틸라피아의 혈액성상에 미치는 온도와 비소의 복합적 영향
Combined effects of temperature and arsenic on hematological parameters of tilapia Oreochromis niloticus
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
본 연구는 어류의 온도변화 (20, 25 및 30℃)에 따른생리적 변동에 미치는 비소 (200 및 400 ppb)의 영향을파악하기 위하여 틸라피아, Oreochromis niloticus를대상으로 이들의 혈액학적 변동을 검토하였다. 틸라피아의 적혈구 수는 온도 30℃의 200 ppb 이상,Ht는 온도 25℃ 및 30℃의 400 ppb, Hb 농도는 온도30℃의 200 ppb 이상에서 유의한 증를 나타냈다. 혈청무기성분인 Ca농도는 수온 30℃의 400 ppb에서 유의하게 증가하였으나, Mg농도는 변동을 나타내지 않았다. 혈청 총 단백질농도는 온도 25 및 30℃의 비소농도400 ppb, glucose농도는 온도 25℃의 200 ppb 비소농도,온도 25 및 30℃의 400 ppb 비소농도에서 유의하게증가하였다. 혈청 GOT활성은 온도 25 및 30℃의 200ppb 이상의 비소농도, GPT활성은 온도 20, 25 및 30℃의 200 ppb 이상의 비소농도에서 유의한 증가를 나타냈다. 이상의 결과 및 논의에서 틸라피아의 혈액성상,혈청의 무기, 유기농도 및 효소활성은 비소농도 200ppb 이상에서 부분적으로 영향을 받고 있으며, 이 영향은 온도가 높을수록 영향이 클 것으로 생각된다.
Abstract
Experiments were carried out to determine the hematological changes in freshwater tilapia Oreochromis niloticus, after water temperature (20, 25 and 30℃) and arsenic concentrations (200, and 400 ppb) exposure 20 days. The RBC count and hematocrit (Ht) were significantly increased in As 400 ppb and water temperature 30℃. Hemoglobin (Hb) was significantly increased under combined As (200 and 400 ppb) and water temperature 30℃. The tilapia exposed to water temperature 30℃ and As concentration 400 ppb increase significantly in calcium concentration. The high concentrations of total protein and glucose concentrations were observed at water temperature 30℃ and As concentration 400 ppb. A significant increment GOT and GPT activities in the tilapia serum noticed at water temperature (25 and 30℃) and As concentration (200, 400 ppb). These results indicate that tilapia can be affected by high water temperature (30℃) and As concentration (400 ppb) in terms of inorganic matter, organic matter and enzyme activity in serum.
참고문헌 (32)
- Akter, K.F., "Arsenic speciation and toxicity in biological systems", Rev. Environ. Contam. Toxicol, 184, pp.97~149 (2005)
- Ayotte, J.D., "Arsenic in groundwater in eastern New England:accurrence, controls, and human health implications", Environ. Sci. Technol, 37, pp.2075~2083 (2003)
- Bhattacharya, A., "Induction of oxidative stress by As in Clarias batrachus: involvement of peroxisomes", Ecotoxicol. Evioron. Saf, 66, pp.178~187 (2007)
- Drastichova, J., "Effects of Cadmium on Hematological Indices of Common Carp(Cyprinus carpio L.)", Eviron. Com. Toxicol, 72, pp.725~732 (2004)
- Eisler, R., "Arsenic hazards to fish, wild life and invertrates: a synoptic review", U.S. Fish. Wildl. Serv. Biol, 85, pp.1~12 (1988)
- Federici, G., "Toxicity of titinium dioxide nanoparticles to rainbow trout(Oncorhynchus mykiss):gill injury, oxidative stress, and other physiological effects", Aqua. Toxicol, 84, pp.415~430 (2007)
- Figueiredo, A., "Effects of gender and temperature on oxidative stress enzymes in Nile tilapia Oreochromis niloticus exposed to paraquat", Biochem. Physiol, 85, pp.97~103 (2006)
- Hall, L.W Jr., "Effect of power plant coal pile and coal waste runoff and leachate on aquatic biota: an overview with research recommendations", Critic Reviews in Toxicol, 10, pp.287~302 (1982)
- Heather, B., "Arsenic exposure alters hepatic arsenic species composition and stress-mediated gene expression in the common killifish(Fundulus heteroclitus)", Aquat. Toxicol, 77, pp.257~266 (2005)
- Ishaque, A.B., "Developmental arrest in Japanese medaka(Oryzias latipes) embryos exposed to sublethal concentrations of atrazine and arsenic trioxide", J. Environ. Biol, 25, pp.1~6 (2004)
- Karagas, M.R., "Assessment of cancer risk and environmental levels of arsenic in New Hampshire", Environ. Health, 205, pp.85~94 (2002)
- Kobayashi, Y., "Stability of As metabolites As triglutathione (As(GS)3) and methyl As diglutathione (CH2As(GS)2), in rat bile", Toxicol, 211, pp.115~123 (2005)
- Larsen, E.H., "Arsenic concentrations correlate with salinity for fish taken from the North Sea and Baltic Waters", J. Mar. Biol, 83, pp.283~284 (2003)
- Larsson, A., "Biochemical and hematological effects of a titanium dioxide industrial effluent on fish", Bull. Environ. Contam. Toxicol, 25, pp.427~435 (1980)
- Lavanya, S., "Hematological, biochemical and ionoregulatory responses of Indian major carp Catla catla during chronic sublethal exposure to inorganic arsenic", Chem, 82, pp.977~985 (2011)
- Liao, C.N., "Acute toxicity and bioaccumulation of arsenic in tilapia(Oreochrmis mossambicus) fron an blackfoot disease area in Taiwan", Environ. Toxicol, 18, pp.252~259 (2003)
- Limuro, Y., "NFκB prevents apoptosis and liver dysfunction during liver regeneration", J. Clin. Invest, 101, pp.802~811 (1998)
- Li, Z.H., "Hepatic antioxidant status and hematological parameters in raibow trout, Oncorhynchus mykiss, after chronic exposure to carbamazepine", Chem. Biol. Int, 183, pp.98~104 (2010)
- Mattsson, K., "Effect of pulpmill effuents and restricted diet on growth and phygiology of rainbow trout(Oncorhynchus mykiss)", Exotoxicol. Environ. Saf, 49, pp.144~154 (2001)
- 강주찬, "Effect of waterborne benomyl on the hematological and antioxidant parameters of the Nile tilapia, Oreochromis niloticus", PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 92, pp.138~143
- Mukgerjee, A., "Arsenic contamination in groundwater:a global perspective with emphasis on the Asian scenario", Health. Popul. Nutr, 24, pp.142~163 (2006)
- Nemcsok, J., "Comparative studies on the sensitivity of different fish species to metal pollution", Acta Biol. Hung, 33, pp.23~27 (1982)
- Oliverira, C.A., "Hematological findings in neotropical fish Hoplias malabaricus exposed to subchronic and dietary doses of methylmercury, inorganic lead, and tributyltin chloride", Environ. Research, 101, pp.74~80 (2006)
- Phillips D.J.H., "The chemical forms of arsenic in aquatic organisms and their interrelationships. Arsenic in the Environment. PartⅠ. Cycling and Characteristics", 263~288 (1994)
- Roy, S., "Arsenic-induced histopathology and synthesis of stress proteins in liver and kidney of Channa punctatus", Ecotoxicol. Environ. Saf, 65, pp.218~229 (2006)
- Smet, S. M., "Stress Responses and Changes in Protein Metabolism in Carp Cyprinus carpio during Cadmium Exposure", Ecotoxicol. Environ. Saf, 48, pp.255~262 (2001)
- Suvetha, L., "Influence of cypermethrin toxicity on ionic regulation and gill Na+", K+-ATPase activity of a freshwater teleost fish Cyprinus Carpio, 2010, pp.29, 44~49
- Tripathi, S., "Effect of acute exposure of sodium arsenite (Na2AsO3) on some hematological parameters of Clarias batrachus (common Indian catfish) in vivo", Environ. Health, 45, pp.183~188 (2003)
- Waring, C.P., "The effects of handling on flounder(Platichthys flesus L.) and atlantic salmon(Salmo salar L.)", J. Fish. Biol, 41, pp.131~144 (1992)
- Wendelaar bonga, S.E., "The stress response in fish", Physiol, 77, pp.591~625 (1997)
- Wilson, R.W., "The physiological responses of freshwater rainbow trout, Oncorhinchus mykiss, during acutely lethal copper exposure", J. Comp. Physiol.B, 163, pp.38~47 (1993)
- Yang, J.L., "Serum metabolic enzyme activities and hepatocyte ultrastructure of common carp after gallium exposure", Zool. Stud, 42, pp.455~461 (2003)
