배양액의 유기영양물이 Chitinase 생산에 미치는 영향
Effects of Organic Nutrients on ChitinaseProduction in Minimal Media.
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Abstract
Four chitinase producing bacteria, Arthrobacter nicotinae CH4, Arthrobacter nicotinae CH13, Arthrobacter sp. CH5 and Micrococcus sp. CH3, were isolated from small crabs and shrimps. We investigated the optimum medium condition for the production of enzyme and high cell mass. The preferable medium composition was as follows: colloidal chitin 0.1%(w/v), glycerol 0.25%(w/v) and yeast extract 0.05%(w/v) in minimal midium (K2HPO4 0.7 g/l, KH2PO4 0.3 g/l, MgSO4.5H2O 0.5 g/l, FeSO4.7H2O 0.01 g/l, ZnSO4 0.001 g/l, MnCl2 0.001 g/l, pH 7.0). This cell culture medium could be used directly as sample for measuring chitinase activity. Because it hardly contained reducing sugar such as glucose (blank value ~- 0), the detected reducing sugar can be considered as a chitinase reaction product. The results can be used for easy preparation method for determination of enzyme activity and analysis of enzyme-substrate reaction in step of screening of chitinase producing bacteria.
참고문헌 (22)
- "Enzymatic hydrolysis of pretreated chitin by Aspergillus carmeus chitinase"
- "Purification and characterization of thermostable chitinase from Bacillus lichenifomis KFB-C14", 24:, pp.567~573 (1996)
- "Isolation of microorganism producing thermostable chitinase and some cultural characteristics for chitinase production", 24:, pp.560~566 (1996)
- "Chitinase system of a strain of Streptomyces griseus", pp.522~534 (1958)
- "Microbial production of N-acetylglucosamine by Arthrobacter nicotianae", 35:, pp.1188~1192 (2003)
- "Membrane-ative drugs potentiate the killing of tumor cells by D-glucosamine", pp.1172~1176 (1980)
- "Isolation of microorganism producing thermostable chitinase and some cultural characteristics for chitinase production", 24:, pp.560~566 (1996)
- "Powdered chitin agar as a selective medium for enumeration of Actinomyces in water soil", pp.422~429 (1975)
- "http:", ngg.html
- "Isolation of microorganism producing of chitinase and its enzymatic characteristics", 23
- "Isolation of Aeromonas hydrophila and chitinolytic properties", 25, pp.151~158 (1997)
- "Use of chitinous polymers in food", pp.85~97 (1984)
- "Purification and characterization of chitinase from antagonistic bacteria Pseudomonas sp", 26:, pp.515~522 (1998)
- "The production and purification of chitinase from Aeromonas salmonicida YA7-625", pp.515~522 (1990)
- "The production and purification of chitinase from Aeromonase salmonicida YA7-625", pp.599~606 (1990)
- "Identification and cultural characterization of Streptomyces lydicus G-23 for producing chitinase"
- "A photometric adsorption of somogyi method for determination of glucose", pp.376~380 (1944)
- "Chitinolytic enzymes: an exploration", 26(lytic enzymes), pp.473~483 (2000)
- "Extracellular chitinase from a Streptomyces sp", pp.150~159 (1954)
- "The hydrolysis od chitin by concentrated hydrochloric acid and the preparation of low molecluar weight substrates for lysozyme", pp.245~255 (1964)
- "Immobilization of chitinolytic enzymes and continuous production of N-acetylglucosamine with the immobilized enzyme", (mjo), pp.168~172 (1991)
- "A new regent for determination of sugar"