국내 고유수종의 고부가가치 자원화를 위한 보길도 자생 황칠나무 수액의 미백효능 연구
For the Utilization of Native Plant Resources as High-value Materials; Evaluation on Demelanizing Activity of Dendropanax Morbifera in Bogildo
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Recently, the integration of products from native plants and high-value biomaterials has been a subject of intense discussion due to its aesthetic and medicinal benefits as well as ecological importance. Although the possible applications and value of native plant resources found in islands are virtually limitless, they had been underestimated due to poor accessibility. In this study, we evaluated the potential use of the tree sap from Dendropanax morbiferathat, populated in Bogildo. It had a high-value as a demelanizing agent. Melanogenesis is a biosynthetic process which is needed in order to produce pigment melanin. In this study, we analyzed the effect of partially purified extracts from the tree sap of D. morbiferaon melanogenesis. We first examined the activity of the whole sap on biosynthesis of melanin, and observed that the treatment of the sap decreased the melanin synthesis up to 54.9%. Subsequently, we investigated the inhibitory effect of fractions, partially extracted with several organic solvents. The functional activity related to the down-regulation of melanin biosynthesis was found in the fraction extracted with ethyl acetate. The most often reported mechanism of the inhibition of melanogenesis is the down-regulation of tyrosinase, and thus we examined whether the ethyl acetate fraction modulates the expression of tyrosinase. We found that at the concentration of 5ug/mL, the ethyl acetate fraction transcriptionally inhibits the tyrosinase gene expression without any sign of apoptosis. Taken together, these results provide some insights on the molecular basis of the skin-lightening activity of the sap of D. morbifera as well as an effective search for the active ingredients.
Abstract
Recently, the integration of products from native plants and high-value biomaterials has been a subject of intense discussion due to its aesthetic and medicinal benefits as well as ecological importance. Although the possible applications and value of native plant resources found in islands are virtually limitless, they had been underestimated due to poor accessibility. In this study, we evaluated the potential use of the tree sap from Dendropanax morbiferathat, populated in Bogildo. It had a high-value as a demelanizing agent. Melanogenesis is a biosynthetic process which is needed in order to produce pigment melanin. In this study, we analyzed the effect of partially purified extracts from the tree sap of D. morbiferaon melanogenesis. We first examined the activity of the whole sap on biosynthesis of melanin, and observed that the treatment of the sap decreased the melanin synthesis up to 54.9%. Subsequently, we investigated the inhibitory effect of fractions, partially extracted with several organic solvents. The functional activity related to the down-regulation of melanin biosynthesis was found in the fraction extracted with ethyl acetate. The most often reported mechanism of the inhibition of melanogenesis is the down-regulation of tyrosinase, and thus we examined whether the ethyl acetate fraction modulates the expression of tyrosinase. We found that at the concentration of 5ug/mL, the ethyl acetate fraction transcriptionally inhibits the tyrosinase gene expression without any sign of apoptosis. Taken together, these results provide some insights on the molecular basis of the skin-lightening activity of the sap of D. morbifera as well as an effective search for the active ingredients.
참고문헌 (29)
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