남.서해안과 동중국해 자연산 어류에서 Red Sea Bream Iridovirus (RSIV)의 검출
Detection of Red Sea Bream Iridovirus (RSIV) from marine fish in the Southern Coastal Area and East China Sea
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참고문헌
초록
Red sea bream iridovius (RSIV)는 우리나라와 일본의 참돔 (Pagrus major), 돌돔(Oplegnathus fasciatus) 등 주로 돔류에 질병을 유발하는 어류 병원바이러스로 알려져 왔으나, 돔류 이외에도 감수성을 나타내는 어종이 매우 다양하며 아시아를 중심으로 고수온기에 대량 폐사를 유발하는 중요한 원인체로 보고되고 있다. 본 연구는 RSIV에 대한 자연산어류에서 검출동향의 모니터링을 통해 금후 red sea bream iridoviral disease (RSIVD)에 대한 예방학적 접근의 일환으로서 자연산 어류로부터의 RSIV 검출 및 지리적 분포, 보균 어종에 대한 유전학적인 조사를 실시하였다. 조사는 2003년과, 2005년도에 동중국해역 3지점, 서해안 5지점 그리고 남해안 5지점에서 어류를 채집하여 PCR을 이용한 검출결과, 자연산 해산어류 160마리 중 39마리에서 RSIV가 검출되어 24.3%의 높은 검출율을 보였으며 특히 조사된 어류 중 상어목에서 가장 높은 검출율을 보였다. 자연산 어류에서 분리한 RSIV 분리주는 양식산어류에서 분리된 RSIV-K strain과 염기서열 분석에서 97.2%～100%, 아미노산 분석에서 94.7～100% 유사성을 나타내었으며 megalocytivirus Subgroup Ⅲ에 속함을 확인하였다.
Abstract
Red sea bream iridovirus disease (RSIVD) cause massive economic losses in marine aquaculture industry in Korea. The causative agent of this disease (RSIV) infects a wide range of fish species. The aims of this study were to monitor RSIV in wild marine fishes and to give critical information for controling the disease through prophylactic methods. Prevalence of the viral disease, geological distribution and reservoir of the virus were investigated using wild marine fishes captured in southern coast and east china sea for two years. (Polymerase Chain Reaction) PCR results showed that RSIV were detected in 39 (24.3%) out of 160 fish. MCP gene sequences of viral strains isolated in this study were closely related to that of a reference strain, red seabream-K, belonging to Megalocytivirus subgroup Ⅲ. The results suggest that some of wild marine fishes are RSIV carriers and may spread the pathogen directly to fish farmed in coastal area.
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