Complete Genome Sequence of Salmonella Typhimurium-Specific Phage vB_SalA_KFSST3 Possessing Antibiofilm Activity
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
In a previous study, Salmonella Typhimurium-specific phage vB_SalA_KFSST3, which possess antibiofilm activity, was isolated and purified from wastewater used in slaughterhouses. This study aimed to perform bioinformatic analyses to investigate the genes associated with its antibiofilm activity. Phage genome consisted of a single chromosome of 156,555 bp with a GC content of 44.8%. Among its 202 open reading frames (ORFs), three tail spike proteins (TSPs; orf141, orf142, orf143) were identified with high confidence. All TSPs were predicted to encode putative depolymerase activities, including two endoglycosidases and one endorhamnosidase. The genome has been deposited in GenBank under the accession number PP_994976.1.
Abstract
In a previous study, Salmonella Typhimurium-specific phage vB_SalA_KFSST3, which possess antibiofilm activity, was isolated and purified from wastewater used in slaughterhouses. This study aimed to perform bioinformatic analyses to investigate the genes associated with its antibiofilm activity. Phage genome consisted of a single chromosome of 156,555 bp with a GC content of 44.8%. Among its 202 open reading frames (ORFs), three tail spike proteins (TSPs; orf141, orf142, orf143) were identified with high confidence. All TSPs were predicted to encode putative depolymerase activities, including two endoglycosidases and one endorhamnosidase. The genome has been deposited in GenBank under the accession number PP_994976.1.
참고문헌 (9)
- Lamas A, "Salmonella spp.-A Global Challenge", pp.230~254 (2021), IntechOpen
- Hughes KA, "Biofilm susceptibility to bacteriophage attack : the role of phage-borne polysaccharide depolymerase", Microbiology, 144, pp.3039~3047 (1998)
- Chang C, "Bacteriophage-mediated control of biofilm : A promising new dawn for the future", Front. Microbiol, 13, pp.82858~ (2022)
- Kim S-H, "Isolation, characterization, and application of a novel, lytic phage vB_SalA_KFSST3 with depolymerase for the control of Salmonella and its biofilm on cantaloupe under cold temperature", Food Res. Int, 172, pp.113062~ (2023)
- Aziz RK, "The RAST Server : rapid annotations using subsystems technology", BMC Genomics, 9, pp.1~15 (2008)
- Zimmermann L, "A completely reimplemented MPI bioinformatics toolkit with a new HHpred server at its core", J. Mol. Biol, 430, pp.2237~2243 (2018)
- Sørensen AN, "Subtypes of tail spike proteins predicts the host range of Ackermannviridae phages", Comput. Sruct. Biotechnol. J, 19, pp.4854~4867 (2021)
- Wang S, "Strategy to combat biofilms : a focus on biofilm dispersal enzymes", NPJ Biofilms Microbiomes, 9, pp.63~ (2023)
- Perdones Á, "Properties of film-forming dispersions and films based on chitosan containing basil or thyme essential oil", Food Hydrocoll, 57, pp.271~279 (2016)