The in vitro and in vivo Safety Evaluation of Lactobacillus acidophilus IDCC 3302
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
As consumption of healthy foods continues to garner remarkable public attention, ensuring probiotic safety has become a priority. In this study, the safety of Lactobacillus acidophilus IDCC 3302 was assessed in vitro and in vivo. L. acidophilus IDCC 3302 showed negative results for hemolytic and β-glucuronidase activities. The whole-genome analysis (WGA) revealed that L. acidophilus IDCC 3302 did not possess antibiotic resistance or virulence genes. The minimal inhibitory concentrations of L. acidophilus IDCC 3302 confirmed its safety concerning antibiotic resistance. Furthermore, L. acidophilus IDCC 3302 was demonstrated to be nontoxic in the oral toxicity test in rats. Therefore, the results suggested that L. acidophilus IDCC 3302 might be safe for human consumption.
Abstract
As consumption of healthy foods continues to garner remarkable public attention, ensuring probiotic safety has become a priority. In this study, the safety of Lactobacillus acidophilus IDCC 3302 was assessed in vitro and in vivo. L. acidophilus IDCC 3302 showed negative results for hemolytic and β-glucuronidase activities. The whole-genome analysis (WGA) revealed that L. acidophilus IDCC 3302 did not possess antibiotic resistance or virulence genes. The minimal inhibitory concentrations of L. acidophilus IDCC 3302 confirmed its safety concerning antibiotic resistance. Furthermore, L. acidophilus IDCC 3302 was demonstrated to be nontoxic in the oral toxicity test in rats. Therefore, the results suggested that L. acidophilus IDCC 3302 might be safe for human consumption.
참고문헌 (38)
- Chandan RC, "Enhancing market value of milk by adding cultures", J. Dairy Sci, 82, pp.2245~2256 (1999)
- Gill H, "Probiotics, immunomodulation, and health benefits", Adv. Exp. Med. Biol, 606, pp.423~454 (2008)
- Françoise L, "Occurrence and role of lactic acid bacteria in seafood products", Food Microbiol, 27, pp.698~709 (2010)
- Chamba JF, "Cheese: chemistry, physics and microbiology", pp.191~206 (2004), Elsevier Academic Press
- Alander M, "Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG, after oral consumption", Appl. Environ. Microbiol, 65, pp.351~354 (1999)
- Danielsen M, "Susceptibility of Lactobacillus spp. to antimicrobial agents", Int. J. Food Microbiol, 82, pp.1~11 (2003)
- Kirjavainen PV, "The ability of probiotic bacteria to bind to human intestinal mucus", FEMS Microbiol. Lett, 167, pp.185~189 (1998)
- Kos BVZE, "Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92", J. Appl. Microbiol, 94, pp.981~987 (2003)
- Tannock GW, "Probiotics : a critical review", J. Antimicrob. Chemother, 43, pp.849~ (1999)
- Abriouel H, "New insights in antibiotic resistance of Lactobacillus species from fermented foods", Food Res. Int, 78, pp.465~481 (2015)
- Donohue DC, "Safety of probiotic bacteria", Asia pacific J. Clin. Nutr, 5, pp.25~28 (1996)
- Saxelin M, "Safety of commercial products with viable Lactobacillus strains", Infect. Dis. Clin. Pract, 5, pp.331~335 (1996)
- Antony SJ, "Lactobacillemia : an emerging cause of infection in both the immunocompromised and the immunocompetent host", J. Natl. Med. Assoc, 92, pp.83~86 (2000)
- Schlegel L, "Lactobacillus species as opportunistic pathogens in immunocompromised patients", Eur. J. Clin. Microbiol. Infect. Dis, 17, pp.887~888 (1998)
- Vijayalakshmi S, "Comprehensive in silico analysis of lactic acid bacteria for the selection of desirable probiotics", LWT, 130, pp.109617~ (2020)
- Chen L, "VFDB : a reference database for bacterial virulence factors", Nucl. Acids Res, 33, pp.D325~D328 (2005)
- Zankari E, "Identification of acquired antimicrobial resistance genes", J. Antimicrob. Chemother, 67, pp.2640~2644 (2012)
- Arndt D, "PHASTER : a better, faster version of the PHAST phage search tool", Nucl. Acids Res, 44, pp.W16~W21 (2016)
- Priyadarshani WM, "Screening selected strains of probiotic lactic acid bacteria for their ability to produce biogenic amines(histamine and tyramine)", Int. J. Food Sci. Technol, 46, pp.2062~2069 (2011)
- EFSA Panel on Additives, "Guidance on the characterisation of microorganisms used as feed additives or as production organisms", EFSA J, 16, pp.e05206~ (2018)
- OECD Guideline for the Testing of Chemicals. No. 423: Acute Oral Toxicity — Acute Toxic Class Method, "OECD", Organisation for Economic Co-operation and Development, 14~ (2001)
- Ammor MS, "Antibiotic resistance in nonenterococcal lactic acid bacteria and bifidobacteria", Food Microbiol, 24, pp.559~570 (2007)
- Charteris WP, "Gradient diffusion antibiotic susceptibility testing of potentially probiotic lactobacilli", J. Food Prot, 64, pp.2007~2014 (2001)
- Mayrhofer S, "Antibiotic susceptibility of members of the Lactobacillus acidophilus group using broth microdilution and molecular identification of their resistance determinants", Int. J. Food Microbiol, 144, pp.81~87 (2010)
- Temmerman R, "Identification and antibiotic susceptibility of bacterial isolates from probiotic products", Int. J. Food Microbiol, 81, pp.1~10 (2003)
- Kodner C, "Diagnosis and management of acute interstitial nephritis", Am. Fam. Physician, 67, pp.2527~2534 (2003)
- Kim DH, "Intestinal bacterial β-glucuronidase activity of patients with colon cancer", Arch. Pharm. Res, 24, pp.564~567 (2001)
- Choi YB, "Hydrolysis of soybean isoflavone glucosides by lactic acid bacteria", Biotechnol. Lett, 24, pp.2113~2116 (2002)
- Delgado S, "In vitro evaluation of the probiotic properties of human intestinal Bifidobacterium species and selection of new probiotic candidates", J. Appl. Microbiol, 104, pp.1119~1127 (2008)
- Colombo M, "Beneficial properties of lactic acid bacteria naturally present in dairy production", BMC Microbiol, 18, pp.219~ (2018)
- Spano G, "Biogenic amines in fermented foods", Eur. J. Clin. Nutr, 64, pp.S95~S100 (2010)
- Barbieri F, "Biogenic amine production by lactic acid bacteria : a review", Foods, 8, pp.17~ (2019)
- Ladero V, "Toxicological effects of dietary biogenic amines", Curr. Nutr. Food Sci, 6, pp.145~156 (2010)
- Sanders ME, "Safety assessment of probiotics for human use", Gut Microbes, 1, pp.164~185 (2010)
- Uribarri J, "D-lactic acidosis : a review of clinical presentation, biochemical features, and pathophysiologic mechanism", Medicine, 77, pp.73~82 (1998)
- Møller PD, "Absorption and fate of L-and D-lactic acid in ruminants", Physiol. A Physiol, 118, pp.387~388 (1997)
- Quigley EM, "‘Brain Fogginess’ and DLactic acidosis : probiotics are not the cause", Clin. Transl. Gastroenterol, 9, pp.187~ (2018)
- Sulemankhil I, "In vitro and in vivo characterization and strain safety of Lactobacillus reuteri NCIMB 30253 for probiotic applications", Can. J. Microbiol, 58, pp.776~787 (2012)
