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Melanin Biosynthesis Inhibition Effects of Ginsenoside Rb2 Isolated from Panax ginseng Berry

이재원|정상철|김진희|정용태|이승은|이미경|김금숙|강희철|안영섭|민진우|이대영
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2015|Journal of Microbiology and Biotechnology|제25권 제12호|pp. 2011-2015|DOI 10.4014/jmb.1505.05069

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참고문헌

키워드

Panax ginsengzebrafishGinsenoside Rb2melanin biosynthesismelan-a cell
주제분류생물학

초록

Ginsenoside Rb2 (Gin-Rb2) was purified from the fruit extract of Panax ginseng. Its chemical structure was measured by spectroscopic analysis, including HR-FAB-MS, 1H-NMR, and IR spectroscopy. Gin-Rb2 decreased potent melanogenesis in melan-a cells, with 23.4% at 80 μM without cytotoxicity. Gin-Rb2 also decreased tyrosinase and MITF protein expression in melan-a cells. Furthermore, Gin-Rb2 presented inhibition of the body pigmentation in the zebrafish in vivo system and reduced melanin contents and tyrosinase activity. These results show that Gin-Rb2 isolated from P. ginseng may be an effective skin-whitening agent via the in vitro and in vivo systems.

Abstract

Ginsenoside Rb2 (Gin-Rb2) was purified from the fruit extract of Panax ginseng. Its chemical structure was measured by spectroscopic analysis, including HR-FAB-MS, 1H-NMR, and IR spectroscopy. Gin-Rb2 decreased potent melanogenesis in melan-a cells, with 23.4% at 80 μM without cytotoxicity. Gin-Rb2 also decreased tyrosinase and MITF protein expression in melan-a cells. Furthermore, Gin-Rb2 presented inhibition of the body pigmentation in the zebrafish in vivo system and reduced melanin contents and tyrosinase activity. These results show that Gin-Rb2 isolated from P. ginseng may be an effective skin-whitening agent via the in vitro and in vivo systems.

참고문헌 (31)

  1. 배혜민, "Inhibitory Effects of Ginsenoside Re Isolated from Ginseng Berry on Histamine and Cytokine Release in Human Mast Cells and Human Alveolar Epithelial Cells", Journal of Ginseng Research, 36(4), pp.369~374 (2012), 고려인삼학회
  2. Bennett DC, "A line of nontumorigenic mouse melanocytes, syngeneic with the B16melanoma and requiring a tumour promoter for growth", Int. J. Cancer, 39, pp.414~418 (1987)
  3. Bentley NJ, "Melanocyte-specific expression of the human tyrosinase promoter: activation by the microphthalmia gene product and role of the initiator", Mol. Cell. Biol, 14, pp.7996~8006 (1994)
  4. Briganti S, "Chemical and instrumental approaches to treat hyperpigmentation", Pigment Cell Res, 16, pp.101~110 (2003)
  5. Choi TY, "Zebrafish as a new model for phenotype-based screening of melanogenic regulatory compounds", Pigment Cell Res, 20, pp.120~127 (2007)
  6. Elsalini OA, "Phenylthiourea disrupts thyroid function in developing zebrafish", Dev. Genes Evol, 212, pp.593~598 (2003)
  7. Fujimoto N, "Induction of thyroid tumours in (C57BL", 6N x C3H, Food Chem. Toxicol, pp.1998, 36[DOI]
  8. Gaggioli C, "Microphthalmia-associated transcription factor (MITF) is required but is not sufficient to induce the expression of melanogenic genes", Pigment Cell Res, 16, pp.374~382 (2003)
  9. Huo YS, "Anti-senility action of saponin in Panax ginseng fruit in 327 cases", Chin. J. Modern Dev. Trad. Med, 4, pp.593~596 (1984)
  10. Im SJ, "Effect of Radix Ginseng and Radix Trichosanthis on the melanogenesis", Biol. Pharm. Bull, 26, pp.849~853 (2003)
  11. 김길남, "Whitening Effect of Octaphlorethol A Isolated from Ishige foliacea in an In Vivo Zebrafish Model", Journal of Microbiology and Biotechnology, 25(4), pp.448~451 (2015), 한국미생물·생명공학회[DOI]
  12. 김수진, "Physicochemical Studies of a Newly Synthesized Molecule, 6-Methyl-3- phenethyl-3,4-dihydro-1H-quinazoline-2-thione (JSH18) for Topical Formulations", Archives of Pharmacal Research, 31(10), pp.1363~1368 (2008), 대한약학회
  13. Kong YH, "Inhibitory effects of cinnamic acid on melanin biosynthesis in skin", Biol. Pharm. Bull, 31, pp.946~948 (2008)
  14. Lee DY, "The potential of minor ginsenosides isolated from the leaves of Panax ginseng as inhibitors of melanogenesis", Int. J. Mol. Sci, 16, pp.1677~1690 (2015)
  15. Lee HJ, "In vivo radioprotective effect of Panax ginseng C.A. Meyer and identification of active ginsenosides", Phytother. Res, 20, pp.392~395 (2006)
  16. Kyoung-Tae Lee, "The Antidiabetic Effect of Ginsenoside Rb2 via Activation of AMPK", Archives of Pharmacal Research, 34(7), pp.1201~1208 (2011), 대한약학회
  17. Sato K, "Inhibition of tumor angiogenesis and metastasis by a saponin of Panax ginseng, ginsenoside-Rb2", Biol. Pharm. Bull, 17, pp.635~639 (1994)
  18. Shao ZH, "Antioxidant effects of American ginseng berry extract in cardiomyocytes exposed to acute oxidant stress", Biochim. Biophys. Acta, 1670, pp.165~171 (2004)
  19. Strahle U, "Zebrafish embryos as an alternative to animal experiments - a commentary on the definition of the onset of protected life stages in animal welfare regulations", Reprod. Toxicol, 33, pp.128~132 (2012)
  20. Wang L, "The melanogenesis-inhibitory effect and the percutaneous formulation of ginsenoside Rb1", AAPS PharmSciTech, 15, pp.1252~1262 (2014)
  21. Wang W, "In vitro anti-cancer activity and structure-activity relationships of natural products isolated from fruits of Panax ginseng", Cancer Chemother. Pharmacol, 59, pp.589~601 (2007)
  22. Wu M, "c-Kit triggers dual phosphorylations, which couple activation and degradation of the essential melanocyte factor Mi", Genes Dev, 14, pp.301~312 (2000)
  23. Xie JT, "Effects of American ginseng berry extract on blood glucose levels in ob", ob mice, 2002, pp.30, 187~194
  24. Xie JT, "Antioxidant effects of ginsenoside Re in cardiomyocytes", Eur. J. Pharmacol, 532, pp.201~207 (2006)
  25. Xie JT, "American ginseng berry juice intake reduces blood glucose and body weight in ob", ob mice, 2007, pp.72, S590~S594
  26. Xie JT, "In vitro and in vivo anticancer effects of American ginseng berry: exploring representative compounds", Biol. Pharm. Bull, 32, pp.1552~1558 (2009)
  27. Yasumoto K, "Functional analysis of microphthalmia-associated transcription factor in pigment cell-specific transcription of the human tyrosinase family genes", J. Biol. Chem, 272, pp.503~509 (1997)
  28. 유영춘, "Protective effect of ginsenoside-Rb2 from Korean red ginseng on the lethal infection of haemagglutinating virus of Japan in mice", Journal of Ginseng Research, 37(1), pp.80~86 (2013), 고려인삼학회
  29. Yoon HS, "Involvement of extracellular signal-regulated kinase in nobiletin-induced melanogenesis in murine B16", F10melanoma cells, 2007, pp.71, 1781~1784
  30. Yun JW, "TRPV1 antagonist can suppress the atopic dermatitislike symptoms by accelerating skin barrier recovery", J. Dermatol. Sci, 62, pp.8~15 (2011)
  31. Zhao X, "Highly selective biotransformation of ginsenoside Rb1 to Rd by the phytopathogenic fungus Cladosporium fulvum (syn. Fulvia fulva)", J. Ind. Microbiol. Biotechnol, 36, pp.721~726 (2009)

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