Rapid Diagnosis of Tuberculosis and Multidrug Resistance Using a MGIT 960 System
2
조회
0
다운로드
9
인용
0
참고문헌
키워드
초록
Background: The purpose of this study was to compare the turnaround time for liquid culturing and primary anti-tuberculous drug susceptibility testing (DST) performed using the mycobacteria growth indicator tube (MGIT) 960 system (Becton Dickinson, USA) with that for conventional culturing and DST (by the absolute concentration method) performed using solid culture medium and to determine the concordance rates of DST results obtained using these 2 methods. Methods: In this retrospective study, we compared the turnaround times from receiving the request for mycobacterial culture to reporting the DST results before and after the introduction of the MGIT 960 system. Further, we determined the concordance between DST results for isoniazid and rifampin for Mycobacterium tuberculosis isolates obtained using the MGIT 960 system and the absolute concentration method, which was conducted at the Korean Institute of Tuberculosis. Results: The overall turnaround time for mycobacterial culturing and DST was 27 days for liquid culturing and DST using the MGIT 960 system versus approximately 70 days for culturing on solid medium and DST with the absolute concentration method (P <0.001). There was a good concordance between findings of DST obtained with the 2 methods (97.2%, kappa coefficient=0.855 for rifampin; and 95.6%, kappa coefficient=0.864 for isoniazid),for 1,083 clinical isolates. Conclusions: The automated MGIT 960 system for culturing and DST of M. tuberculosis was successfully introduced in a hospital laboratory setting in Korea with significant shortening of the turnaround time.
Abstract
Background: The purpose of this study was to compare the turnaround time for liquid culturing and primary anti-tuberculous drug susceptibility testing (DST) performed using the mycobacteria growth indicator tube (MGIT) 960 system (Becton Dickinson, USA) with that for conventional culturing and DST (by the absolute concentration method) performed using solid culture medium and to determine the concordance rates of DST results obtained using these 2 methods. Methods: In this retrospective study, we compared the turnaround times from receiving the request for mycobacterial culture to reporting the DST results before and after the introduction of the MGIT 960 system. Further, we determined the concordance between DST results for isoniazid and rifampin for Mycobacterium tuberculosis isolates obtained using the MGIT 960 system and the absolute concentration method, which was conducted at the Korean Institute of Tuberculosis. Results: The overall turnaround time for mycobacterial culturing and DST was 27 days for liquid culturing and DST using the MGIT 960 system versus approximately 70 days for culturing on solid medium and DST with the absolute concentration method (P <0.001). There was a good concordance between findings of DST obtained with the 2 methods (97.2%, kappa coefficient=0.855 for rifampin; and 95.6%, kappa coefficient=0.864 for isoniazid),for 1,083 clinical isolates. Conclusions: The automated MGIT 960 system for culturing and DST of M. tuberculosis was successfully introduced in a hospital laboratory setting in Korea with significant shortening of the turnaround time.
참고문헌 (25)
- Orenstein EW, "Treatment outcomes among patients with multidrug-resistant tuberculosis: systematic review and meta-analysis", Lancet Infect Dis, 9, pp.153~161 (2009)
- Johnston JC, "Treatment outcomes of multidrug-resistant tuberculosis: a systematic review and meta- analysis", PLoS One, 4, pp.e6914~ (2009)
- Kim HR, "Impact of extensive drug resistance on treatment outcomes in non-HIV-infected patients with multidrug-resistant tuberculosis", Clin Infect Dis, 45, pp.1290~1295 (2007)
- Kwon YS, "Treatment outcomes for HIV-uninfected patients with multidrug-resistant and extensively drug-resistant tuberculosis", Clin Infect Dis, 47, pp.496~502 (2008)
- Kim DH, "Treatment outcomes and long-term survival in patients with extensively drug-resistant tuberculosis", Am J Respir Crit Care Med, 178, pp.1075~1082 (2008)
- 전두수, "Treatment Outcome and Mortality among Patients with Multidrug-resistant Tuberculosis in Tuberculosis Hospitals of the Public Sector", Journal of Korean Medical Science, 26(1), pp.33~41 (2011), 대한의학회
- Van Deun A, "Diagnosis of drug-resistant tuberculosis: reliability and rapidity of detection", Int J Tuberc Lung Dis, 14, pp.131~140 (2010)
- O’Grady J, "New and improved diagnostics for detection of drug-resistant pulmonary tuberculosis", Curr Opin Pulm Med, 17, pp.134~141 (2011)
- Kim SJ, "Drug-susceptibility testing in tuberculosis: methods and reliability of results", Eur Respir J, 25, pp.564~569 (2005)
- Wright A, "Epidemiology of antituberculosis drug resistance 2002-07: an updated analysis of the Global Project on Anti-Tuberculosis Drug Resistance Surveillance", Lancet, 373, pp.1861~1873 (2009)
- Schaberg T, "Rapid drug susceptibility testing of Mycobacterium tuberculosis using conventional solid media", Eur Respir J, 8, pp.1688~1693 (1995)
- Muralidhar S, "Evaluation of three methods to determine the antimicrobial susceptibility of Mycobacterium tuberculosis", Indian J Med Res, 120, pp.463~467 (2004)
- Use of liquid TB culture and drug susceptibility testing (DST) in low- and medium-income settings, In Summary report of the Expert Group Meeting on the Use of Liquid Culture Media, "World Health Organization (WHO)", WHO, ~ (2007)
- Bai GH, "Trend of anti-tuberculosis drug resistance in Korea, 1994-2004", Int J Tuberc Lung Dis, 11, pp.571~576 (2007)
- Jae Chol Choi, "Drug Resistance Rates of Mycobacterium tuberculosis at a PrivateReferral Center in Korea", Journal of Korean Medical Science, 22(4), pp.677~681 (2007), 대한의학회
- 배은신, "항산균 배양에서 BACTEC Mycobacteria Growth Indicator Tube 960 System과 Ogawa 배지 병용의 평가", Annals of Laboratory Medicine, 28(4), pp.299~306 (2008), 대한진단검사의학회
- 김병주, "국내 다제내성 결핵의 실태", Tuberculosis and Respiratory Diseases, 60(4), pp.404~411 (2006), 대한결핵및호흡기학회
- 오승환, "Comparison of Anti-mycobacterial Drug Susceptibility Test Results by Institutes and Methods", Annals of Clinical Microbiology, 11(1), pp.43~48 (2008), 대한임상미생물학회
- Han MD, "A study on the drug susceptibility test of multi-drug resistant tuberculosis patients", Korean J Epidemiol, 30, pp.301~308 (2008)
- Blumberg HM, "American Thoracic Society", Centers for Disease Control and Prevention, Am J Respir Crit Care Med, pp.2003, 167[DOI]
- Joon Sung Joh, "The Interval Between Initiation of Anti-tuberculosis Treatment in Patients with Culture-positive Pulmonary Tuberculosis and Receipt of Drug-susceptibility Test Results", Journal of Korean Medical Science, 22(1), pp.26~29 (2007), 대한의학회
- Abe C, "Biological and molecular characteristics of Mycobacterium tuberculosis clinical isolates with low-level resistance to isoniazid in Japan", J Clin Microbiol, 46, pp.2263~2268 (2008)
- Van Deun A, "Mycobacterium tuberculosis strains with highly discordant rifampin susceptibility test results", J Clin Microbiol, 47, pp.3501~3506 (2009)
- Van Deun A, "Diagnosis of drug-resistant tuberculosis: reliability and rapidity of detection", Int J Tuberc Lung Dis, 14, pp.131~140 (2010)
- Bai GH, "Proficiency analysis of drug susceptibility testing by national-level tuberculosis reference laboratories from 1995 to 2003", J Clin Microbiol, 45, pp.3626~3630 (2007)