Trichoderma harzinum SJG-99721의 체외 분비 Chitinase 생산에 미치는 생물반응기에서의 반응 최적화 연구
Optimization of Environmental Parameters for Extracellular Chitinase Production by Trichoderma harzinum SJG-99721 in Bioreactor
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Abstract
A self-directing optimization procedure was applied to determine the best environmental factors in operating the bioreactor. The self-directing optimization process was employed to determine the best conditional combination of multiparameters, pH, temperature, aeration rate and mixing rate for maximal production of chitinase by Trichoderma harzianum SJG-99721 in batch mode fermentation. Among these factors, the parameters of pH and aeration rate were found to be particularly important on mycellial growth and chitinase activity. pH 4.89, an aeration rate of 3.22 l per minute and an agitation rate of 225 rpm was found to be the best combination. By the optimization, chitinase activity was dramatically increased from an initial value of 4.221 U under basic conditions to a final value of 16.825 U.
참고문헌 (10)
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