Synergistic Antibacterial Activity of an Active Compound Derived from Sedum takesimense against Methicillin-Resistant Staphylococcus aureus and Its Clinical Isolates
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
There are a growing number of reports of hospital-acquired infections caused by pathogenic bacteria, especially methicillin-resistant Staphylococcus aureus (MRSA). Many plant products are now being used as a natural means of exploring antimicrobial agents against different types of human pathogenic bacteria. In this research, we sought to isolate and identify an active molecule from Sedum takesimense that has possible antibacterial activity against various clinical isolates of MRSA. NMR analysis revealed that the structure of the HPLC-purified compound was 1,2,4,6-tetra-Ogalloyl- glucose. The minimum inhibitory concentration (MIC) of different extract fractions against numerous pathogenic bacteria was determined, and the actively purified compound has potent antibacterial activity against multidrug-resistant pathogenic bacteria, i.e., MRSA and its clinical isolates. In addition, the combination of the active compound and β-lactam antibiotics (e.g., oxacillin) demonstrated synergistic action against MRSA, with a fractional inhibitory concentration (FIC) index of 0.281. The current research revealed an alternative approach to combating pathogenesis caused by multi-drug resistant bacteria using plant materials. Furthermore, using a combination approach in which the active plant-derived compound is combined with antibiotics has proved to be a successful way of destroying pathogens synergistically.
Abstract
There are a growing number of reports of hospital-acquired infections caused by pathogenic bacteria, especially methicillin-resistant Staphylococcus aureus (MRSA). Many plant products are now being used as a natural means of exploring antimicrobial agents against different types of human pathogenic bacteria. In this research, we sought to isolate and identify an active molecule from Sedum takesimense that has possible antibacterial activity against various clinical isolates of MRSA. NMR analysis revealed that the structure of the HPLC-purified compound was 1,2,4,6-tetra-Ogalloyl- glucose. The minimum inhibitory concentration (MIC) of different extract fractions against numerous pathogenic bacteria was determined, and the actively purified compound has potent antibacterial activity against multidrug-resistant pathogenic bacteria, i.e., MRSA and its clinical isolates. In addition, the combination of the active compound and β-lactam antibiotics (e.g., oxacillin) demonstrated synergistic action against MRSA, with a fractional inhibitory concentration (FIC) index of 0.281. The current research revealed an alternative approach to combating pathogenesis caused by multi-drug resistant bacteria using plant materials. Furthermore, using a combination approach in which the active plant-derived compound is combined with antibiotics has proved to be a successful way of destroying pathogens synergistically.
참고문헌 (35)
- Ventola CL, "The antibiotic resistance crisis : part 1 : causes and threats", Pharm. Ther, 40, pp.277~283 (2015)
- "Antibiotic Resistance Threats in the United States"[URL]
- Tong SY, "Staphylococcus aureus infections : epidemiology, pathophysiology, clinical manifestations, and management", Clin. Microbiol. Rev, 28, pp.603~661 (2015)
- Kaur DC, "Study of antibiotic resistance pattern in methicillin resistant Staphylococcus aureus with special reference to newer antibiotic", J. Glob. Infect. Dis, 7, pp.78~84 (2015)
- French GL, "The continuing crisis in antibiotic resistance", Int. J. Antimicrob. Agents, 36, pp.S3~ (2010)
- isolates at a tertiary care hospital in Mangalore, South India, "J. Lab. Physicians", 2010, 82~84
- Okwu MU, "Methicillin-resistant Staphylococcus aureus(MRSA)and anti-MRSA activities of extracts of some medicinal plants : a brief review", AIMS Microbiol, 5, pp.117~137 (2019)
- Compean KL, "Antimicrobial activity of plant secondary metabolites : a review", Res. J. Med. Plant, 8, pp.204~213 (2014)
- Sato M, "Synergistic effects of mupirocin and an isoflavanone isolated from Erythrina variegata on growth and recovery of methicillin-resistant Staphylococcus aureus", Int. J. Antimicrob. Agents, 24, pp.241~246 (2004)
- Choi JG, "Antibacterial activity of Ecklonia cava against methicillinresistant Staphylococcus aureus and Salmonella spp", Foodborne Pathog. Dis, 7, pp.435~441 (2010)
- Aqil F, "Effect of certain bioactive plant extracts on clinical isolates of β-lactamase producing methicillin resistant Staphylococcus aureus", J. Basic. Microbiol, 45, pp.106~114 (2005)
- Aqil F, "Evaluation of anti-methicillin-resistant Staphylococcus aureus(MRSA)activity and synergy of some bioactive plant extracts", Biotechnol. J, 1, pp.1093~1102 (2006)
- Cha JD, "Synergistic effect between cryptotanshinone and antibiotics against clinic methicillin and vancomycin-resistant Staphylococcus aureus", Evid. Based Complement. Alternat. Med, 2014, pp.450572~ (2014)
- Yoon MY, "Effect of polyacetylenic acids from Prunella vulgaris on various plant pathogens", Lett. Appl. Microbiol, 51, pp.511~517 (2010)
- Yoon MY, "Potent in vivo antifungal activity against powdery mildews of pregnane glycosides from the roots of Cynanchum wilfordii", J. Agric. Food. Chem, 59, pp.12210~12216 (2011)
- Gangadhar M, "Isolation and characterisation of gallic acid from Terminalia bellerica and its effect on carbohydrate regulatory system in vitro", Int. J. Res. Ayurveda. Pharm, 2, pp.559~562 (2011)
- Hisham DMN, "Identification and isolation of methyl gallate as a polar chemical marker for Labisia pumila Benth", J. Trop. Agric. Food. Sci, 39, pp.279~284 (2011)
- Tanaka T, "7-O-Galloyl-(+)-catechin and 3-O-galloylprocyanidin B-3 from Sanguisorba officinalis", Phytochemistry, 22, pp.2575~2578 (1983)
- Tanaka T, "Punicafolin, an ellagitannin from the leaves of Punica grantum", Phytochemistry, 24, pp.2075~2078 (1985)
- Thuong PT, "Anti-oxidant constituents from Sedum takesimense", Phytochemistry, 68, pp.2432~2438 (2007)
- Wang KJ, "Phenolic antioxidants from Chinese toon(fresh young leaves and shoots of Toona sinensis)", Food Chem, 101, pp.365~371 (2007)
- Xin-Min C, "Galloylarbutin and other polyphenols from Bergenia purpurascens", Phytochemistry, 26, pp.515~517 (1987)
- Yang CM, "The in vitro activity of geraniin and 1, 3, 4, 6-tetra-O-galloyl-β-D-flucose isolated from Phyllanthus urinaria against herpes simplex virus type 1 and type 2 infection", J. Ethnopharmacol, 110, pp.555~558 (2007)
- Yuan GQ, "Isolation of methyl gallate from Toxicodendron sylvestre and its effect on tomato bacterial wilt", Plant Dis, 96, pp.1143~1147 (2012)
- Dickson RA, "Antimicrobial, resistance-modifying effects, antioxidant and free radical scavenging activities of Mezoneuron benthamianum Bail., Securinega virosa Roxb. and Wlld. and Microglossa pyrifolia Lam", Phytother. Res, 20, pp.41~45 (2006)
- Wikler MA, "Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically : approved standard", CLSI(NCCLS), 26, pp.M7~A9 (2012)
- Norden CW, "Comparison of techniques for measurement of in vitro antibiotic synergism", J. Infect. Dis, 140, pp.629~633 (1979)
- 유용욱, "Antimicrobial Activity of Berberine Alone and in Combination with Ampicillin or Oxacillin Against Methicillin-Resistant Staphylococcus aureus", Journal of Medicinal Food, 8(4), pp.454~461 (2005), 한국식품영양과학회
- Vu TT, "Effect of gallotannins derived from Sedum takesimense on tomato bacterial wilt", Plant Dis, 97, pp.1593~1598 (2013)
- Odontuya, G, "Anti-oxidative, acetylcholinesterase and pancreatic lipase inhibitory activities of compounds from Dasiphora fruticosa Myricaria alopecuroides and Sedum hybridum", Mong. J. Chem, 17, pp.42~49 (2016)
- Bensouici C, "Compounds from Sedum caeruleum with antioxidant, anticholinesterase, and antibacterial activities", Pharm. Biol, 54, pp.174~179 (2016)
- 이대성, "Synergistic Effect between Dieckol from Ecklonia stolonifera and β-Lactams against Methicillin-resistant Staphylococcus aureus", Biotechnology and Bioprocess Engineering, 13(6), pp.758~764 (2008), 한국생물공학회
- Lee DS, "Antibacterial and synergic effects of gallic acid-grafted-chitosan with β-lactams against methicillin-resistant Staphylococcus aureus(MRSA)", Can. J. Microbiol, 60, pp.629~638 (2014)
- Cantoni LAMJ, "Comparative efficacy of amoxicillin-clavulanate, cloxacillin, and vancomycin against methicillin-sensitive and methicillin-resistant Staphylococcus aureus endocarditis in rats", J. Infect. Dis, 159, pp.989~993 (1989)
- Côté H, "Balsacone C, a new antibiotic targeting bacterial cell membranes, inhibits clinical isolates of methicillin-resistant Staphylococcus aureus(MRSA)without inducing resistance", Front. Microbiol, 10, pp.2341~ (2019)