Complete Genome Sequence of Escherichia coli - Specific Phage KFS-EC1 Isolated from a Slaughterhouse
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Escherichia coli-specific phage, KFS-EC1, was isolated and purified from a slaughterhouse. The complete genome of the phage was obtained using Illumina MiSeq platforms. Its assembled genome consisted of a single chromosome of 164,715 bp with a GC content of 40.5%. The phage genome contained 170 hypothetical and 101 functional ORFs, and exhibited orthologous average nucleotide identity values of >95% with other E. coli phages belonging to the family Straboviridae. Additionally, phylogenetic analysis revealed that KFSEC1 was finally classified into the family Straboviridae of the genus Caudoviricetes. The genome has been deposited in GenBank under the accession number NC_055757.1.
Abstract
Escherichia coli-specific phage, KFS-EC1, was isolated and purified from a slaughterhouse. The complete genome of the phage was obtained using Illumina MiSeq platforms. Its assembled genome consisted of a single chromosome of 164,715 bp with a GC content of 40.5%. The phage genome contained 170 hypothetical and 101 functional ORFs, and exhibited orthologous average nucleotide identity values of >95% with other E. coli phages belonging to the family Straboviridae. Additionally, phylogenetic analysis revealed that KFSEC1 was finally classified into the family Straboviridae of the genus Caudoviricetes. The genome has been deposited in GenBank under the accession number NC_055757.1.
참고문헌 (10)
- Kim S-H, "Isolation, characterization, and application of a novel, lytic phage vB_SalA_KFSST3 with depolymerase for the control of Salmonella and its biofilm on cantaloupe under cold temperature", Food Res. Int, 172, pp.113062~ (2023)
- Oliveira H, "Genomic diversity of bacteriophages infecting the genus acinetobacter", Viruses, 14, pp.181~ (2022)
- Turner D, "Abolishment of morphology-based taxa and change to binomial species names : 2022 taxonomy update of the ICTV bacterial viruses subcommittee", Arch. Virol, 168, pp.74~ (2023)
- Lee C, "Isolation and characterization of a novel Escherichia coli O157 : H7-specific phage as a biocontrol agent", J. Environ. Health Sci. Eng, 18, pp.189~199 (2020)
- Aziz RK, "The RAST Server : rapid annotations using subsystems technology", BMC Genomics, 9, pp.75~ (2008)
- Lee I, "OrthoANI : an improved algorithm and software for calculating average nucleotide identity", Int. J. Syst. Evol. Microbiol, 66, pp.1100~1103 (2016)
- Meier-Kolthoff JP, "VICTOR : genome-based phylogeny and classification of prokaryotic viruses", Bioinformatics, 33, pp.3396~3404 (2017)
- Bailly-Bechet M, "Causes for the intriguing presence of tRNAs in phages", Genome Res, 17, pp.1486~1495 (2007)
- Abeysekera GS, "Bacteriophage-encoded lethal membrane disruptors : Advances in understanding and potential applications", Front. Microbiol, 13, pp.1044143~ (2022)
- Knecht LE, "Diversity and function of phage encoded depolymerases", Front. Microbiol, 10, pp.2949~ (2020)