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항산균 배양에서 BACTEC Mycobacteria Growth Indicator Tube 960 System과 Ogawa 배지 병용의 평가

Evaluation of Combination of BACTEC Mycobacteria Growth Indicator Tube 960 System and Ogawa Media for Mycobacterial Culture

임지훈|김성원|김미나|심태선|성흥섭|윤남섭|배은신
대한진단검사의학회 (Korean Society For Laboratory Medicine)|2008.08|Annals of Laboratory Medicine|제28권 제4호|pp. 299-306

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초록

Abstract

Background : The combined use of liquid media and solid media is recommended for mycobacterial culture. We evaluated diagnostic performance of combination of BACTEC Mycobacteria Growth Indicator Tube (MGIT; Becton Dickinson, USA) and 2% Ogawa media (Korean Institute of Tuberculosis, Korea) for recovery of mycobacteria. Methods : In September 2007, 1,764 specimens from 1,059 patients were cultured with MGIT and Ogawa. Acid fast bacilli (AFB) smear was fluorochrome-stained. The isolates were identified into Mycobacterium tuberculosis (MTB) and nontuberculous mycobacteria (NTM) with PCR using Seeplex TB Detection Kit (Seegene, Korea). Recovery rate, time to detection (TTD), contamination rate, mixed growth rate and species distribution were analyzed. Results : Two hundred thirty-five specimens (13.3%) from 165 patients (15.6%) were positive for mycobacterial culture. Recovery rates of mycobacteria from the group using both media, MGIT only, and Ogawa only were 13.3%, 12.1%, and 7.8%, respectively. While MGIT recovered 98.9% of MTB and 79.7% of NTM, Ogawa recovered 65.9% of MTB and 54.1% of NTM. TTDs of total mycobacteria/MTB/NTM in MGIT and Ogawa were 10.6/11.4/9.7 days and 31/29/33 days, respectively. MGIT TTDs of total mycobacteria/MTB/NTM from AFB-positive specimens were significantly shorter than those of AFB-negative specimens; 8.2/9.5/4.4 days vs 11.6/12.7/10.7 days. Contamination and mixed growth rate of MGIT were 9.6% and 3.7%. Primary culture of Ogawa recovered 1 MTB and 1 NTM among the 170 MGIT-contaminated specimens and 38 mycobacteria among 66 specimens that showed mixed cultures of MGIT. Conclusions : MGIT warrants sensitive and rapid isolation of mycobacteria. However, the combination of MGIT and Ogawa is more desirable to recover mycobacteria in the case of contaminations or mixed cultures. (Korean J Lab Med 2008;28:299-306)

참고문헌 (28)

  1. 이효원, "면역적격자에서 비결핵마이코박테리아의 폐감염", Tuberculosis and Respiratory Diseases, 53(2), pp.173~182 (2002), 대한결핵및호흡기학회
  2. Koh WJ, "Nontuberculous mycobacterial pulmonary diseases in imunocompetent patients", Korean J Radiol, 3, pp.145~157 (2002)
  3. 고원중, "항산균 도말양성 객담에서 비결핵성 마이코박테리아의 분리 비율", Tuberculosis and Respiratory Diseases, 54(1), pp.22~32 (2003), 대한결핵및호흡기학회
  4. Bird BR, "Changing practices in mycobacteriology: a follow-up survey of state and territorial public health laboratories", J Clin Microbiol, 34, pp.554~549 (1996)
  5. Global Tuberculosis Control Surveillance, Planning, Financing, WHO Report 2007, "World Health Organization", World Health Organization, 376~, pp.WHO (2007), HTM[DOI]
  6. Pfyffer GE, "Mycobacterium: general characteristics, laboratory detection, and staining procedures in: Manual of Clinical Microbiology", pp.543~572 (2007), ASM press
  7. Kim MN, "Mycobacterial testing in hospital laboratories in Korea: results of a survey of 40 university or tertiary-care hospitals", Korean J Clin Pathol, 19, pp.86~91 (1999)
  8. Chang CH, "Survey on changes in mycobacterial testing practices in Korean laboratories", Korean J Clin Microbiol, 4, pp.108~114 (2001)
  9. Choi YM, "Evaluation of the BACTEC MGIT 960 system for the recovery of mycobacteria", Korean J Clin Pathol, 20, pp.56~61 (2000)
  10. Yi JY, "Detection of Mycobacterium tuberculosis using BACTEC Mycobacteria Growth Indicator Tube (MGIT) 960 system-comparison with BACTEC 460 TB system and ogawa media", Korean J Clin Pathol, 20, pp.384~391 (2000)
  11. 정의석, "Mycobacterium growth indicator tube와 오가와 배지에서의 mycobacteria 배양성적에 대한 비교", 대한임상미생물학회지, 7(2)통권 : 13, pp.135~138 (2004), 대한임상미생물학회
  12. Hanna BA, "Rusch-Gerdes S. Multicenter evaluation of the BACTEC MGIT 960 system for recovery of mycobacteria", J Clin Microbiol, 37, pp.748~752 (1999)
  13. Kanchana MV, "Evaluation of the BACTEC MGIT 960 system for the recovery of mycobacteria", Diagn Microbiol Infect Dis, 37, pp.31~36 (2000)
  14. Badak FZ, "Comparison of mycobacteria growth indicator tube with BACTEC 460 for detection and recovery of mycobacteria from clinical specimens", J Clin Microbiol, 34, pp.2236~2239 (1996)
  15. Tortoli E, "Use of BACTEC MGIT 960 for recovery of mycobacteria from clinical specimens: multicenter study", J Clin Microbiol, 37, pp.3578~3582 (1999)
  16. Leitritz L, "Evaluation of BACTEC MGIT 960 and BACTEC 460TB systems for recovery of mycobacteria from clinical specimens of a university hospital with low incidence of tuberculosis", J Clin Microbiol, 39, pp.3764~3767 (2001)
  17. Cruciani M, "Meta-analysis of BACTEC MGIT 960 and BACTEC 460 TB, with or without solid media, for detection of mycobacteria", J Clin Microbiol, 42, pp.2321~2326 (2004)
  18. Ito A, "The role of simultaneous combination culture with solid and liquid media for diagnosis of pulmonary tuberculosis", Kekkaku, 81, pp.401~405 (2006)
  19. Saitoh H, "Comparative evaluation of BACTEC MGIT 960 system with MB", BacT and egg-based media for recovery of mycobaceria, 2000, pp.11, 19~26
  20. Falkinham JO 3rd., "Epidemiology of infection by nontuberculous mycobacteria", Clin Microbiol Rev, 9, pp.177~215 (1996)
  21. 임재준, "비결핵성 마이코박테리아 폐질환의 진단과 치료", 대한의사협회지, 48(6)통권 : 545, pp.563~570 (2005), 대한의사협회
  22. Griffith DE, "An official ATS", IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases, 2007, pp.175, 367~416
  23. Koh WJ, "Clinical significance of nontuberculous mycobacteria isolated from respiratory specimens", Korean J Med, 65, pp.10~21 (2003)
  24. 최순필, "일개 국립결핵병원에서 경험한 비결핵성 마이코박테리아 폐질환의 원인균과 임상상", 결핵 및 호흡기질환, 59(6), pp.606~612 (2005), 대한결핵및호흡기학회
  25. Somoskovi A, "Use of molecular methods to identify the Mycobacterium tuberculosis complex (MTBC) and other mycobacterial species and to detect rifampin resistance in MTBC isolates following growth detection with the BACTEC MGIT 960 system", J Clin Microbiol, 41, pp.2822~2826 (2003)
  26. Diagnosis and treatment of disease caused by nontuberculous mycobacteria, "This official statement of the American Thoracic Society was approved by the Board of Directors", Am J Respir Crit Care Med, 156, pp.S1~S25 (1997), Medical Section of the American Lung Association
  27. American Thoracic Society, "CDC, and Infectious Diseases Society of America. Treatment of tuberculosis", MMWR Recomm Rep, 52, pp.1~77 (2003)
  28. Cornfield DB, "Mycobacterial growth and bacterial contamination in the mycobacteria growth indicator tube and BACTEC 460 culture systems", J Clin Microbiol, 35, pp.2068~2071 (1997)

관련 논문AI추천

결핵균 검출을 위한 고체배지(Ogawa)와 액체배지(MGIT)의 비교현진, 김소정, 김윤호, 이도현, 이현아, 황선철대한임상검사과학회 · 대한임상검사과학회지2001 · 제33권 제2호마이코박테리아 검출을 위하여 MGIT 양성 신호를 보인 세균에오염된 검체 재처리 과정의 효율성 평가방해인, 정해용, 최태윤대한임상검사과학회 · 대한임상검사과학회지2019 · 제51권 제2호항산균 배양에서 BACTEC MGIT 960 System의 유용성 및 MGIT 양성배지에서 결핵균 진단을 위한 TB Ag MPT64 면역발색법의 유용성김선주, 김유은, 김호철, 이민정, 이승준, 이승훈, 이정미, 이종덕, 임수진, 정이영, 조유지, 황영실영남대학교 의과대학 · Journal of Yeungnam Medical Science2012 · 제29권 제2호Rapid Diagnosis of Tuberculosis and Multidrug Resistance Using a MGIT 960 System고원중, 고유상, 박경선, 이남용, Chang-Ki Kim대한진단검사의학회 · Annals of Laboratory Medicine2012 · 제32권 제4호결핵균의 항결핵제 감수성 시험방법에 관한 고찰최철석대한임상검사과학회 · 대한임상검사과학회지1997 · 제29권 제1호항산균 배양 및 동정방법의 변화에 따른 양성률과 보고시간 변화박도심, 이기은, 이영진, 조지현대한진단검사의학회 · Annals of Laboratory Medicine2005 · 제25권 제1호

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