Antibacterial Activity of Streptomyces Strains Isolated from Different Regions of Jordan
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Members of the genus Streptomyces produce more than 70% of antibiotics. The rise in antibiotic resistance globally enhanced the search for novel species with the ability to produce new bioactive compounds. This study was initiated to investigate different regions in Jordan for previously uncultured and rare Streptomyces species capable of producing novel antimicrobial compounds especially active against bacteria resistant to antibiotics. A total of 191 Streptomyces strains were isolated from 26 soil samples collected from different geographic regions in Jordan. Isolates were characterized based on colony and cellular morphology as well as using 16S rRNA gene sequencing. These isolates were screened for their ability to produce antibiotics by the perpendicular-cross streak method, and then tested by well diffusion method against tested pathogens. Fifty-four isolates showed potential to produce antimicrobial products especially active against resistant bacteria, 20.1% of the isolates showed inhibitory effect against Staphylococcus aureus, 16.9% against clinical MSSA strains, and 18.0% against MRSA: whereas only 4.2% against Esherichia coli, 3.2% against Klebsiella pneumonia, 2.7% against Pseudomonas aeruginosa, and 10.0% against clinical Candida albicans. Three isolates were selected for further identification due to their antibacterial activity against S. aureus, MRSA, and MSSA. These isolates were identified as follows; Streptomyces aburaviensis DSa3, Streptomyces alboniger SAb7 and Streptomyces misionensis ZAb2, based on cultural, biochemical characteristics and molecular analysis of the 16S rRNA.
Abstract
Members of the genus Streptomyces produce more than 70% of antibiotics. The rise in antibiotic resistance globally enhanced the search for novel species with the ability to produce new bioactive compounds. This study was initiated to investigate different regions in Jordan for previously uncultured and rare Streptomyces species capable of producing novel antimicrobial compounds especially active against bacteria resistant to antibiotics. A total of 191 Streptomyces strains were isolated from 26 soil samples collected from different geographic regions in Jordan. Isolates were characterized based on colony and cellular morphology as well as using 16S rRNA gene sequencing. These isolates were screened for their ability to produce antibiotics by the perpendicular-cross streak method, and then tested by well diffusion method against tested pathogens. Fifty-four isolates showed potential to produce antimicrobial products especially active against resistant bacteria, 20.1% of the isolates showed inhibitory effect against Staphylococcus aureus, 16.9% against clinical MSSA strains, and 18.0% against MRSA: whereas only 4.2% against Esherichia coli, 3.2% against Klebsiella pneumonia, 2.7% against Pseudomonas aeruginosa, and 10.0% against clinical Candida albicans. Three isolates were selected for further identification due to their antibacterial activity against S. aureus, MRSA, and MSSA. These isolates were identified as follows; Streptomyces aburaviensis DSa3, Streptomyces alboniger SAb7 and Streptomyces misionensis ZAb2, based on cultural, biochemical characteristics and molecular analysis of the 16S rRNA.
참고문헌 (65)
- Williams ST, "Numerical classification of Streptomyces and related genera", J. Gen. Microbiol, 129, pp.1743~1813 (1983)
- Williams ST, "A probability matrix for identification of Streptomyces", J. Gen. Microbiol, 129, pp.1815~1830 (1983)
- Goodfellow M, "The Actinobacteria, part A and B" (2012), Springer
- Salam N, "Update on the classification of higher ranks in the phylum Actinobacteria", Int. J. Syst. Evol. Microbiol, 70, pp.1331~1355 (2020)
- Lacey H, "Recently discovered secondary metabolites from Streptomyces species", Molecules, 27, pp.887~ (2022)
- Ayswaria R, "Diverse endophytic Streptomyces species with dynamic metabolites and their meritorious applications : a critical review", Crit. Rev. Microbiol, 46, pp.750~758 (2020)
- Sivalingam P, "Extreme environment Streptomyces: Potential sources for new antibacterial and anticancer drug leads", Int. J. Microbiol (2019)[DOI]
- Chater KF, "Recent advances in understanding Streptomyces", F1000Res, 5, pp.2795~ (2016)
- Hui M, "The extremophilic actinobacteria : From microbes to medicine", Antibiotics, 10, pp.682~ (2021)
- Jones S, "Streptomyces exploration is triggered by fungal interactions and volatile signals", ELife, 6, pp.e21738~ (2017)
- Humberto EO, "Antifungal compounds from Streptomyces associated with attine ants also inhibit Leishmania donovani", PLoS Negl. Trop. Dis, 13, pp.e0007643~ (2019)
- Harir M, "Basic Biology and Applications of Actinobacteria" (2018), IntechOpen
- Law W, "Diversity of Streptomyces spp. from mangrove forest of Sarawak (Malaysia) and screening of their antioxidant and cytotoxic activities", Sci. Rep, 9, pp.15262~ (2019)
- Fangfang L, "Anti-influenza A viral butenolide from Streptomyces sp. Smu03 inhabiting the intestine of Elephas maximus", Viruses, 10, pp.356~ (2018)
- Bergeijk D, "Ecology and genomics of actinobacteria : new concepts for natural product discovery", Nat. Rev. Microbiol, 18, pp.546~558 (2020)
- Nguyen HT, "Streptomyces sp. VN1, a producer of diverse metabolites including non-natural furan-type anticancer compound", Sci. Rep, 10, pp.1756~ (2020)
- Singh B, "Improving production of tacrolimus in streptomyces tacrolimicus(atcc 55098)through development of novel mutant by dual mutagenesis", Braz. Arch. Biol. Technol, 60, pp.e17160366~ (2017)
- Tran T, "Streptomyces strains promote plant growth and induce resistance against Fusarium verticillioides via transient regulation of auxin signaling and archetypal defense pathways in maize plants", Front. Plant Sci, 12, pp.755733~ (2021)
- Howard T, "The Production of Neomycin by Streptomyces fradiae in synthetic media", Appl. Microbiol, 1, pp.103~106 (1953)
- Akagawa H, "A plasmid involved in chloramphenicol production in Streptomyces venezuelae : Evidence from genetic mapping", J. Gen. Microbiol, 90, pp.336~346 (1975)
- Sankaran L, "Biosynthesis of puromycin in Streptomyces alboniger : regulation and properties of O-demethylpuromycin O-methyltransferase", Antimicrob. Agents Chemother, 8, pp.721~732 (1975)
- Distler J, "Gene cluster for streptomycin biosynthesis in Streptomyces griseus : Nucleotide sequence of three genes and analysis of transcriptional activity", Nucleic Acids Res, 15, pp.8041~8056 (1987)
- Swan DG, "Characterisation of a Streptomyces antibioticus gene encoding a type I polyketide synthase which has an unusual coding sequence", Mol. Gen. Genet, 242, pp.358~362 (1994)
- Peschke U, "Molecular characterization of the lincomycin-production gene cluster of Streptomyces lincolnensis", Mol. Microbiol, 16, pp.1137~1156 (1995)
- Nelson M, "Tetracyclines in biology, chemistry and medicine", Birkhäuser Basel, pp.8~ (2001)[DOI]
- Miao V, "Daptomycin biosynthesis in Streptomyces roseosporus:Cloning and analysis of the gene cluster and revision of peptide stereochemistry", Microbiology, 151(5), pp.1507~1523 (2005)
- Woodyer R, "Heterologous production of Fosfomycin and identification of the minimal biosynthetic gene cluster", Chem. Biol, 13, pp.1171~1182 (2006)
- Atta H, "Biochemical studies on antibiotic production from Streptomyces sp.: Taxonomy, fermentation, isolation and biological property", J. Saudi. Chem. Soc, 19, pp.12~22 (2010)
- Wu C, "Metabolomics-guided analysis of isocoumarins production by Streptomyces species MBT76 and biotransformation of flavonoids and phenylpropanoids", Metabolomics, 12, pp.90~ (2016)
- Xie F, "Actinobacteria from desert : Diversity and biotechnological applications", Front. Microbiol, 12, pp.765531~ (2021)
- "Biogeography, Atlas of Jordan, Publications de l’Institut français du Proche-Orient Contemporary publications | CP 32 Beirut" (2013)[URL]
- Schlatter D, "Resource amendments influence density and competitive phenotypes of Streptomyces in soil", Microb. Ecol, 57, pp.413~420 (2009)
- Law J, "The potential of Streptomyces as biocontrol agents against the rice blast fungus, Magnaporthe oryzae(Pyricularia oryzae)", Front. Microbiol, 8, pp.3~ (2017)
- Saadoun I, "Comparative distribution of soil Streptomyces flora in different Jordanian habitats and their enzymatic and antibiotic activities", Res. J. Pharm. Biol. Chem. Sci, 8, pp.1285~1297 (2017)
- Suneetha V, "Isolation and identification of Streptomyces ST1 and ST2 strains from Tsunami affected soils : Morphological and biochemical studies", J. Oceanogr. Mar. Sci, 2, pp.96~101 (2011)
- Saadoun I, "Characterization and analysis of antibiotic activity of some aquatic actinomycetes", Microbios, 99, pp.173~179 (1999)
- Kuster E, "Selection of media for isolation of Streptomycetes", Nature, 202, pp.928~929 (1964)
- Shirling E, "Methods, classification, identification and description of genera and species", The Williams and Wilkins Company, Baltimore, 2, pp.61~292 (1966)
- Dal Pizzol M, "Antifungal efficacy and safety of cycloheximide as a supplement in optisol-GS", Drug Des. Devel. Ther, 15, pp.2091~2098 (2021)
- Oskay M, "Comparison of Streptomyces diversity between agricultural and non-agricultural soils by using various culture media", Sci. Res. Essay, 4, pp.997~1005 (2009)
- Madigan MT, "Brock Biology of Microorganisms" (1997), Prentice Hall International, Inc
- Le Page S, "Evaluation of the PREVI® Isola automated seeder system compared to reference manual inoculation for antibiotic susceptibility testing by the disk diffusion method", Eur. J. Clin. Microbiol. Infect. Dis, 34, pp.1859~1869 (2015)
- Shirling E, "Methods for characterization of Streptomyces species", Int. J. Syst. Evol. Microbiol, 16, pp.313~340 (1966)
- Saadoun I, "Studies on soil Streptomycetes from Jordan", Actinomycetes, 8, pp.42~48 (1997)
- Saadoun I, "The Streptomyces flora of Jordan and its’ potential as a source of antibiotics active against antibiotic-resistant Gram-negative bacteria", World J. Microbiol. Biotechnol, 18, pp.465~470 (2002)
- Saadoun I, "The Streptomyces flora of Badia region of Jordan and its potential as a source of antibiotic-resistant bacteria", J. Arid. Environ, 53, pp.365~371 (2003)
- Saadoun I, "Isolation, identification and analysis of antibacterial activity of soil Streptomycetes isolates from north Jordan", Microbios, pp.100~ (1999)[DOI]
- Saadoun I, "Characterization and analysis of antifungal activity of soil Streptomycetes isolated from North Jordan", Egypt J. Microbiol, 35, pp.463~471 (2000)
- Saadoun I, "Optimal production conditions, extraction, partial purification and characterization of inhibitory compound(s)produced by Streptomyces Ds-104 isolate against multi-drug resistant Candida albicans, Curr", Trends Biotechnol. Pharm, 2, pp.402~420 (2008)
- Kouadri F, "Antimicrobial activity of Streptomyces sp. isolated from the gulf of Aqaba-Jordan and screening for NRPS, PKS-I, and PKS-II genes", Afr. J. Biotechnol, 13, pp.3505~3515 (2014)
- Rawashdeh R, "Effect of cultural conditions on xylanase production by Streptomyces sp. (strain Ib 24D) and its potential to utilize tomato pomace Afr", J. Biotechol, 4, pp.251~255 (2005)
- Martínez-Castro M, "Streptomyces tacrolimicus sp. nov., a low producer of the immunosuppressant tacrolimus (FK506)", Int. J. Syst. Evol. Microbiol, 61, pp.1084~1088 (2011)
- Yao JY, "Anti-parasitic activities of specific bacterial extracellular products of Streptomyces griseus SDX-4 against Ichthyophthirius multifiliis", Parasitol. Res, 113, pp.3111~3117 (2014)
- Abd-Elnaby H, "Antibacterial and anticancer activity of marine Streptomyces parvus : Optimization and application", Biotechnol. Biotechnol. Equip, 30, pp.180~191 (2016)
- Silambarasan S, "Antibacterial and antifungal activities of actinobacteria isolated from Rathnagiri hills", J. Appl. Pharm. Sci, 2, pp.99~103 (2012)
- Valli S, "Antimicrobial potential of Actinomycetes species isolated from marine environment", Asian Pac. J. Trop. Biomed, 2, pp.469~473 (2012)
- Al-Ansari M, "Antimicrobial potential of Streptomyces sp. to the Gram positive and Gram-negative pathogens", J. Infect. Public Health, 12, pp.861~866 (2019)
- Jalal K, "Molecular and phenotypic characterization of novel Streptomyces species isolated from Kurdistan soil and its antibacterial activity against human pathogens", Jordan J. Biol. Sci, 14, pp.441~451 (2021)
- Meliani M, "Characterization of Actinomycetes strains isolated from Cheliff Estuary in the North-West of Algeria", Jordan J. Biol. Sci, 15, pp.7~14 (2022)
- Tenebro CP, "Multiple strain analysis of Streptomyces species from Philippine marine sediments reveals intraspecies heterogeneity in antibiotic activities", Sci. Rep, 11, pp.17544~ (2021)
- Magaldi S, "Well diffusion for antifungal susceptibility testing", Int. J. Infect. Dis, 8, pp.39~45 (2004)
- Valgas C, "Screening methods to determine antibacterial activity of natural products", Braz. J. Microbiol, 38, pp.369~380 (2007)
- Thumar JT, "Isolation and partial purification of an antimicrobial agent from halotolerant alkaliphilic Streptomyces aburaviensis strain Kut-8", World. J. Microbiol. Biotechnol, 26, pp.2081~2087 (2010)
- Vara J, "Biosynthesis of puromycin by Streptomyces alboniger : Characterization of puromycin N-acetyltransferase", Biochemistry, 24, pp.8074~8081 (1985)
- Yoon BD, "Microbial preparation, containing Streptomyces misonensis CJS-70, for resisting plant pathogens", Republic of Korea, KR2009129181 A 2009-12-16, ~ (2009)
