청국장으로부터 면역증강활성이 우수한Bacillus pumilus JB-1의 뷴리 및 분리균의청국장 발효특성
Isolation of Immuno-stimulating Strain Bacillus pumilusJB-1 from Chungkook-jang and FermentationalCharacteristics of JB-1.
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초록
고품질, 기능성 청국장 제조를 위한 균주 개발 연구의 일환으로, 재래식 청국장으로부터 생육이 빠르며, 면역증강활성이 우수한 JB-1 균주를 분리하였다. 동정결과, JB-1 균주는 Bacillus pumilus로 확인되었으며 Bacillus pumilus KCTC 10461BP로 균주기탁 되었다. JB-1으로 발효시킨 청국장은 관능성과 영양성이 우수하며 물추출물 2 mg/ml 의 농도에서 410% 정도의 면역세포 증식율을 나타내었으며, 산업적 규모의 6 kg의 대두증자에 20 ml 배양액을 접종함으로서 16 시간 이내에 저이취와 높은 생균수 및 아미노산 함량의 청국장 제조가 가능함을 확인하였다.
Abstract
To produce functional and high nutritional chungkook-jang, an immuno-stimulating and rapid growing bacterium was isolated from one of Korean traditional food, chungkook-jang. The isolated bacterium was identified as Bacillus pumilus and deposited in Korean Collection for Type Culture (KCTC 10461BP). The chungkook-jang fermented by JB-1 has good taste and pleasant smell with high content of free amino acids, and the water extract of chungkook-jang showed 410% of immuno-stimulation activity at concentration of 2 mg/ml. The rapid fermentation in 16 h is achieved by inoculation of 20 ml culture broth of JB-1 into 6 kg cooked soybean in commercial scale at 40oC.
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