본문 바로가기
KERD
논문KCI 등재

Virulence Factors and Stability of Coliphages Specific to Escherichia coli O157:H7 and to Various E. coli Infection

Jong-Hyun Park|Eun Jin Kim|Hyun Joo Chang|Soojin Kwak
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2016|Journal of Microbiology and Biotechnology|제26권 제12호|pp. 2060-2065|DOI 10.4014/jmb.1609.09039

2

조회

0

다운로드

4

인용

0

참고문헌

키워드

stabilitybacteriophagevirulence geneE.coli O157:H7host spectrum
주제분류생물학

초록

Characteristics of E. coli O157:H7-specific infection bacteriophages (O157 coliphages) and broad-host-range bacteriophages for other E. coli serotypes (broad-host coliphages) were compared. The burst sizes of the two groups ranged from 40 to 176 PFU/infected cell. Distributions of the virulence factors stx1, stx2, ehxA, and saa between the two groups were not differentiated. Broad-host-range coliphages showed lower stability at 70oC, in relation to O157 coliphages. However, O157 coliphages showed high acid and ethanol tolerance by reduction of only 22% and 11% phages, respectively, under pH 3 and 70% ethanol for 1 h exposure. Therefore, these results revealed that the O157 coliphages might be more stable under harsh environments, which might explain their effective infection of the acid-tolerant E. coli O157:H7.

Abstract

Characteristics of E. coli O157:H7-specific infection bacteriophages (O157 coliphages) and broad-host-range bacteriophages for other E. coli serotypes (broad-host coliphages) were compared. The burst sizes of the two groups ranged from 40 to 176 PFU/infected cell. Distributions of the virulence factors stx1, stx2, ehxA, and saa between the two groups were not differentiated. Broad-host-range coliphages showed lower stability at 70oC, in relation to O157 coliphages. However, O157 coliphages showed high acid and ethanol tolerance by reduction of only 22% and 11% phages, respectively, under pH 3 and 70% ethanol for 1 h exposure. Therefore, these results revealed that the O157 coliphages might be more stable under harsh environments, which might explain their effective infection of the acid-tolerant E. coli O157:H7.

참고문헌 (28)

  1. Abdul-Raouf UM, "Survival and growth of Escherichia coli O157:H7 in g round, roasted beef as affected by pH, acidulants, and temperature", Appl. Environ. Microbiol, 59, pp.2364~2368 (1993)
  2. Amisano G, "Diarrheagenic Escherichia coli in acute gastroenteritis in infants in North-West Italy", New Microbiol, 34, pp.45~51 (2011)
  3. Bergan J, "Shiga toxins", Toxicon, 60, pp.1085~1107 (2012)
  4. Beutin L, "Isolation of enterohemolysin (Ehly2)-associated sequences encoded on temperate phages of Escherichia coli", Gene, 132, pp.95~99 (1993)
  5. Boyd EF, "Common themes among bacteriophage-encoded virulence factors and diversity among the bacteriophages involved", Trends Microbiol, 10, pp.521~529 (2002)
  6. Gerrish RS, "PCR versus hybridization for detecting virulence genes of enterohemorrhagic Escherichia coli", Emerg. Infect. Dis, 13, pp.1253~1258 (2007)
  7. Gould LH, "Increased recognition of non-O157 Shiga toxin–producing Escherichia coli infections in the United States during 2000–2010: epidemiologic features and comparison with E. coli O157 infections", Foodborne Pathog. Dis, 10, pp.453~460 (2013)
  8. Hedican EB, "Characteristics of O157 versus non-O157Shiga toxin-producing Escherichia coli infections in Minnesota, 2000–2006", Clin. Infect. Dis, 49, pp.358~364 (2006)
  9. Huang A, "Characterization of a bacteriophage that carries the genes for production of Shigalike toxin 1 in Escherichia coli", J. Bacteriol, 169, pp.4308~4312 (1987)
  10. Jamal M, "Characterization of new Myoviridae bacteriophage WZ1against multi-drug resistant (MDR) Shigella dysenteriae", J. Basic Microbiol, 55, pp.420~431 (2015)
  11. Kim EJ, "Characterization and infection receptor analysis of bacteriophages for Escherichia coli O157:H7 and non-O157Shiga toxin-producing E. coli", Master, pp.2015
  12. 김은진, "Morphological features and lipopolysaccharide attachment of coliphages specific to Escherichia coli O157:H7 and to a broad range of E. coli hosts", Applied Biological Chemistry, 59(1), pp.109~116 (2016), 한국응용생명화학회
  13. Koodi L, "Acid tolerance of Escherichia coli O157 : H7 and its survival in apple juice", Microbios, 104, pp.167~175 (2001)
  14. Lacey RW, "Mechanisms of resistance to beta-lactam antibiotics in Staphylococcus aureus", Scand. J. Infect. Dis, 42, pp.64~71 (1984)
  15. Law D, "Virulence factors of Escherichia coli O157 and other Shiga toxin-producing E. coli", J. Appl. Microbiol, 88, pp.729~745 (2000)
  16. Lee YD, "Characteristics of coliphage ECP4 and potential use as a sanitizing agent for biocontrol of Escherichia coli O157 : H7", Food Control, 34, pp.255~260 (2013)
  17. Li S, "Characterization and genome sequencing of a novel coliphage isolated from engineered Escherichia coli", Intervirology, 53, pp.211~220 (2010)
  18. Nataro JP, "Diarrheagenic Escherichia coli", Clin. Microbiol. Rev, 11, pp.142~201 (1998)
  19. O’Brien AD, "Shiga-like toxin-converting phages from Escherichia coli strains that cause hemorrhagic colitis or infantile diarrhea", Science, 226, pp.694~696 (1984)
  20. Park M, "Characterization and comparative genomic analysis of a novel bacteriophage, SFP10, simultaneously inhibiting both Salmonella enterica and Escherichia coli O157:H7", Appl. Environ. Microbiol, 78, pp.58~69 (2012)
  21. Paton AW, "Direct detection and characterization of Shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA, and saa", J. Clin. Microbiol, 40, pp.271~274 (2002)
  22. Paton AW, "Characterization of Saa, a novel autoagglutinating adhesin produced by locus of enterocyte effacement-negative Shigatoxigenic Escherichia coli strains that are virulent for humans", Infect. Immun, 69, pp.6999~7009 (2001)
  23. Rode TM, "High stability of Stx2 phage in food and under food-processing conditions", Appl. Environ. Microbiol, 77, pp.5336~5341 (2011)
  24. Sambrook J, "Molecular Cloning: A Laboratory Manual, Vol. 999" (2001), Cold Spring Harbor Laboratory Press
  25. Sánchez S, "Detection and characterisation of Shiga toxin-producing Escherichia coli other than Escherichia coli O157: H7 in wild ruminants", Vet. J, 180, pp.384~388 (2009)
  26. Schmidt H, "Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157:H7strain EDL 933", Infect. Immun, 63, pp.1055~1061 (1995)
  27. Skippington E, "Lateral genetic transfer and the construction of genetic exchange communities", FEMS Microbiol. Rev, 35, pp.707~735 (2011)
  28. Wagner PL, "Bacteriophage control of bacterial virulence", Infect. Immun, 70, pp.3985~3993 (2002)

관련 논문AI추천

Isolation and Characterization of a Novel Broad-host-range Bacteriophage Infecting Salmonella enterica subsp. enterica for Biocontrol and Rapid Detection김혜진, 박미경, 이주훈, 최인영한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2017 · 제27권 제12호Characterization of a lytic phage KFS-EC3 infecting multiple foodborne pathogens김수현, 박미경, 박예림, 정혜주한국식품저장유통학회 · Food Science and Preservation2022 · 제29권 제7호Isolation and Characterization of a Lytic and Highly Specific Phage against Yersinia enterocolitica as a Novel Biocontrol Agent곽경민, 박미경, 오준현, 이진영, 최인영한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2018 · 제28권 제11호Isolation and characterization of a lytic Salmonella Typhimurium-specific phage as a potential biofilm control agent김수현, 박미경한국식품저장유통학회 · Food Science and Preservation2023 · 제30권 제1호도축장으로부터 분리된 Shigella sonnei 특이적 박테리오파지의 특성 연구곽경민, 김우주, 김형우, 명진종, 박미경, 서정욱, 이민정, 장진욱한국식품저장유통학회 · Food Science and Preservation2018 · 제25권 제3호Characterization of Diarrheagenic Escherichia coli Isolated from Fresh Beef, Pork, and Chicken Meat in Korean Markets구민선, 장혜진, 조용선한국미생물·생명공학회 · 한국미생물·생명공학회지2020 · 제48권 제2호

이 간행물의 다른 논문 (13건)

Isolation and Proteomic Analysis of a Chlamydomonas reinhardtii Mutant with Enhanced Lipid Production by the Gamma Irradiation Method박현, 임상용, Jaewon Baek, Jong-Il Choi, Si Jae Parkpp. 2066-2075Improved NADPH Regeneration for Fungal Cytochrome P450 Monooxygenase by Co-Expressing Bacterial Glucose Dehydrogenase in Resting-Cell Biotransformation of Recombinant YeastMd Murshidul Ahsan, 윤형돈, Dowoo Lee, Pradeepraj Durairaj, Hyunwoo Jeonpp. 2076-2086Cloning, Expression, and Characterization of a Cold-Adapted Shikimate Kinase from the Psychrophilic Bacterium Colwellia psychrerythraea 34HYoungBaekHur, SOO WAN NAM, 김학준, Wahyu Sri Kunto Nugroho, Dong-Woo Kim, Jong-Cheol Hanpp. 2087-2097Production of Methanol from Methane by Encapsulated Methylosinus sporiumIn-Won Kim, Liaoyuan Zhang, Sanjay K. S. Patel, Sanjeet Mehariya, Sachin V. Otari, Bharat Madan, Jung Rim Haw, Jung-Kul Lee, Jae-Hoon Jeongpp. 2098-2105Metagenomic and Proteomic Analyses of a Mangrove Microbial Community Following Green Macroalgae Enteromorpha prolifera DegradationHetong Lin, Lingwei Ruan, Chao Zhao, Yijing Wu, Bin Liu, Zheng Xiaopp. 2127-2137
원문 보기