본문 바로가기
KERD
논문KCI 등재

Multiplex PCR을 이용한 생물방제균 Bacillus subtilis AH18의 토양내 Genetic Monitoring

Genetic Monitoring of Plant Growth Promoting Rhizobacterium (PGPR), Bacillus subtilis AH18 using Multiplex PCR in Field Soil

정희영|김상달|임종희|우상민
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2009.03|한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)|제37권 제1호|pp. 1-9

1

조회

0

다운로드

1

인용

0

참고문헌

키워드

biocontrolPGPRGenetic monitoringmultiplex PCR (polymerase chain reaction)Bacillus subtilis AH18
주제분류생물학

초록

The genetic monitoring method was developed for the rapid detection of the PGPR and biocontrol agent, B. subtilis AH18 in redpepper field soil by multiplex PCR using sid, aec and cel gene primers. The monitoring of B. subtilis AH18 in the soil was carried by amplified a 2,3-dihydro-2,3-dihydroxy benzoate dehydrogenase [EC:1. 3. 1. 28]gene (sid - 794 bp : EF408238) which is a key enzyme of siderophore synthesis, an auxin efflux carrier gene (aec - 1,052 bp : EF408239) and a cellulase gene (cel - 1,582 bp : EF070194). The natural unsterilized soil was inoculated with B. subtilis AH18 to determine the sensitivity (1.8×105 cfu/g) of multiplex PCR for the rapid dectection and then the strain was monitored successfully in rhizosphere or non-rhizosphere soil of red-pepper cultural soil. At 3 weeks after the treatment, density of the strain was monitored more abundantly in rhizosphere soil.

Abstract

The genetic monitoring method was developed for the rapid detection of the PGPR and biocontrol agent, B. subtilis AH18 in redpepper field soil by multiplex PCR using sid, aec and cel gene primers. The monitoring of B. subtilis AH18 in the soil was carried by amplified a 2,3-dihydro-2,3-dihydroxy benzoate dehydrogenase [EC:1. 3. 1. 28]gene (sid - 794 bp : EF408238) which is a key enzyme of siderophore synthesis, an auxin efflux carrier gene (aec - 1,052 bp : EF408239) and a cellulase gene (cel - 1,582 bp : EF070194). The natural unsterilized soil was inoculated with B. subtilis AH18 to determine the sensitivity (1.8×105 cfu/g) of multiplex PCR for the rapid dectection and then the strain was monitored successfully in rhizosphere or non-rhizosphere soil of red-pepper cultural soil. At 3 weeks after the treatment, density of the strain was monitored more abundantly in rhizosphere soil.

참고문헌 (38)

  1. Boldt, T. S, "Combined use of different gfp reporters for monitoring single-cell activities of a genetically modified PCB degrader in the rhizosphere of alfalfa", FEMS Microbiol. Ecol, 48, pp.139~148 (2004)
  2. Bourque, S. N, "Multiplex polymerase chain reaction for detection and differentiation of the microbial insecticide Bacillus thuringiensis", Appl. Environ. Microb, 59, pp.523~527 (1993)
  3. Cheun, H. I, "Erdenebaatar, K. Kawamoto, and I. Uchida. 2003. Rapid and effective detection of anthrax spores in soil by PCR", J. Appl. Microbiol, 95, pp.728~733 (2003)
  4. Demoz, B. T, "Bacillus subtilis attachment, colonization, and survival on avocado flowers and its mode of action on stem-end rot pathogens", Biol. Control, 37, pp.68~74 (2006)
  5. Gera, R, "Monitoring rhizosphesre colonization by Azospirillum using constitutively expressed gfp gene", Ind. J. Microbiol, 43, pp.219~222 (2003)
  6. Glick, B. R, "Biochemical and genetic mechanisms used by plant growth promoting bacteria" (1999), Imperial College Press
  7. Hui, C. K, "Comparison of Real-Time PCR Assays for Monitoring Serum Hepatitis B Virus DNA Levels during Antiviral Therapy", J Clin. Microbiol, 44, pp.2983~2987 (2006)
  8. 정희경, "진균성 식물병해 방제를 위한 항생물질 생산 길항미생물의 복합제제화", Journal of the Korean Society for Applied Biological Chemistry, 48(1), pp.40~47 (2005), 한국응용생명화학회
  9. 정희경, "Auxin과 Siderophore 생산성 다기능 생물방제균Bacillus subtilis AH18", 한국미생물·생명공학회지, 34(2), pp.94~100 (2006), 한국미생물·생명공학회
  10. 강상재, "시들음병균에 길항하는 미생물의 분리 및 미생물제제를 첨가한 부산물비료의 이화학적 특성", 원예과학기술지, 23(3), pp.342~350 (2005), 한국원예학회
  11. KIM, MI-KWANG, "Multiplex PCR Using Conserved and Species-Specific 16S rDNA Primersfor Simultaneous Detection of Fusobacterium nucleatum and Actinobacillusactinomycetemcomitans", Journal of Microbiology and Biotechnology, 14(1), pp.110~115 (2004), 한국미생물·생명공학회
  12. Kim, M. Y, "Monitoring of genetically modified soybean and processed foods in korean market using PCR", J. Kor. Soc. Agric. Chem. Biotechn., 46, pp.344~347 (2003)
  13. 엄용빈, "Detection of Pathogenic Yersinia enterocoliticaStrains by a Rapid and Specific Multiplex PCR Assay", 대한의생명과학회지, 10(4), pp.333~339 (2004), 대한의생명과학회
  14. Kunst F, "The complete genome sequence of the gram-positive bacterium Bacillus subtilis", Nature, 390, pp.249~256 (1997)
  15. 권도형, "Auxin과 항진균물질을 생산하는 식물생장촉직근권세균의 분리동정 및 특성", Journal of the Korean Society for Applied Biological Chemistry, 47(1), pp.17~21 (2004), 한국응용생명화학회
  16. Lee, E. T, "Selection and antifungal activity of antagonistic bacterium pseudomonas sp. 2112 against red-pepper rotting Phytophthora capsici", Kor. J. Appl. Microbiol. Biotechn, 28, pp.334~340 (2000)
  17. Lee, I. K, "Antifungal antibiotic against fruit rot disease of red pepper form Streptomyces parvullus", Kor. J. Appl. Microbiol. Biotechnol, 18, pp.142~147 (1990)
  18. 이종문, "유기물 첨가가 오이 모잘특병에 대한 미생물 제제의 생물학적 방제 효과 증진에 미치는 영향", 한국균학회지, 31(1), pp.44~49 (2003), 한국균학회
  19. Lee, J. M, "Isolation of siderophore-producing Pseudomonas fluorescens GL7 and its biocontrol activity against root-rot disease", Kor. J. Appl. Microbiol. Biotechn, 27, pp.427~432 (1999)
  20. 이택수, "Multiplex-PCR에 의한 먹는샘물 및 야채류로부터의 병원성 Yersinia enterocolitica의 신속검출", 한국식품영양과학회지, 32(1), pp.35~41 (2003), 한국식품영양과학회
  21. Leonid, N. T, "Production of auxins and auxin - like compounds by ginseng growth - promoting Bacterium Pseudomonas fluorescens KGPP 207", Agric. Chem. Biotechnol, 43, pp.264~268 (2000)
  22. Lindstrom, M, "Detection of Clostridium botulinum by multiplex PCR in foods and feces", Method. Biotechnol, 21, pp.37~45 (2006)
  23. Liu, L, "Induction of systemic resistance in cucumber by plant growth-promoting rhizobacteria: duration of protection and effect of host resistance on protection and root colonization", Phytopathology, 85, pp.1064~1068 (1995)
  24. Mahadi, N. M, "Application of multiplex PCR for rapid determination of cry1 gene profiles of new Bacillus thuringiensis isolates", J. Microbiol. Biotechnol, 8, pp.517~522 (1998)
  25. Martinez, I, "Detection of cdtA, cdtB, and cdtC genes in Campylobacter jejuni by multiplex PCR", Int. J. Med. Microbiol, 296, pp.45~48 (2005)
  26. Oh, Y. J, "Cultural conditions for the improvement in gibberellic acid productivity by a mutant of Gibberella fujikuroi ATCC 12616 - Gibberella fujikuroi G-36", Kor. J. Appl. Microbiol. Biotechnol, 28, pp.152~155 (2000)
  27. Panicker, G, "Detection of pathogenic Vibrio spp. in shellfish by using multiplex PCR and DNA microarrays", Appl. Environ. Microbiol, 70, pp.7436~7444 (2004)
  28. Ping, L, "Signals from the underground: bacrerial volatiles promote growth in Arabidopsis", Trends Pla. Sic., 9, pp.263~266 (2004)
  29. Reva, O. N, "Taxonomic characterization and plant colonizing abilities of some bacteria related to Bacillus amyloliquefaciens and Bacillus subtilis", FEMS Microbiol. Ecol, 48, pp.249~259 (2004)
  30. Rey M. W, "Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species", Genome Biol, 5, pp.R77~ (2004)
  31. Rhoads, J. A, "Characteristics of a new automated real time PCR assay for monitoring HBV patients", J. Clin. Virol, 36, pp.S6~ (2006)
  32. Sergeev, N, "Microarry analysis of Bacillus cereus group virulence factors", J. Microbiol. Meth, 65, pp.488~502 (2006)
  33. Sturtevant, D. B, "Cytokinin production by Rhizobium japonicum", Plant physiology, 39, pp.1247~1452 (1989)
  34. van Loon, L. C, "Systemic resistance induced by rhizosphere bacteria", Annu. Rev. Phytopathol, 36, pp.453~483 (1998)
  35. Wang, S. H, "Identification and characterization of Bacillus anthracis by multiplex PCR on DNA chip", Biosens. Bioelectron, 20, pp.807~813 (2004)
  36. 우상민, "고추역병을 방제하는 PGPR균주 Bacillus subtilis AH18의항진균성 Cellulase 유전자의 Cloning 및 효소특성 조사", 한국미생물·생명공학회지, 34(4), pp.311~317 (2006), 한국미생물·생명공학회
  37. 우상민, "다기능 PGPR균주 Bacillus licheniformis K11이 생산하는 항진균성 Siderophore의 정제와 특성", 한국미생물·생명공학회지, 35(3), pp.128~134 (2007), 한국미생물·생명공학회
  38. 김상달, "생물방제능과 식물성장촉진능을 동시에 가지는 Bacillus licheniformis K11의 non-siderophore 항진균 물질 및 cellulase의 생산조건 확인", 생명과학회지, 17(7), pp.983~989 (2007), 한국생명과학회

관련 논문AI추천

고추역병을 방제하는 PGPR균주 Bacillus subtilis AH18의항진균성 Cellulase 유전자의 Cloning 및 효소특성 조사김상달, 우상민, 정희경한국미생물·생명공학회 · 한국미생물·생명공학회지2006 · 제34권 제4호Bacillus amyloliquefaciens GR4-5 균주의 토양 내 정량 분석김다연, 김병용, 김성일, 김완규, 송재경, 안재형, 원항연한국유기농업학회 · 한국유기농업학회지2015 · 제23권 제4호Auxin과 Siderophore 생산성 다기능 생물방제균Bacillus subtilis AH18김상달, 김진락, 우상민, 정희경한국미생물·생명공학회 · 한국미생물·생명공학회지2006 · 제34권 제2호Bacillus subtilis AH18의 고추역병 방제능과 SiderophoreAH18의 구조분석김상달, 우상민한국미생물·생명공학회 · 한국미생물·생명공학회지2008 · 제36권 제4호Auxin과 ACC Deaminase를 생산하는 사구식물 복원용 근권세균의 선발김상달, 김종국, 임종희한국미생물·생명공학회 · 한국미생물·생명공학회지2008 · 제36권 제4호유용한 바실러스의 토양 접종에 따른 토착 세균 군집의 변화김상윤, 김이슬, 상미경, 송재경, 안주희, 원항연한국미생물·생명공학회 · 한국미생물·생명공학회지2018 · 제46권 제3호

이 간행물의 다른 논문 (12건)

Soraphen 생합성 유전자군을 갖는 점액세균의 분리이차율, 조경연, 현혜숙pp. 10-16Thermus thermophilus HJ6 유래 내열성 DNA Polymerase의 유전자 클로닝 및 발현곽평화, 서민호, 전숭종, 남수완, 김부경, 김한우, 김연희pp. 17-23Characterization of Antimicrobial Substance Produced by Lactobacillus paraplantarum KNUC25 Isolated from Kimchi박유미, 이수진, 김사열, 설경조, 김마리pp. 24-32낙엽진흙버섯 자실체 유래 열수 추출물과 에탄올 침전물의 항바이러스활성이윤희, 남재환, 김성민, 이새미, 김현걸, 신현동, 권두한, 나예슬, 박용일, 김우중pp. 33-41수종의 한약재 추출물의 항산화 활성 및 항진균 활성이용섭, 임융호, 김재영pp. 42-48
원문 보기