, "Interface histology of unloaded and early loaded partially stabilized zirconia endosseous implant in initial bone healing", J Prosthet Dent, 69, pp.599~604 (1993)
- Akagawa Y, "Interface histology of unloaded and early loaded partially stabilized zirconia endosseous implants in initial bone healing", J Prosthet Dent, 69, pp.599~604 (1993)
- Akagawa Y, "Comparison between free-standing and tooth-connected partially stabilized zirconia implants after two years’function in monkeys: A clinical and histologic study", J Prosthet Dent, 80, pp.551~558 (1998)
- Kohal RJ, "Loaded custommade zirconia and titanium implants show similar osseointegration", J Periodontol, 75, pp.1262~1268 (2004)
- Kohal RJ, "Custom-made root analogue titanium implants placed into extraction sockets. An experimental study in monkeys", Clin Oral Implants Res, 8, pp.386~392 (1997)
- Sennerby L, "Bone tissue responses to surface-modified zirconia implants: a histomorphometric and removal torque study in the rabbit", Clin Implant Dent Relat Res, 7(1), pp.s13~s20 (2005)
- Ko HC, "Initial osteoblast-like cell response to pure titanium and zirconia", alumina ceramics, 2007, pp.23, 1349~1355
- Weiss P, "Experiments on cell and axon orientation in vitro, the role of colloidal exudates in tissue organization", J Exp Zool, 100, pp.353~386 (1945)
- Matsuzaka K, "The attachment and growth behavior of osteoblast-like cells on microtextured surfaces", Biomater, 24, pp.2711~2719 (2003)
- Anselme K, "Osteoblast adhesion on biomaterials", Biomater, 21, pp.667~681 (2000)
- Mustafa K, "Determining optimal surface roughness of TiO2 blasted titanium implant material for attachment, proliferation and differentiation of cells derived from human mandibular alveolar bone", Clin Oral Imp Res, 12, pp.515~525 (2001)
- Mustafa K, "The influence of surface topography of ceramic abutments on the attachment and proliferation of human oral fibroblasts", Biomater, 26, pp.373~381 (2005)
- Hormia M, "Immunolocalization of proteins specific for adherents junctions in human gingival epithelial cells grown on differently processed titanium surfaces", J Perio Res, 26, pp.491~497 (1991)
- Guy SC, "In vitro study of attachment of human gingival fibroblasts to endosseous implant materials", J Periodontol, 64, pp.542~546 (1993)
- Cochran DL, "Attachment and growth of periodontal cells on smooth and rough titanium", Int J Oral Maxillofac Implants, 3, pp.21~24 (1988)
- Bowers KT, "Optimization of surface micromorphology for enhanced osteoblast responses in vitro", Int J Oral Maxillofac Implants, 7, pp.302~310 (1992)
- Gotfredsen K, "Histomorphometric and removal torque analysis for TiO2-blasted titanium implants. An experimental study on dogs", Clin Oral Imp Res, 2, pp.77~84 (1992)
- Ask M, "Preparation and surface spectroscopic characterization of oxide films on Ti6Al4V", Appl Surf Sci, 35, pp.283~301 (1988)
- Hunter A, "Attachments and proliferation of osteoblasts and fibroblasts on biomaterials for orthopedic use", Biomater, 16, pp.287~295 (1995)
- Ismail M, "The influence of surface chemistry and topography on the contact guidance of MG63 osteoblast cells", J Mater Sci Mater Med, 18, pp.705~714 (2007)
- Marinucci L, "Effect of surface roughness on human osteoblast proliferation and gene expression in vitro", Int J Oral Maxillofac Implants, 21, pp.719~725 (2006)
- Martin JY, "Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63)", J Biomed Materi Res, 29, pp.389~401 (1995)
- Zitzmann NU, "Soft tissue reactions of plaque formation at implant abutments with different surface topography. An experimental study in dogs", J Clin Periodontol, 29, pp.456~461 (2002)
- Oliva J, "One-year follow-up of first consecutive 100 zirconia dental implants in humans: a comparison of 2 different rough surfaces", Int J Oral Maxillofac Implants, 22, pp.430~435 (2007)
- Sollazzo V, "Zirconium oxide coating improves implant osseointegration in vivo", Dental Materials, 17 (2007)
- Kohal RJ, "Three-dimensional computerized stress analysis of commercially pure titanium and yttriumpartially stabilized zirconia implants", Int J Prosthodont, 15, pp.189~194 (2002)
- Gahert M, "Biomechanical and histomorphometric comparison between zirconia implants with varying surface textures and a titanium implant in the maxilla of miniature pigs", Clin Oral Imp Res, 18, pp.662~668 (2007)