Actinobacteria from Cow Feces: Isolation, Identification and Screening for Industrially Important Secondary Metabolites
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Actinobacterial strains isolated from Cow feces were studied for their antifungal attributes against phytopathogens and industrially important enzymes. A total of 30 Actinobacterial strains were obtained from 10 samples of cow feces. All the strains were belonging to the genera Streptomyces on the basis of morphological and chemotaxonomic analysis. During preliminary screening, out of 30 strains, 15 strains (50%) showed antifungal activity against five fungal phytopathogens including Aspergillus niger, Fusarium solani, Fusarium oxysporum, Macrophomina phaseolina and Rhizoctonia solani. While, isolate GBTCF-26 was found to be most active against R. solani with 62.2% inhibition of fungal mycelium, GBTCF-09 was prominent against F. solani and F. oxysporum with percent inhibition of 61.1% and 58.8%, respectively. Out of 30 strains, 19 (63.3%), 16 (53.3%), 11 (36.7%), 10 (33.3%), 4 (13.3%) and 8 (26.7%) strains were producing amylase, caseinase, gelatinase, lipase, chitinase and cellulose, respectively. The selected strains, GBTCF-09, GBTCF- 21 and GBTCF-26, were identified as Streptomyces sp. on the basis of their 16S rDNA sequence. The study supports the idea that the Actinobacteria from unique niches (Cow feces) possess the production potential of industrially important enzymes including bioactive molecules.
Abstract
Actinobacterial strains isolated from Cow feces were studied for their antifungal attributes against phytopathogens and industrially important enzymes. A total of 30 Actinobacterial strains were obtained from 10 samples of cow feces. All the strains were belonging to the genera Streptomyces on the basis of morphological and chemotaxonomic analysis. During preliminary screening, out of 30 strains, 15 strains (50%) showed antifungal activity against five fungal phytopathogens including Aspergillus niger, Fusarium solani, Fusarium oxysporum, Macrophomina phaseolina and Rhizoctonia solani. While, isolate GBTCF-26 was found to be most active against R. solani with 62.2% inhibition of fungal mycelium, GBTCF-09 was prominent against F. solani and F. oxysporum with percent inhibition of 61.1% and 58.8%, respectively. Out of 30 strains, 19 (63.3%), 16 (53.3%), 11 (36.7%), 10 (33.3%), 4 (13.3%) and 8 (26.7%) strains were producing amylase, caseinase, gelatinase, lipase, chitinase and cellulose, respectively. The selected strains, GBTCF-09, GBTCF- 21 and GBTCF-26, were identified as Streptomyces sp. on the basis of their 16S rDNA sequence. The study supports the idea that the Actinobacteria from unique niches (Cow feces) possess the production potential of industrially important enzymes including bioactive molecules.
참고문헌 (36)
- Fushimi S, "Studies on new phosphate ester antifungal antibiotics phoslactomycins I. Taxonomy, fermentation, purification and biological activities", J. Antibiot., 42, pp.1019~1025 (1989)[DOI]
- Tomita K, "Pradimicins A, B and C: new antifungal antibiotics I. Taxonomy, production, isolation and physico-chemical properties", J. Antibiot., 43, pp.755~762 (1989)
- Berdy J, "Bioactive microbial metabolites", J. Antibiot., 58, pp.1~ (2005)[DOI]
- Jensen PR, "Marine actinomycete diversity and natural product discovery", Antonie Van Leeuwenhoek., 87, pp.43~48 (2005)[DOI]
- Gupta KK, "Current status of Cow Dung as a bioresource for sustainable development", Bioresour. Bioprocess., 3, pp.28~ (2016)[DOI]
- A.B.Basak, "In Vitro Inhibitory Activity of Cow Urine and Dung to Fusarium solani f. sp. Cucurbitae", Mycobiology, 30(1), pp.51~54 (2002), 한국균학회
- A.B.Basak, "Inhibitive Activity of Cow Urine and Cow Dung against Sclerotinia sclerotiorum of Cucumber", Mycobiology, 30(3), pp.175~179 (2002), 한국균학회
- Waziri M, "Physicochemical properties and antimicrobial activity of evaporated extract of Cow Dung against some pathogens", J. Sci. Res., 5, pp.135~141 (2012)
- Swain MR, "Biocontrol and other beneficial activities of Bacillus subtilis isolated from cowdung microflora", Microbiol. Res., 164, pp.121~130 (2009)[DOI]
- Chauhan RS, "Panchgavya Therapy (Cowpathy) : Current status and future directions", The Indian Cow: The Scientific and Econmic Journal, 1, pp.3~7 (2004)
- Dhama K, "Panchgavya therapy (Cowpathy) in safeguarding health of animals and humans - a review", Res. Opin. Anim. Vet. Sci., 3, pp.170~178 (2013)
- Radha TK, "Plant growth promoting bacteria from Cow Dung based biodynamic preparations", Ind. J. Microbiol., 54, pp.413~418 (2014)[DOI]
- Kumar V, "Actnomycetes from solitary wasp mud nest and swallow bird mud nest: isolation and screening for their antibacterial activity", World J. Microbiol. Biotechnol., 28, pp.871~880 (2012)[DOI]
- Shirling EB, "Methods for characterization of Streptomyces species", Int. J. Syst. Bacteriol., 16, pp.313~340 (1966)[DOI]
- Locci R, "Bergey's Manual of Systematic Bacteriology, vol 4", pp.2451~06 (1989), Williams and Wilkins
- Staneck JL, "Simplified approach to identification of aerobic Actinomycetes by thin layer chromatography", Appl. Microbiol., 28, pp.226~231 (1974)
- Kimura M, "A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences", J. Mol. Evol., 16, pp.111~120 (1980)[DOI]
- Saitou N, "The neighbor-joining method: A new method for reconstructing phylogenetic trees", Mol. Biol. Evol., 4, pp.406~425 (1987)
- Felsenstein J, "Confidence limits on phylogenies: An approach using the bootstrap", Evolution, 39, pp.783~791 (1985)[DOI]
- Tamura K, "MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods", Mol. Biol. Evol., 28, pp.2731~2739 (2011)[DOI]
- Egorov NS, "Antibiotics: A Scientific Approach", pp.336~337 (1985), Mir Publishers
- Saima MK, "Isolation of novel chitinolytic bacteria and production optimization of extracellular chitinase", J. Genet. Eng. Biotechnol., 11, pp.39~46 (2013)[DOI]
- Aneja KR, "Experiments in Microbiology, Plant Pathology and Biotechnology" (2004), New age international publishers
- Hou CT, "Screening of lipase activities with cultures from the agricultural research services culture collection", J. Am. Oil Chem. Soc., 69, pp.1088~1097 (1992)
- Bevan P, "Identifying small-molecule lead compounds: The screening approach to drug discovery", Trends Biotechnol, 13, pp.115~121 (1995)[DOI]
- Shadomy S, "Recent trends in the discovery, development andevaluation ofantifungal agents", pp.8~14 (1987), Prous Science
- Tan H, "Isolation and characterization of actinomycetes from healthy goat faeces", Lett. Appl. Microbiol., 49, pp.248~253 (2009)[DOI]
- Kumar V, "Taxonomy and antimicrobial activity of moderately salt-tolerant and alkaliphilic Streptomyces sp. MN 9(V) isolated from solitary wasp mud nest", Ann. Microbiol., 62, pp.979~985 (2012)[DOI]
- Wright ES, "DECIPHER: A searchbased approach to chimera identification for 16S rRNA sequences", Appl. Environ. Microbiol., 78, pp.717~725 (2012)
- Isik K, "Molecular identification of different actinomycetes isolated from east black sea plateau soil by 16SrDNA gene sequencing", Afr. J. Microbiol. Res., 8, pp.878~887 (2014)
- Kaur M, "Endophytic actinomycetes from Azadirachta indica A. Juss. Characterization and anti-microbial activity", International J.Adv. Res., 4, pp.676~684 (2016)[DOI]
- Andreolli M, "Diversity of bacterial endophytes in 3 and 15-year-old grapevines of Vitis vinifera cv. Corvina and their potential for plant growth promotion and phytopathogen control", Microbiol. Res., 183, pp.42~52 (2016)[DOI]
- Sharma AD, "Inulinase production using garlic (Allium sativum) powder as a potential substrate in Streptomyces sp", J. Food Eng., 77, pp.1~6 (2005)
- Sharma P, "Screening of xylanase activity of Streptomyces albidoflavus PSM-3n isolated from Uttarakhand", EurAsian J. Biosci., 7, pp.30~40 (2013)
- Nagpure A, "Chitinases: in agriculture and human healthcare", Critical Rev. Biotechnol., 34, pp.215~232 (2014)[DOI]
- Singh SP, "Evaluation of antagonistic and plant growth promoting activities of chitinolytic endophytic actinomycetes associated with medicinal plants against Sclerotium rolfsii in chickpea", J. Appl. Microbiol., 121, pp.506~518 (2016)[DOI]
