Therapeutic Prospects of Actinomycetes Derived Metabolites Isolated from Coastal Sediments
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Marine actinomycetes have gained increasing attention as major producers of bioactive secondary metabolites with potential therapeutic applications. In this study, five actinomycetes strains were isolated from marine sediment samples and identified based on morphological characteristics and 16S rRNA sequencing. The identified strains were Streptomyces olivoverticillatus (T-2), Streptomyces cyaneus (T-4), Nocardiopsis synnemataformans (T-7), Streptomyces albogriseolus (T-8), and Streptomyces atrovirens (T-9). These strains were cultured in Starch Casein Broth, and their extracts were evaluated for antibacterial, antioxidant, anticoagulant, and anti-inflammatory activities. Among the tested strains, T-4 and T-8 exhibited significant antimicrobial activity, with T-4 showing the lowest minimum inhibitory concentration (MIC) of 0.1 mg/ml against Vibrio cholerae. Notably, T-8 demonstrated strong antioxidant potential, as indicated by its DPPH radical scavenging activity (IC50 = 372.09 ± 11.05 µg/ml), and potent anti-inflammatory effects, stabilizing red blood cell membranes with an IC50 of 140.08 ± 2.30 µg/ml. Additionally, T-9 exhibited anticoagulant activity with a prolonged prothrombin time (12.08 ± 1.46 min) and trypsin inhibition (IC50 = 435.12 ± 15.88 µg/ml). These findings highlight the significant therapeutic potential of marine sediment-derived actinomycetes and support further research as possible sources of novel bioactive compounds.
Abstract
Marine actinomycetes have gained increasing attention as major producers of bioactive secondary metabolites with potential therapeutic applications. In this study, five actinomycetes strains were isolated from marine sediment samples and identified based on morphological characteristics and 16S rRNA sequencing. The identified strains were Streptomyces olivoverticillatus (T-2), Streptomyces cyaneus (T-4), Nocardiopsis synnemataformans (T-7), Streptomyces albogriseolus (T-8), and Streptomyces atrovirens (T-9). These strains were cultured in Starch Casein Broth, and their extracts were evaluated for antibacterial, antioxidant, anticoagulant, and anti-inflammatory activities. Among the tested strains, T-4 and T-8 exhibited significant antimicrobial activity, with T-4 showing the lowest minimum inhibitory concentration (MIC) of 0.1 mg/ml against Vibrio cholerae. Notably, T-8 demonstrated strong antioxidant potential, as indicated by its DPPH radical scavenging activity (IC50 = 372.09 ± 11.05 µg/ml), and potent anti-inflammatory effects, stabilizing red blood cell membranes with an IC50 of 140.08 ± 2.30 µg/ml. Additionally, T-9 exhibited anticoagulant activity with a prolonged prothrombin time (12.08 ± 1.46 min) and trypsin inhibition (IC50 = 435.12 ± 15.88 µg/ml). These findings highlight the significant therapeutic potential of marine sediment-derived actinomycetes and support further research as possible sources of novel bioactive compounds.