Antimicrobial Effect of Inula britannica Flower Extract against Methicillin-resistant Staphylococcus aureus
0
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
The antimicrobial effect of the methanol extract of Inula britannica flowers against methicillin resistant Staphylococcus aureus (MRSA) was investigated. It was confirmed that the methanol extract is mainly composed of quercetin, which has antimicrobial properties. The antimicrobial effect of the methanol extract against 3 MRSA strains was determined by the disc diffusion method. The minimal inhibitory concentrations were ranged from 0.625 mg/ml to 1.25 mg/ml, and the minimum bactericidal concentrations were 2.5 mg/ml. Time kill kinetics revealed bactericidal activities, and the morphological alterations in S. aureus ATCC 33591 treated with the extract were observed using a scanning electron microscope. The methanol extract affected the expression of the resistant genes, mecA, mecI, and mecRI in mRNA. Therefore, the methanol extract of I. britannica flowers clearly demonstrated an antimicrobial effect against MRSA and these results suggest a potential for application as a natural antimicrobial agent.
Abstract
The antimicrobial effect of the methanol extract of Inula britannica flowers against methicillin resistant Staphylococcus aureus (MRSA) was investigated. It was confirmed that the methanol extract is mainly composed of quercetin, which has antimicrobial properties. The antimicrobial effect of the methanol extract against 3 MRSA strains was determined by the disc diffusion method. The minimal inhibitory concentrations were ranged from 0.625 mg/ml to 1.25 mg/ml, and the minimum bactericidal concentrations were 2.5 mg/ml. Time kill kinetics revealed bactericidal activities, and the morphological alterations in S. aureus ATCC 33591 treated with the extract were observed using a scanning electron microscope. The methanol extract affected the expression of the resistant genes, mecA, mecI, and mecRI in mRNA. Therefore, the methanol extract of I. britannica flowers clearly demonstrated an antimicrobial effect against MRSA and these results suggest a potential for application as a natural antimicrobial agent.
참고문헌 (24)
- Hirai I, "Characterization of anti-Staphylococcus aureus activity of quercetin", Int. J. Food Sci. Tech, 45, pp.1250~1254 (2010)
- Högberg LD, "The global need for effective antibiotics: challenges and recent advances", Trends Pharmacol. Sci, 31, pp.509~515 (2010)
- 황보미, "해양 생물 유래의 항균 펩타이드 및 작용 기작", 한국미생물·생명공학회지, 38(1), pp.19~23 (2010), 한국미생물·생명공학회
- Khan AL, "Secondary metabolites from Inula britannica L. and their biological activities", Mol, 15, pp.1562~1577 (2010)
- Kubo I, "Antibacterial activity of polygodial", Phytother. Res, 19, pp.1013~1017 (2005)
- Kuwahara-Arai K, "Suppression of methicillin resistance in a mecA-containing pre-methicillin-resistant Staphylococcus aureus strain is caused by the mecI-mediated repression of PBP 2’ production", Antimicrob. Agents Chemother, 40, pp.2680~2685 (1996)
- 이장현, "Screening of Antiviral Medicinal Plants against Avian Influenza Virus H1N1 for Food Safety", 한국축산식품학회지, 30(2), pp.345~350 (2010), 한국축산식품학회
- Lee JH, "Antimicrobial properties of whole milk with Inula britannica extract against Bacillus cereus strains during storage", Michwissenschaft, 67, pp.315~317 (2012)
- Ji-Won Lee, "Effect of Saliva miltiorrhiza Bunge on Antimicrobial Activity and Resistant Gene Regulation against Methicillin-Resistant Staphylococcus aureus (MRSA)", The Journal of Microbiology, 45(4), pp.350~357 (2007), 한국미생물학회
- Lee JW, "Antimicrobial effect and resistant regulation of Glycyrrhiza uralensis on methicillin-resistant Staphylococcus aureus", Nat. Prod. Res, 23, pp.101~111 (2009)
- Li X, "The role of femA regulating gene on methicillin-resistant Staphylococcus aureus clinical isolates", Med. Mal. Infect, 42, pp.218~225 (2012)
- Lim TT, "Genetic organization of mecA and mecA-regulatory genes in epidemic methicillinresistant Staphylococcus aureus from Australia and England", J. Antimicrob. Chemoth, 50, pp.819~824 (2002)
- Maoz M, "Antimicrobial effects of aqueous plant extracts on the fungi Mircosporum canis and Trichophyton rubrum and on three bacterial species", Lett. Appl. Microbiol, 26, pp.61~63 (1998)
- Nyman JA, "Cost of screening intensive care unit patients for methicillin-resistant Staphylococcus aureus in hospitals", Am. J. Intect. Control, 39, pp.27~34 (2011)
- O’Shea S, "In vitro activity of Inula helenum against clinical Staphylococcus aureus strains including MRSA", Br. J. Biomed. Sci, 66, pp.186~189 (2009)
- Shan B, "The in vitro antibacterial activity of dietary spice and medicinal herb extracts", Int. J. Food Microbiol, 117, pp.112~119 (2007)
- Song QH, "Hepatoprotective effects of Inula britannica on hepatic injury in mice", Phytother. Res, 14, pp.180~186 (2000)
- Soon JM, "Foodborne disease in Malaysia: A review", Food Control, 22, pp.823~830 (2011)
- Talib WH, "Antimicrobial, cytotoxicity and phytochemical screening of Jordanian plants used in traditional medicine", Mol, 15, pp.1811~1824 (2010)
- Tanaka H, "A new biisoflavonoid from the roots of Erythrina variegata", Nat. Prod. Commun, 5, pp.1781~1784 (2010)
- Todd ECD, "Microbiological safety standards and public health goals to reduce food-borne disease", Meat Sci, 66, pp.33~43 (2003)
- Woisky R, "Analysis of propolis: some parameters and procedures for chemical quality control", J. Apic. Res, 37, pp.99~105 (1998)
- Xu HX, "The antibacterial principle of Caesalpina sappan", Phytother. Res, 18, pp.647~651 (2004)
- Yang JH, "Antioxidant properties of several commercial mushrooms", Food Chem, 77, pp.229~235 (2002)