Isolation and Characterization of a Lytic and Highly Specific Phage against Yersinia enterocolitica as a Novel Biocontrol Agent
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
The aim of this study was to isolate and characterize a lytic Yersinia enterocolitica-specific phage (KFS-YE) as a biocontrol agent. KFS-YE was isolated and purified with the final concentration of (11.72 ± 0.03) log PFU/ml from poultry. As observed by transmission electron microscopy, KFS-YE consisted of an icosahedral head and a contractile tail, and was classified in the Myoviridae family. KFS-YE showed excellent narrow specificity against Y. enterocolitica only. Its lytic activity was stable at wide ranges of pH (4–11) and temperature (4–50°C). The latent period and burst size of KFS-YE were determined to be 45 min and 38 PFU/cell, respectively. KFS-YE showed relatively robust storage stability at -20, 4, and 22°C for 40 weeks. KFS-YE demonstrated a bactericidal effect in vitro against Y. enterocolitica and provided excellent efficiency with a multiplicity of infection as low as 0.01. This study demonstrated the excellent specificity, stability, and efficacy of KFS-YE as a novel biocontrol agent. KFS-YE may be employed as a practical and promising biocontrol agent against Y. enterocolitica in food.
Abstract
The aim of this study was to isolate and characterize a lytic Yersinia enterocolitica-specific phage (KFS-YE) as a biocontrol agent. KFS-YE was isolated and purified with the final concentration of (11.72 ± 0.03) log PFU/ml from poultry. As observed by transmission electron microscopy, KFS-YE consisted of an icosahedral head and a contractile tail, and was classified in the Myoviridae family. KFS-YE showed excellent narrow specificity against Y. enterocolitica only. Its lytic activity was stable at wide ranges of pH (4–11) and temperature (4–50°C). The latent period and burst size of KFS-YE were determined to be 45 min and 38 PFU/cell, respectively. KFS-YE showed relatively robust storage stability at -20, 4, and 22°C for 40 weeks. KFS-YE demonstrated a bactericidal effect in vitro against Y. enterocolitica and provided excellent efficiency with a multiplicity of infection as low as 0.01. This study demonstrated the excellent specificity, stability, and efficacy of KFS-YE as a novel biocontrol agent. KFS-YE may be employed as a practical and promising biocontrol agent against Y. enterocolitica in food.
참고문헌 (41)
- Pajunen M, "Bacteriophage ϕYeO3-12, specific for Yersinia enterocolitica serotype O: 3, is related to coliphages T3 and T7", J. Bacteriol, 182, pp.5114~5120 (2000)
- Salem M, "Isolation and characterization of Yersinia-specific bacteriophages from pig stools in Finland", J. Appl. Microbiol, 118, pp.599~608 (2015)
- Rahman A, "Yersinia enterocolitica: Epidemiological studies and outbreaks", J. Pathog, 211, pp.239391~ (2011)
- Harris L, "Outbreaks associated with fresh produce:incidence, growth, and survival of pathogens in fresh and fresh-cut produce", Compr. Rev. Food Sci. Food Saf, 2, pp.78~141 (2003)
- MacDonald E, "National outbreak of Yersinia enterocolitica infections in military and civilian populations associated with consumption of mixed salad, Norway, 2014", Euro Surveill, 21 (2016)[DOI]
- EFSA, "The european union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2010", Euro Surveill, 17, pp.2597~ (2012)
- Bottone EJ., "Yersinia enterocolitica: the charisma continues", Clin. Microbiol. Rev, 10, pp.257~276 (1997)
- Rashid MH, "A Yersinia pestis-specific, lytic phage preparation significantly reduces viable Y. pestis on various hard surfaces experimentally contaminated with the bacterium", Bacteriophage, 2, pp.168~177 (2012)
- Hwang S, "Isolation and characterization of bacteriophages specific for Campylobacter jejuni", Microbiol Immunol, 53, pp.559~566 (2009)
- Park M-K, "The effect of incubation temperature on the binding of Salmonella typhimurium to phage-based magnetoelastic biosensors", Sens. Actuators B:Chem, 160, pp.1427~1433 (2011)
- Byeon HM, "Lytic phage-based magnetoelastic biosensors for onsite detection of methicillin-resistant Staphylococcus aureus on spinach leaves", J. Electrochem. Soc, 162, pp.B230~B235 (2015)
- Hudson J, "Bacteriophages as biocontrol agents in food", J. Food Prot, 68, pp.426~437 (2005)
- Owens J, "The isolation and characterization of Campylobacter jejuni bacteriophages from free range and indoor poultry", Vet. Microbiol, 162, pp.144~150 (2013)
- 최인영, "Isolation and Characterization of a Novel Broad-host-range Bacteriophage Infecting Salmonella enterica subsp. enterica for Biocontrol and Rapid Detection", Journal of Microbiology and Biotechnology, 27(12), pp.2151~2155 (2017), 한국미생물·생명공학회
- Sharma M., "Lytic bacteriophages", Bacteriophage, 3, pp.e25518~ (2013)
- Cox N, "Salmonella penetration of egg shells and proliferation in broiler hatching eggs--a review", Poult. Sci, 79, pp.1571~1574 (2000)
- 오준현, "Recent Trends in Salmonella Outbreaks and Emerging Technology for Biocontrol of Salmonella Using Phages in Foods: A Review", Journal of Microbiology and Biotechnology, 27(12), pp.2075~2088 (2017), 한국미생물·생명공학회
- Stevenson R, "Isolation of Yersinia ruckeri bacteriophages", Appl. Environ. Microbiol, 47, pp.1201~1205 (1984)
- Popp A, "Comparative study of temperate bacteriophages isolated from Yersinia", Syst. Appl. Microbiol, 23, pp.469~478 (2000)
- Pujato SA, "Leuconostoc bacteriophages from blue cheese manufacture: long-term survival, resistance to thermal treatments, high pressure homogenization and chemical biocides of industrial application", Int. J. Food Microbiol, 177, pp.81~88 (2014)
- Park M-K, "The effect of incubation time for Salmonella Typhimurium binding to phage-based magnetoelastic biosensors", Food Control, 26, pp.539~545 (2012)
- Verma V, "Characterization of a T7-like lytic bacteriophage of Klebsiella pneumoniae B5055: a potential therapeutic agent", Curr. Microbiol, 59, pp.274~281 (2009)
- Ackermann H-W, "The species concept and its application to tailed phages", Arch. Virol, 124, pp.69~82 (1992)
- Bradley DE., "Ultrastructure of bacteriophage and bacteriocins", Bacteriol. Rev, 31, pp.230~314 (1967)
- Puapermpoonsiri U, "Stabilization of bacteriophage during freeze drying", Int. J. Pharm, 389, pp.168~175 (2010)
- Golec P, "A reliable method for storage of tailed phages", J. Microbiol. Methods, 84, pp.486~489 (2011)
- Li L, "Isolation and characterization of a virulent bacteriophage SPW specific for Staphylococcus aureus isolated from bovine mastitis of lactating dairy cattle", Mol. Biol. Rep, 41, pp.5829~5838 (2014)
- Haq IU, "Isolation and partial characterization of a virulent bacteriophage IHQ1 specific for Aeromonas punctata from stream water", Microb. Ecol, 63, pp.954~963 (2012)
- Park M, "Characterization and comparative genomic analysis of a novel bacteriophage SFP10 simultaneously inhibiting both Salmonella and Escherichia coli O157: H7", Appl. Environ. Microbiol, 78, pp.58~69 (2012)
- Yang H, "Isolation and characterization of a virulent bacteriophage AB1 of Acinetobacter baumannii", BMC Microbiol, 10, pp.131~ (2010)
- Ackermann H-W, "Long-term bacteriophage preservation", WFCC Newslett, 38, pp.35~40 (2004)
- Capra M, "Characterization of a new virulent phage (MLC-A) of Lactobacillus paracasei", J. Dairy Sci, 89, pp.2414~2423 (2006)
- Weinbauer MG., "Ecology of prokaryotic viruses", FEMS Microbiol. Rev, 28, pp.127~181 (2004)
- Jun JW, "Isolation and comparative genomic analysis of T1-like Shigella bacteriophage pSf-2", Curr. Microbiol, 72, pp.235~241 (2016)
- Shen G-H, "Isolation and characterization of ϕkm18p, a novel lytic phage with therapeutic potential against extensively drug resistant Acinetobacter baumannii", PLoS One, 7, pp.e46537~ (2012)
- Kim M, "Characterization of a T5-like coliphage SPC35 and differential development of resistance to SPC35in Salmonella Typhimurium and Escherichia coli", Appl. Environ. Microbiol, 77, pp.2041~2050 (2011)
- Yamaki S, "Characterization of a novel Morganella morganii bacteriophage FSP1 isolated from river water", FEMS Microbiol. Lett, 359, pp.166~172 (2014)
- GOPALSAMYGNANASEKARAN, "Genomic Insights and Its Comparative Analysis with Yersinia enterocolitica Reveals the Potential Virulence Determinants and Further Pathogenicity for Foodborne Outbreaks", Journal of Microbiology and Biotechnology, 27(2), pp.262~270 (2017), 한국미생물·생명공학회[DOI]
- Kasatiya S, "Morphology of Yersinia enterocolitica phages", Ann. Inst. Pasteur. Virol, 137, pp.59~69 (1986)
- Schwudke D, "Broad-host-range Yersinia phage PY100: genome sequence, proteome analysis of virions, and DNA packaging strategy", J. Bacteriol, 190, pp.332~342 (2008)
- Leon-Velarde CG, "Complete genome sequence of bacteriophage vB_YenP_AP5 which infects Yersinia enterocolitica of serotype O: 3", Virol. J, 11, pp.188~ (2014)