결핵균 검출을 위한 고체배지(Ogawa)와 액체배지(MGIT)의 비교

Abstract

As the world wide concem over prevalence and epidemics of tuberculosis, especially among immunocompromised individuals has mounted, rapid and accurate identification of the causative agents, chemotherapy of the patients and preventive measures appear to be important problems in control of tuberculosis. The study was to evaluate and compare two different TB media for their efficiency in recovery of Mycobacterium tuberculosis testing 400 clinical specimens from 250 patients. The media used were egg based 3% Ogawa TB media and commercially available MGIT 960 system. For the identification of the isolates, AFB staining, multiplex -PCR using type specific genes(IS6110 and hps65) to differentiate between M. tuberculosis and Mycobacteria other than tuberculosis(MOTT), and COBAS Amplicor System was employed as a complete classification of the organisms. A total number of fifty-eight(14.5%) M. tuberculosis strains were isolated from 400 specimens revealing 91.4% and 41.4% of the recovery rates by MGIT and Ogawa, respectively. The average length of time elapsed for the first colony appearance in MGIT and Ogawa were 11.6 days and 32.9 days, and the rates of contamination were 23.5% and 5.7%, respectively. The study showed that the MGIT 960 System was found to be superior in recovery rate of M. tuberculosis, and took shorter time to grow than the Ogawa. However, the higher rate of contamination in MGIT than in Ogawa remains to be problematic. If the problems like the contamination and high cost of the MGIT are solved, the use of MGIT system would be very effective and economic in the identification of the organisms, and consequently efficient measures can be achieved for the control of tuberculosis.