Bioactivity of Metabolites from Actinomycetes Isolates from Red Sea, Egypt
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Actinomycetes isolated from marine habitats represent a promising source of bioactive substances. Here, we report on the isolation, identification, productivity enhancement and application of the bioactive compounds of Streptomyces qinglanensis H4. Eighteen marine actinomycetes were isolated and tested for resistance to seven bacterial diseases. Using 16S rRNA sequencing analysis (GenBank accession number MW563772), the most powerful isolate was identified as S. qinglanensis. Although the strain produced active compound(s) against a number of Gram-negative and Gram-positive bacteria, it failed to inhibit pathogenic fungi. The obtained inhibition zones were 22.0 ± 1.5, 20.0 ± 1, 16.0 ± 1, 12.0 ± 1, 22.0 ± 1 and 24.0 ± 1 mm against Bacillus subtilis ATCC 6633, Escherichia coli ATCC 19404, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 9027, Candida albicans ATCC 10231 and Staphylococcus aureus ATCC6538, respectively. To maximize bioactive compound synthesis, the Plackett-Burman design was used. The productivity increased up to 0.93-fold, when S. qinglanensis was grown in optimized medium composed of: (g/l) starch 30; KNO3 0.5; K2HPO4 0.25; MgSO4 0.25; FeSO4·7H2O, 0.01; sea water concentration (%) 100; pH 8.0, and an incubation period of 9 days. Moreover, the anticancer activity of S. qinglanensis was tested against two different cell lines: HepG2 and CACO. The inhibition activities were 42.96 and 57.14%, respectively. Our findings suggest that the marine S. qinglanensis strain, which grows well on tailored medium, might be a source of bioactive substances for healthcare companies.
Abstract
Actinomycetes isolated from marine habitats represent a promising source of bioactive substances. Here, we report on the isolation, identification, productivity enhancement and application of the bioactive compounds of Streptomyces qinglanensis H4. Eighteen marine actinomycetes were isolated and tested for resistance to seven bacterial diseases. Using 16S rRNA sequencing analysis (GenBank accession number MW563772), the most powerful isolate was identified as S. qinglanensis. Although the strain produced active compound(s) against a number of Gram-negative and Gram-positive bacteria, it failed to inhibit pathogenic fungi. The obtained inhibition zones were 22.0 ± 1.5, 20.0 ± 1, 16.0 ± 1, 12.0 ± 1, 22.0 ± 1 and 24.0 ± 1 mm against Bacillus subtilis ATCC 6633, Escherichia coli ATCC 19404, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 9027, Candida albicans ATCC 10231 and Staphylococcus aureus ATCC6538, respectively. To maximize bioactive compound synthesis, the Plackett-Burman design was used. The productivity increased up to 0.93-fold, when S. qinglanensis was grown in optimized medium composed of: (g/l) starch 30; KNO3 0.5; K2HPO4 0.25; MgSO4 0.25; FeSO4·7H2O, 0.01; sea water concentration (%) 100; pH 8.0, and an incubation period of 9 days. Moreover, the anticancer activity of S. qinglanensis was tested against two different cell lines: HepG2 and CACO. The inhibition activities were 42.96 and 57.14%, respectively. Our findings suggest that the marine S. qinglanensis strain, which grows well on tailored medium, might be a source of bioactive substances for healthcare companies.
참고문헌 (51)
- Alkershi A, "Phytoplankton in polluted waters of the Red Sea coast of Yemen", J. Mar. Biol. Ass. India, 53, pp.161~166 (2011)
- Hameş-Kocabaş EE, "Isolation strategies of marinederived actinomycetes from sponge and sediment samples", J. Microbiol. Methods, 88, pp.342~347 (2012)
- Bukhari M, "Developments in Sustainable Chemical and Bioprocess Technology", pp.203~210 (2013), Springer
- Cheng C, "Biodiversity, anti-trypanosomal activity screening, and metabolomic profiling of actinomycetes isolated from Mediterranean sponges", PLoS One, 10, pp.e0138528~ (2015)
- Abdelmohsen UR, "Diversity, abundance and natural products of marine sponge-associated actinomycetes", Nat. Prod. Rep, 31, pp.381~399 (2014)
- Abdelmohsen UR, "Actinomycetes from Red Sea sponges : sources for chemical and phylogenetic diversity", Mar. Drugs, 12, pp.2771~2789 (2014)
- Grkovic T, "Two new antioxidant actinosporin analogues from the calcium alginate beads culture of sponge-associated Actinokineospora sp. strain EG49", Bioorg. Med. Chem. Lett, 24, pp.5089~5092 (2014)
- Hamed MM, "Antimicrobial activity of marine actinomycetes and the optimization of culture conditions for the production of antimicrobial agent (s)", J. Pure Appl. Microbiol, 13, pp.2177~2188 (2019)
- Valli S, "Antimicrobial potential of Actinomycetes species isolated from marine environment", Asian Pac. J. Trop. Biomed, 2, pp.469~473 (2012)
- Abdelfattah MS, "Isolation and characterization of marine-derived actinomycetes with cytotoxic activity from the Red Sea coast", Asian Pac. J. Trop. Biomed, 6, pp.651~657 (2016)
- Risan MH, "Isolation, characterization and antibacterial activity of a Rare Actinomycete: Saccharopolyspora sp. In Iraq", ~ (2019)
- Sarika K, "Antimicrobial and antifungal activity of soil actinomycetes isolated from coal mine sites", Saudi J. Biol. Sci, 28, pp.3553~3558 (2021)
- Cizeikiene D, "Antimicrobial activity of lactic acid bacteria against pathogenic and spoilage microorganism isolated from food and their control in wheat bread", Food Control, 31, pp.539~545 (2013)
- Shirling ET, "Methods for characterization of Streptomyces species1", Int. J. Syst. Evol. Microbiol, 16, pp.313~340 (1966)
- David HA, "The paired t test under artificial pairing", Am. Stat, 51, pp.9~12 (1997)
- Pudi N, "Studies on optimization of growth parameters for enhanced production of antibiotic alkaloids by isolated marine actinomycetes", J. Appl. Pharm. Sci, 6, pp.181~188 (2016)
- Sengupta S, "Antimicrobial activities of actinomycetes isolated from unexplored regions of Sundarbans mangrove ecosystem", BMC Microbiol, 15, pp.1~16 (2015)
- Balachandar R, "Synthesis of bioactive compounds from vermicast isolated actinomycetes species and its antimicrobial activity against human pathogenic bacteria", Microb. Pathog, 121, pp.155~165 (2018)
- Gomha SM, "Synthesis and anticancer activities of thiazoles, 1, 3-thiazines, and thiazolidine using chitosan-grafted-poly(vinylpyridine)as basic catalyst", Chem. Heterocycl. Compd, 91, pp.1227~1243 (2015)
- Naorungrote S, "Actinomycetes producing anti-methicillin resistant Staphylococcus aureus from soil samples in Nakhon Si Thammarat", Walailak J. Sci. Technol, 8, pp.131~138 (2011)
- Shinde U, "Membrane stabilizing activity-a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil", Fitoterapia, 70, pp.251~257 (1999)
- george w. Snedecor and william g. Cochran, "Statistical methods", pp.1989, Snedecor G
- Behzad H, "Metagenomic studies of the Red Sea", Gene, 576, pp.717~723 (2016)
- Hamed M, "Distribution and characterization of actinomycetes in mangrove habitats(Red Sea, Egypt)with special emphasis on Streptomyces mutabilis M3MT483919", J. Pure Appl. Microbiol, 15, pp.246~261 (2021)
- Blunt JW, "Marine natural products", Nat. Prod. Rep, 32, pp.116~211 (2015)
- Lu S, "Novel bioactive polyketides isolated from marine actinomycetes : an update review from 2013 to 2019", Chem. Biodivers, 17, pp.e2000562~ (2020)
- Patel JD, "Dynamism of antimicrobial activity of actinomycetes-a case study from undisturbed microbial niche", Adv. Microbiol, 4, pp.45817~ (2014)
- Abd-Elnaby H, "Antibacterial and anticancer activity of marine Streptomyces parvus : optimization and application", Biotechnol. Biotechnol. Equip, 30, pp.180~191 (2016)
- Mustafa GA, "Egypt’s Red Sea coast : phylogenetic analysis of cultured microbial consortia in industrialized sites", Front. Microbiol, 5, pp.363~ (2014)
- Fahmy NM, "Isolation and characterization of Streptomyces sp. NMF76 with potential antimicrobial activity from mangrove sediment, Red Sea, Egypt", Egyptian J. Aquatic. Biol. Fisheries, 24, pp.479~495 (2020)
- Selim MSM, "Secondary metabolites and biodiversity of actinomycetes", J. Genet. Eng. Biotechnol, 19, pp.72~ (2021)
- Xu D, "Bioactive molecules from mangrove Streptomyces qinglanensis 172205", Mar. Drugs, 18, pp.255~ (2020)
- Hassan SWM, "Statistical optimization and valuable applications of bioactive compounds produced by marine Pseudoalteromonas piscicida", J. Appl. Pharm. Sci, 7, pp.084~093 (2017)
- Pal D, "Optimization of medium composition to increase the expression of recombinant human interferon-β using the Plackett-Burman and central composite design in E. coli SE1", 3 Biotech, 11, pp.1~10 (2021)
- Wefky S, "Optimization of fermentation conditions for bioactive compounds production by marine bacterium Enterococcus faecium", J. Appl. Sci. Res, 5, pp.1445~1454 (2009)
- Aliero AA, "Molecular characterization and optimization of bioactive compounds production of three Actinomycetes spp isolated from waste dump soil from Western Uganda", Curr. Trends Biotechnol. Pharm, 12, pp.230~244 (2018)
- El-Sersy NA, "Antagonistic effect of marine Nocardia brasiliensis against the fish pathogen Vibrio damsela : Application of Plackett-Burman experimental design to evaluate factors affecting the production of the antibacterial agent", Int. J. Ocean. Oceanography, 1, pp.141~150 (2006)
- Tresner H, "Differential tolerance of streptomycetes to sodium chloride as a taxonomic aid", Appl. Microbiol, 16, pp.1134~1136 (1968)
- Akond MA, "Effect of temperature, pH and NaCl on the isolates of Actinomycetes from straw and compost samples from Savar, Dhaka, Bangladesh", Am. J. Microbiol. Immunol, 1, pp.10~15 (2016)
- Akond MA, "Role of carbon sources and heavy metals on growth and nitrogen fixing potential of Azospirillum and the effect of Azospirillum strains on vegetative growth of rice", J. Environ. Sci, 3, pp.1~8 (2005)
- Khalifa SA, "Marine natural products : A source of novel anticancer drugs", Mar. Drugs, 17, pp.491~ (2019)
- Yip WK, "Antiproliferative and anti-invasive properties of a purified fraction from Streptomyces sp. H7372", Int. J. Oncol, 37, pp.1229~1241 (2010)
- Procópio REdL, "Antibiotics produced by Streptomyces", Braz. J. Infect. Dis, 16, pp.466~471 (2012)
- Law JW-F, "Anticancer drug discovery from microbial sources : the unique mangrove streptomycetes", Molecules, 25, pp.5365~ (2020)
- Fahmy NM, "Isolation and characterization of marine sponge-associated Streptomyces sp. NMF6 strain producing secondary metabolite (s) possessing antimicrobial, antioxidant, anticancer, and antiviral activities", J. Genet. Eng. Biotechnol, 19, pp.102~ (2021)
- Xu D, "PreQ0base, an unusual metabolite with anti-cancer activity from Streptomyces qinglanensis 172205", Anti-Cancer Agents Med. Chem, 15, pp.285~290 (2015)
- Yoganandam GP, "Evaluation of antiinflammatory and membrane stabilizing properties of various extracts of Punica granatum L.(Lythraceae)", Int. J. PharmTech Res, 2, pp.1260~1263 (2010)
- Pooja S, "Bioactive compounds from marine Streptomyces sp. VITPSA as therapeutics", Front. Biol, 12, pp.280~289 (2017)
- Thosar A, "Marine Streptomyces sp. VITASP as a source of new bioactive secondary metabolites", Curr. Bioactive Compounds, 16, pp.611~617 (2020)
- Girão M, "Actinobacteria isolated from Laminaria ochroleuca : a source of new bioactive compounds", Front. Microbiol, 10, pp.683~ (2019)
- Dholakiya RN, "Antibacterial and antioxidant activities of novel actinobacteria strain isolated from Gulf of Khambhat, Gujarat", Front. Microbiol, 8, pp.2420~ (2017)