A Study on Antimicrobial Activity of Lysimachia clethroides Duby Root Extracts against Methicillin-resistant Staphylococcus aureus
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Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium responsible for a number of infections inhumans that are difficult to treat, and as a result, is a substantial contributor to morbidity and mortality. In the present study,in search of natural products capable of inhibiting this multidrug-resistant bacterium, we investigated the antimicrobialactivity of Lysimachia clethroides Duby root. The antibacterial activities of EtOH extract of Lysimachia clethroides Dubyroot and its n-hexane, EtOAc, n-BuOH and water fractions were evaluated against 15 strains of methicillin-resistantstaphylococcus aureus (MRSA) and 1 standard methicillin-susceptible S. aureus (MSSA) strain by using the minimalinhibitory concentrations (MICs) assay, colorimetric assay using MTT test, checkerboard dilution test. Antimicrobialactivity of n-hexane fraction of Lysimachia clethroides Duby root was remarkable. Against the 16 strains, the minimuminhibitory concentrations (MICs) were in the range of 31.25–62.5 ㎍/㎖ and FICI values for n-hexane fraction ofLysimachia clethroides Duby root+AM and n-hexane fraction of Lysimachia clethroides Duby root+OX were checkerboardmethod performed using the MRSA, MSSA and one clinical isolate strains via MICI 0.12-1 and 0.25-0.75, showing theincrease of synergistic effect. When combined together, these antibiotic effects were dramatically increased. These effectivecombinations could be new promising agents in the management of MRSA.
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium responsible for a number of infections inhumans that are difficult to treat, and as a result, is a substantial contributor to morbidity and mortality. In the present study,in search of natural products capable of inhibiting this multidrug-resistant bacterium, we investigated the antimicrobialactivity of Lysimachia clethroides Duby root. The antibacterial activities of EtOH extract of Lysimachia clethroides Dubyroot and its n-hexane, EtOAc, n-BuOH and water fractions were evaluated against 15 strains of methicillin-resistantstaphylococcus aureus (MRSA) and 1 standard methicillin-susceptible S. aureus (MSSA) strain by using the minimalinhibitory concentrations (MICs) assay, colorimetric assay using MTT test, checkerboard dilution test. Antimicrobialactivity of n-hexane fraction of Lysimachia clethroides Duby root was remarkable. Against the 16 strains, the minimuminhibitory concentrations (MICs) were in the range of 31.25–62.5 ㎍/㎖ and FICI values for n-hexane fraction ofLysimachia clethroides Duby root+AM and n-hexane fraction of Lysimachia clethroides Duby root+OX were checkerboardmethod performed using the MRSA, MSSA and one clinical isolate strains via MICI 0.12-1 and 0.25-0.75, showing theincrease of synergistic effect. When combined together, these antibiotic effects were dramatically increased. These effectivecombinations could be new promising agents in the management of MRSA.
참고문헌 (19)
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