Antimicrobial Activity of Chamaecyparis obtusa (Siebold & Zucc.) Endl. Xylem Extract against Methicillin-Resistant Staphylococcus aureus
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In this study, the antibacterial activity of Chamaecyparis obtusa (Siebold & Zucc.) Endl. Xylem (COX) was investigated. The antimicrobial activity of the EEC was evaluated using minimal inhibitory concentration (MIC) tests, colorimetric methods with the MTT assay, checkerboard dilution tests, and by testing against six standard strains of methicillin-resistant S. aureus and one standard methicillin-susceptible S. aureus (MSSA) strain. Chamaecyparis obtusa (Siebold & Zucc.) Endl. is for 7 strains the minimum inhibitory concentrations (MICs) are in the range of 125-250 ㎍/mL and the FICI values of EEC + AM, EEC + OX and EEC + VC are 0.01-0.03 and 0.04-0.09 and 0.07-0.25 showed an increase in synergy with the checkerboard method performed using MRSA and MSSA strains. When combined together, this effect were dramatically increased. The time-kill curve was measured at OD 600 ㎚ and showed that the number of bacteria was significantly reduced by combining AM, OX and EEC, which dropped below the lowest measurable limit after 48 h. These results demonstrate that Chamaecyparis obtusa (Siebold & Zucc.) Endl. can be a natural antibacterial agent in MRSA.
Abstract
In this study, the antibacterial activity of Chamaecyparis obtusa (Siebold & Zucc.) Endl. Xylem (COX) was investigated. The antimicrobial activity of the EEC was evaluated using minimal inhibitory concentration (MIC) tests, colorimetric methods with the MTT assay, checkerboard dilution tests, and by testing against six standard strains of methicillin-resistant S. aureus and one standard methicillin-susceptible S. aureus (MSSA) strain. Chamaecyparis obtusa (Siebold & Zucc.) Endl. is for 7 strains the minimum inhibitory concentrations (MICs) are in the range of 125-250 ㎍/mL and the FICI values of EEC + AM, EEC + OX and EEC + VC are 0.01-0.03 and 0.04-0.09 and 0.07-0.25 showed an increase in synergy with the checkerboard method performed using MRSA and MSSA strains. When combined together, this effect were dramatically increased. The time-kill curve was measured at OD 600 ㎚ and showed that the number of bacteria was significantly reduced by combining AM, OX and EEC, which dropped below the lowest measurable limit after 48 h. These results demonstrate that Chamaecyparis obtusa (Siebold & Zucc.) Endl. can be a natural antibacterial agent in MRSA.