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역병에 대한 Enterobacter asburiae ObRS-5 처리의 유도저항성 발현

Induction of systemic resistance against Phytophthora blight by Enterobacter asburiae ObRS-5 with enhancing defense-related genes expression

박진우|박인철|전용희|안시현|안재형|윤영건|김다연
한국환경생물학회 (Korean Society Of Environmental Biology)|2020.12|환경생물 (Korean Journal of Environmental Biology)|제38권 제4호|pp. 724-732|DOI 10.11626/KJEB.2020.38.4.724

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키워드

Phytophthora capsicibiological controlEnterobacter asburiae ObRS-5induced systemic resistancered pepper plants

초록

본 연구에서는 기 선발한 Enterobacter asburiae ObRS-5 균주를 1×108 cfu mL-1 농도로 고추에 관주 처리했을 때 Phytophthora capsici에 의한 고추역병을 74.6% 방제하는 효과가 있었다. E. asburiae ObRS-5 균주에 의한 고추역병 방제 메커니즘을 확인하기 위해 고추의 PR1, PR4 및 PR10 유전자를 특이적으로 증폭하는 프라이머를 이용하여 quantitative PCR을 수행하였다. 그 결과 E. asburiae ObRS-5 균주를 처리한 고추에서 대조구와 비교하여 상기 세 가지 유전자의 발현이 모두 높은 수준을 유지하였다. 또한 E. asburiae ObRS-5 균주는 고추의 생육을 억제하지 않으면서 ISR 반응을 유도하는 것으로 나타났다. 이러한 결과를 통하여 P. capsici이 침입할 때 E. asburiae ObRS-5 균주가 매개하는 ISR 메커니즘을 통해 Phytophthora 역병의 제어가 가능한 것으로 사료된다.

Abstract

Phytophthora capsici is the organism that causes Phytophthora blight which infects red pepper plants prolifically, ultimately leading to crop loss. A previous study revealed that Enterobacter asburiae ObRS-5 suppresses Phytophthora blight in both red pepper and Ligularia fischeri plants. In order to determine whether the induced systemic resistance (ISR) was triggered by pre-infection with the ObRS-5 strain, we conducted quantitative PCR using primers for PR1, PR4, and PR10, which correlate with systemic resistance in red-pepper plants. In our results, red pepper plants treated with the ObRS-5 strain demonstrated increased expression of all three systemic resistance genes when compared to controls in the glasshouse seedling assay. In addition, treatment of red peppers with the ObRS-5 strain led to reduced Phytophthora blight symptoms caused by P. capsici, whereas all control seedlings were severely affected. Perhaps most importantly, E. asburiae ObRS-5 was shown to induce the ISR response in red peppers without inhibiting growth. These results support that the defense mechanisms are triggered by ObRS-5 strain prior to infection by P. capsici and ObRS-5 strain-mediated ISR action are linked events for protection to Phytophthora blight.

참고문헌 (45)

  1. Bae SJ, "Trichoderma metabolites as biological control agents against Phytophthora pathogens", Biol. Control, 92, pp.128~138 (2016)
  2. Baum C, "Increasing the productivity and product quality of vegetable crops using arbuscular mycorrhizal fungi : a review", Sci. Hortic, 187, pp.131~141 (2015)
  3. Berner I, "Characterization of ferrioxamine E as the principal siderophore of Erwinia herbicola(Enterobacter agglomerans)", Biol. Met, 1, pp.51~56 (1988)
  4. Bünemann EK, "Soil quality - a critical review", Soil Biol. Biochem., 120, pp.105~125 (2018)
  5. Chernin L, "Chitinolytic Enterobacter agglomerans antagonistic to fungal plant pathogens", Appl. Environ. Microbiol, 61, pp.1720~1726 (1995)
  6. Cooley MB, "Colonization of Arabidopsis thaliana with Salmonella enterica and enterohemorrhagic Escherichia coli O157 : H7 and competition by Enterobacter asburiae", Appl. Environ. Microbiol, 69, pp.4915~4926 (2003)
  7. Elliott M, "Evaluation of several commercial biocontrol products on European and North American populations of Phytophthora ramorum", Biocontrol Sci. Technol, 19, pp.1007~1021 (2009)
  8. Haggag W, "Colonization of peanut roots by biofilm-forming Paenibacillus polymyxa initiates biocontrol against crown rot disease", J. Appl. Microbiol, 104, pp.961~969 (2008)
  9. Hardham AR, "Pathogen profile Phytophthora cinnamomi", Mol. Plant Pathol, 19, pp.260~285 (2005)
  10. Heil M, "Induced systemic resistance(ISR)against pathogens in the context of induced plant defences", Ann. Bot, 89, pp.503~512 (2002)
  11. Hong CE, "Biocontrol activity of Paenibacillus polymyxa AC-1 against Pseudomonas syringae and its interaction with Arabidopsis thaliana", Microbiol. Res, 185, pp.13~21 (2016)
  12. Jee HJ, "Phytophthora diseases in Korea", pp.87~114 (2000), Rural Development Administration, National Institute of Agricultural Science and Technology, Plant Pathology Division
  13. Jetiyanon K, "Lipopolysaccharide of Enterobacter asburiae strain RS83: A bacterial determinant for induction of early defensive enzymes in Lactuca sativa against soft rot disease", Biol. Control, 67, pp.301~307 (2013)
  14. Joo GJ, "Production of an anti-fungal substance for biological control of Phytophthora capsici causing Phytophthora blight in red-peppers by Streptomyces halstedii", Biotechnol. Let., 27, pp.201~205 (2005)
  15. 강대선, "Defense Response and Suppression of Phytophthora Blight Disease of Pepper by Water Extract from Spent Mushroom Substrate of Lentinula edodes", The Plant Pathology Journal, 33(3), pp.264~275 (2017), 한국식물병리학회
  16. 강효중, "고추 역병 피해 해석과 방제가 필요한 발병수준의 설정", 식물병 연구, 17(1), pp.1~12 (2011), 한국식물병리학회
  17. Kim BS, "Phytophthora blight of pepper and genetic control of the disease", Cur. Res. Agric. Life Sci., 32, pp.111~117 (2014)
  18. Dayeon Kim, "Biological Control of Gom-chwi (Ligularia fischeri) Phytophthora Root Rot with Enterobacter asburiae ObRS-5 to Suppress Zoosporangia Formation and Zoospores Germination", The Plant Pathology Journal, 36(3), pp.244~254 (2020), 한국식물병리학회[DOI]
  19. 이호용, "Arbuscular mycorrhizal fungus인 Glomus manihot 균 접종이 고추의 성장과 영양분 흡수에 미치는 영향", 환경생물, 21(3), pp.11~296 (2003), 한국환경생물학회
  20. 김정순, "Evaluation of Resistance in Hot Pepper Germplasm to Phytophthora Blight on Biological Assay", 원예과학기술지, 28(5), pp.802~809 (2010), 한국원예학회
  21. 김성택, "점액세균 Myxococcus sp. KYC 1126을 이용한 고추 역병 생물학적 방제 효능", 식물병 연구, 17(2), pp.121~128 (2011), 한국식물병리학회
  22. Kloepper JW, "Induced systemic resistance and promotion of plant growth by Bacillus spp", Phytopathology, 94, pp.1259~1266 (2004)
  23. Lee SH, "An antibiotic fusaricidin : a cyclic depsipeptide from Paenibacillus polymyxa E681 induces systemic resistance against Phytophthora blight of red-pepper", Phytoparasitica, 41, pp.49~58 (2013)
  24. 이상준, "국내 고추 역병균의 병원성 분화", 식물병 연구, 16(2), pp.153~157 (2010), 한국식물병리학회
  25. 임종희, "다기능 PGPR균주들의 기작별 상호보완형 컨소시엄 구성을 통한 고추역병 방제 및 고추생장촉진", Journal of Applied Biological Chemistry, 52(3), pp.116~120 (2009), 한국응용생명화학회
  26. Livak KJ, "Analysis of relative gene expression data using realtime quantitative PCR and the 2∆∆C(T) Method", Methods, 25, pp.402~408 (2001)
  27. Lopez-Gutierrez JC, "Quantification of a novel group of nitrate-reducing bacteria in the environment by real-time PCR", J. Microbiol. Methods, 57, pp.399~407 (2004)
  28. Macas-Rodrguez L, "Trichoderma atroviride promotes tomato development and alters the root exudation of carbohydrates, which stimulates fungal growth and the biocontrol of the phytopathogen Phytophthora cinnamomi in a tripartite interaction system", FEMS Microbiol. Ecol., 94, pp.fiy137~ (2018)
  29. Mahaffee WF, "Effects of seed factors on spermosphere and rhizosphere colonization of cotton by Bacillus subtilis GB03", Phytopathology, 83, pp.1120~1125 (1993)
  30. Mojdeh Maleki, "Screening of Rhizobacteria for Biological Control of Cucumber Root and Crown Rot Caused by Phytophthora drechsleri", The Plant Pathology Journal, 27(1), pp.78~84 (2011), 한국식물병리학회
  31. Mansoori B, "Evaluating cucurbit seedling resistance to Phytophthora drechsleri", Plant Dis., 66, pp.373~ (1982)
  32. 박진우, "Evaluation of Bacillus subtilis Native Strains for Plant Growth Promotion and Induced Systemic Resistance in Tomato and Red-pepper", 농약과학회지, 14(4), pp.407~414 (2010), 한국농약과학회
  33. Park K, "Recent Trends in PGPR Research for Sustainable Crop Productivity", pp.200~205 (2016), Scientific Publishers
  34. 박세정, "고추 역병과 탄저병에 대한 친환경유기농자재의 방제 효과", 식물병 연구, 18(1), pp.1~9 (2012), 한국식물병리학회[DOI]
  35. Parra G, "Resistance to mefenoxam and metalaxyl among field isolates of Phytophthora capsici causing Phytophthora blight of bell pepper", Plant Dis., 85, pp.1069~1075 (2001)
  36. Porter SS, "Beneficial microbes ameliorate abiotic and biotic sources of stress on plants", Funct. Ecol., 34, pp.2075~2086 (2019)
  37. Akbar Shirzad, "Antagonistic Potential of Fluorescent Pseudomonads and Control of Crown and Root Rot of Cucumber Caused by Phythophtora drechsleri", The Plant Pathology Journal, 28(1), pp.1~9 (2012), 한국식물병리학회
  38. 소재우, "고추 역병균의 접종원에 따른 역병 저항성의 유전 양식", 식물병 연구, 18(4), pp.317~323 (2012), 한국식물병리학회[DOI]
  39. Szczech M, "The effect of mode of application of Bacillus subtilis RB14C on its efficacy as a biocontrol agent against Rhizoctonia solani", J. Phytopathol, 154, pp.370~377 (2006)
  40. Utkhede RS, "Biology and control of apple crown rot caused by Phytophthora cactorum: A review", Phytoprotection, 67, pp.1~13 (1986)
  41. van Loon LC, "Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var. ‘Samsum’ and ‘Samsun NN’ II. Changes in protein constitution after infection with tobacco mosaic virus", Virol, 40, pp.199~211 (1970)
  42. van Loon LC, "The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins", Physiol. Mol. Plant Pathol., 55, pp.85~97 (1999)
  43. 우상민, "고추역병을 방제하는 PGPR균주 Bacillus subtilis AH18의항진균성 Cellulase 유전자의 Cloning 및 효소특성 조사", 한국미생물·생명공학회지, 34(4), pp.311~317 (2006), 한국미생물·생명공학회
  44. Yang R, "The inhibitory mechanisms by mixtures of two endophytic bacterial strains isolated from Ginkgo biloba against pepper Phytophthora blight", Biol. Control, 85, pp.59~67 (2015)
  45. Zhang YL, "Genetic determinants of the defense response of resistant and susceptible pepper (Capsicum annuum) cultivars infected with Phytophthora capsici (Oomycetes; Pythiaceae)", Genet. Mol. Res., 12, pp.3605~3621 (2013)

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