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Iridovirus의 해산 양식어류에 대한 병원성과 사육수에서의 검출

Pathogenicity of Iridovirus against Marine Fish and Its Detection in Culturing Seawater

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참고문헌

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주제분류수산학

초록

The susceptibility of five different marine fish to iridovirus IVS-1 infection was analyzed and found a higher the cumulative mortality in the order of rock bream (Oplegnathus fasciatus), red sea bream (Pagrus major), sea perch (Lateolabrax sp.), rockfish (Sebastes schlegeli) and black porgy (Acanthopagrus schlegeli). However, the concentrations of virus in the infected spleens of these species did not differ significantly. To determine the release of iridovirus from infected fish into culturing seawater, rock bream were challenged with iridovirus IVS-1 and the concentration of virus in the water was analyzed using PCR. Over the 10 days of the analysis, the linear relationship between the number of dead fish and viral DNA concentration found in culturing seawater should be considered direct evidence of horizontal iridovirus transmission.

Abstract

The susceptibility of five different marine fish to iridovirus IVS-1 infection was analyzed and found a higher the cumulative mortality in the order of rock bream (Oplegnathus fasciatus), red sea bream (Pagrus major), sea perch (Lateolabrax sp.), rockfish (Sebastes schlegeli) and black porgy (Acanthopagrus schlegeli). However, the concentrations of virus in the infected spleens of these species did not differ significantly. To determine the release of iridovirus from infected fish into culturing seawater, rock bream were challenged with iridovirus IVS-1 and the concentration of virus in the water was analyzed using PCR. Over the 10 days of the analysis, the linear relationship between the number of dead fish and viral DNA concentration found in culturing seawater should be considered direct evidence of horizontal iridovirus transmission.

참고문헌 (24)

  1. Chinchar, G., "Family iridoviridae. In: Virus Taxonomy Classification and Nomeclature of Viruses", pp.145~161 (2005), Academic Press, San Diego
  2. Do, J.W., "Complete genomic DNA sequence of rock bream iridovirus", Virology, 325, pp.351~363 (2004)
  3. Gibson-Kueh, S., "The pathology of systemic iridoviral disease in fish", J. Comp. Pathol, 111(119) (2003)
  4. He, J.G., "Systemic disease caused by an iridovirus-like agent in cultured mandarinfish, Siniperca chuatsi (Basilewsky), in China", J. Fish Dis, 23, pp.219~222 (2000)
  5. He, J.G., "Complete genome analysis of the mandarinfish infectious spleen and kidney necrosis iridovirus", Virology, 291, pp.126~139 (2001)
  6. He, J.G., "Experimental transmission, pathogenicity and phy- sical-chemical properties of infectious spleen and kidney necrosis virus (ISKNV)", Aquaculture, 204, pp.11~24 (2002)
  7. Inouye, K., "Iridovirus infection of cultured red sea bream", Pagrus major. Fish Pathol, 27, pp.19~27 (1992)
  8. Jeong, J.B., "Characterization of the DNA sequences in the genome of red sea bream iridoviruses isolated in Korea", Aquaculture, 220, pp.119~133 (2003)
  9. Jeong, J.B., "Asymptomatic iridovirus infection in various marine fishes detected by a 2-step PCR method", Aquaculture, 255, pp.30~38 (2006)
  10. Kawakami, H, "Cultured fish species affected by red sea bream iridoviral disease from 1996 to 2000", Fish Pathol, 37, pp.45~47 (2000)
  11. Kitamura, S.I, "Occurrence of marine birnavirus through the year in coastal seawater in the Uwa Sea", Mar. Biotechnol, 2, pp.188~194 (2000)
  12. Lu, L., "Complete genome sequence analysis of an iridovirus isolated from the orange-spotted grouper, Epinephelus coioides", Virology, 339, pp.81~100 (2005)
  13. Mahardika, K., "Susceptibility of juvenile humpback grouper, Cromileptes altivelis, to grouper sleepy disease iridovirus (GSDIV)", Dis. Aqua. Org, 59, pp.1~9 (2004)
  14. Matsuoka, S., "Cultured fish species affected by red sea bream iridoviral disease from 1991 to 1995", Fish Pathol, 31, pp.233~234 (1995)
  15. Nakajima, K, "Biological and physico-chemical properties of the iridovirus isolated from cultured red sea bream, Pagrus major", Fish Pathol, 29, pp.29~33 (1994)
  16. Nakajima, K., "Immunofluorescence test for the rapid diagnosis of red sea bream iridovirus infection using monoclonal antibody", Fish Pathol, 30, pp.115~119 (1995)
  17. Oshima, S., "A method for direct DNA am- plification of uncharacterized DNA viruses and for development of a viral polymerase chain reaction assay: application to the red sea bream iridovirus", Anal. Biochem, 242, pp.15~19 (1996)
  18. Rodger, H.D., "Systemic iridovirus infection in freshwater angelfish, Pterophyllum scalare (Liechtenstein)", J. Fish Dis, 20, pp.69~72 (1997)
  19. Sano, M., "Susceptibility of fish cultured in subtropical area of Japan to red sea bream iridovirus", Fish Pathol, 36, pp.38~39 (2001)
  20. Song, W.J., "Functional genomics analysis of Singapore grouper iridovirus: complete sequence determination and proteomic analysis", J. Virol, 78, pp.12576~12590 (2004)
  21. Sudthongkong, C., "Viral DNA sequences of genes encoding the ATPase and the major capsid protein of tropical iridovirus isolates which are pathogenic to fishes in Japan, South China Sea and Southeast Asian countries", Arch. Virol, pp.2089~2109 (2002)
  22. Tamai, T., "Development of DNA diagnostic methods for the detection of new fish iridoviral disease", Cytotechnology, 23, pp.211~220 (1997)
  23. Tidona, C.A, "The complete DNA sequence of lymphocystic disease virus.Virology,230,207-216", Virology, 230, pp.207~216 (1997)
  24. Tsai, C.T., "Complete genome sequence of the grouper iridovirus and comparison of genomic organization with those of other iridoviruses.J.Virol.,79,2010-2023", J. Virol, 79, pp.2010~2023 (2005)

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