Melanogenesis Inhibitory Activity of Epicatechin-3-OGallate Isolated from Polygonum amphibium L.
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
This study aimed to investigate the melanogenesis inhibitory activity of epicatechin-3-O-gallate (ECG) isolated from Polygonum amphibium L. ECG was isolated from the ethanol extract of P. amphibium L, and its chemical structure was determined using spectroscopic methods such as LC-ESI-MS, 1D-NMR, and UV spectroscopy. ECG inhibited the melanogenesis of B16F10 cells in a dose-dependent manner. Particularly, it decreased the melanin content by 27.4% at 200 μM concentration, compared with the control, in B16F10 cells, without causing cytotoxicity. It is noteworthy that the expression of three key proteins, including tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor (MITF), involved in melanogenesis, is significantly inhibited by ECG. The ECG isolated in this study caused the inhibition of body pigmentation and tyrosinase activity in vivo in the zebrafish model. These results suggest that the ECG isolated from P. amphibium L. is an effective anti-melanogenesis agent.
Abstract
This study aimed to investigate the melanogenesis inhibitory activity of epicatechin-3-O-gallate (ECG) isolated from Polygonum amphibium L. ECG was isolated from the ethanol extract of P. amphibium L, and its chemical structure was determined using spectroscopic methods such as LC-ESI-MS, 1D-NMR, and UV spectroscopy. ECG inhibited the melanogenesis of B16F10 cells in a dose-dependent manner. Particularly, it decreased the melanin content by 27.4% at 200 μM concentration, compared with the control, in B16F10 cells, without causing cytotoxicity. It is noteworthy that the expression of three key proteins, including tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor (MITF), involved in melanogenesis, is significantly inhibited by ECG. The ECG isolated in this study caused the inhibition of body pigmentation and tyrosinase activity in vivo in the zebrafish model. These results suggest that the ECG isolated from P. amphibium L. is an effective anti-melanogenesis agent.
참고문헌 (29)
- Gao Yaqian, "엽록체 DNA 염기서열에 근거한 물여뀌 종집단(마디풀과)의 분류학적 연구", 식물분류학회지, 43(1), pp.34~45 (2013), 한국식물분류학회
- Smolarz HD, "Influence of ethyl acetate extract and quercetin-3-methyl ether from Polygonum amphibium on activation lymphocytes from peripheral blood of healthy donor in vitro", Phytother. Res. PTR, 17, pp.744~747 (2003)
- Smolarz HD, "Flavonoid glucuronides with anti-leukaemic activity from Polygonum amphibium L", Phytochem. Anal : PCA, 19, pp.506~513 (2008)
- 권성호, "수생관속식물의 라디칼 소거능과 남세균 생장에 대한 억제활성 비교", 생태와 환경, 45(1), pp.11~20 (2012), 한국수생태학회
- 황병수, "물여뀌 에탄올 추출물의 미백 효과", 한국미생물·생명공학회지, 47(2), pp.195~200 (2019), 한국미생물·생명공학회[DOI]
- Yun JW, "TRPV1 antagonist can suppress the atopic dermatitis-like symptoms by accelerating skin barrier recovery", J. Dermatol. Sci, 62, pp.8~15 (2011)
- Tsatmali M, "Melanocyte function and its control by melanocortin peptides", J. Histochem. Cytochem, 50, pp.125~133 (2002)
- Briganti S, "Chemical and instrumental approaches to treat hyperpigmentation", Pigment Cell Res, 16, pp.101~110 (2003)
- "Nihon Hifuka Gakkai zasshi. Japanese J. Dermatol", 1991, 609~613
- Fujimoto N, "Induction of thyroid tumours in(C57BL", 6N x C3H, Food Chem. Toxicol, pp.1998, 36[DOI]
- Hermanns JF, "Skin colour assessment in safety testing of cosmetics", An overview. Int. J. Cosmet. Sci, 22, pp.67~71 (2000)
- Davis AL, "1H and 13C NMR assignments of some green tea polyphenols", Magn. Reson. Chem, 34, pp.887~890 (1996)
- Hosoi J, "Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid", Cancer Res, 45, pp.1474~1478 (1985)
- Kimmel CB, "Stages of embryonic development of the zebrafish", Dev. Dyn, 203, pp.253~310 (1995)
- Westerfield M, "The zebrafish book : a guide for the laboratory use of zebrafish (Brachydanio rerio)", pp.385~ (1993)
- Choi TY, "Zebrafish as a new model for phenotype-based screening of melanogenic regulatory compounds", Pigment Cell Res, 20, pp.120~127 (2007)
- Kim YJ, "Tyrosinase inhibitors from natural and synthetic sources : structure, inhibition mechanism and perspective for the future", Cell. Mol. Life Sci, 62, pp.1707~1723 (2005)
- Costin GE, "Human skin pigmentation : melanocytes modulate skin color in response to stress", FASEB J, 21, pp.976~994 (2007)
- Yoon HS, "Involvement of extracellular signal-regulated kinase in nobiletininduced melanogenesis in murine B16", F10 melanoma cells, 2007, pp.71, 1781~1784
- Kim SS, "Down-regulation of tyrosinase, TRP-1, TRP-2 and MITF expressions by citrus press-cakes in murine B16 F10 melanoma", Asian Pac. J. Ttrop. Biomed, 3, pp.617~622 (2013)
- Tuerxuntayi A, "Kaliziri extract upregulates tyrosinase, TRP-1, TRP-2 and MITF expression in murine B16 melanoma cells", BMC Complement. Altern. Med, 14, pp.166~ (2014)
- Zhang X, "Anti-melanogenic effects of epigallocatechin-3-gallate(EGCG), epicatechin-3-gallate(ECG)and gallocatechin-3-gallate(GCG)via down-regulation of cAMP", CREB, Fitoterapia, pp.2020, 145[DOI]
- Lall N, "extract from Ceratonia siliqua exhibits depigmentation properties", Phytother. Res, 29, pp.1729~1736 (2015)
- Fujimaki T, "Isolation of proanthocyanidins from red wine, and their inhibitory effects on melanin synthesis in vitro", Food Chem, 248, pp.61~69 (2018)
- 박수남, "여뀌 추출물의 항산화 및 항노화에 관한 연구", 대한화장품학회지, 35(4), pp.293~300 (2009), 사단법인 대한화장품학회
- Strahle U, "Zebrafish embryos as an alternative to animal experiments--a commentary on the definition of the onset of protected life stages in animal welfare regulations", Reprod. Toxicol, 33, pp.128~132 (2012)
- Stanley KA, "Endosulfan I and endosulfan sulfate disrupts zebrafish embryonic development", Aquat. Toxicol, 95, pp.355~361 (2009)
- O'Reilly-Pol T, "Neocuproine ablates melanocytes in adult zebrafish", Zebrafish, 5, pp.257~264 (2008)
- Lajis AFB, "A zebrafish embryo as an animal model for the treatment of hyperpigmentation in cosmetic dermatology medicine", Medicina, 54, pp.35~ (2018)