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Bacillus subtilis AH18의 고추역병 방제능과 SiderophoreAH18의 구조분석

Structural Identification of SiderophoreAH18 from Bacillus subtilis AH18, a Biocontrol agent of Phytophthora Blight Disease in Red-pepper.

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초록

The siderophore (siderophoreAH18) of Bacillus subtilis AH18 was determined to be one of catechol type and purified by using Amberlite XAD-2, Sephadex LH-20 chromatography, and reversed-phase HPLC. The siderophoreAH18 was identified bacillibactin with its structure by GC-MS, 1H-NMR, and 13C-NMR. SiderophoreAH18 (bacillibactin) had been confirmed its molecular weight of 883 and chemical structure of (2,3-dihydroxybenzoate-glycine-threonine)3. Purified siderophoreAH18 showed strong biocontrol ability towards the spore of Phytophthora capsici on PDA and able to effectively suppress (55%) P. capsici causing red-pepper blight in the pot in vivo test.

Abstract

The siderophore (siderophoreAH18) of Bacillus subtilis AH18 was determined to be one of catechol type and purified by using Amberlite XAD-2, Sephadex LH-20 chromatography, and reversed-phase HPLC. The siderophoreAH18 was identified bacillibactin with its structure by GC-MS, 1H-NMR, and 13C-NMR. SiderophoreAH18 (bacillibactin) had been confirmed its molecular weight of 883 and chemical structure of (2,3-dihydroxybenzoate-glycine-threonine)3. Purified siderophoreAH18 showed strong biocontrol ability towards the spore of Phytophthora capsici on PDA and able to effectively suppress (55%) P. capsici causing red-pepper blight in the pot in vivo test.

참고문헌 (45)

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