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틸라피아의 혈액성상에 미치는 온도와 비소의 복합적 영향

Combined effects of temperature and arsenic on hematological parameters of tilapia Oreochromis niloticus

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초록

본 연구는 어류의 온도변화 (20, 25 및 30℃)에 따른생리적 변동에 미치는 비소 (200 및 400 ppb)의 영향을파악하기 위하여 틸라피아, Oreochromis niloticus를대상으로 이들의 혈액학적 변동을 검토하였다. 틸라피아의 적혈구 수는 온도 30℃의 200 ppb 이상,Ht는 온도 25℃ 및 30℃의 400 ppb, Hb 농도는 온도30℃의 200 ppb 이상에서 유의한 증를 나타냈다. 혈청무기성분인 Ca농도는 수온 30℃의 400 ppb에서 유의하게 증가하였으나, Mg농도는 변동을 나타내지 않았다. 혈청 총 단백질농도는 온도 25 및 30℃의 비소농도400 ppb, glucose농도는 온도 25℃의 200 ppb 비소농도,온도 25 및 30℃의 400 ppb 비소농도에서 유의하게증가하였다. 혈청 GOT활성은 온도 25 및 30℃의 200ppb 이상의 비소농도, GPT활성은 온도 20, 25 및 30℃의 200 ppb 이상의 비소농도에서 유의한 증가를 나타냈다. 이상의 결과 및 논의에서 틸라피아의 혈액성상,혈청의 무기, 유기농도 및 효소활성은 비소농도 200ppb 이상에서 부분적으로 영향을 받고 있으며, 이 영향은 온도가 높을수록 영향이 클 것으로 생각된다.

Abstract

Experiments were carried out to determine the hematological changes in freshwater tilapia Oreochromis niloticus, after water temperature (20, 25 and 30℃) and arsenic concentrations (200, and 400 ppb) exposure 20 days. The RBC count and hematocrit (Ht) were significantly increased in As 400 ppb and water temperature 30℃. Hemoglobin (Hb) was significantly increased under combined As (200 and 400 ppb) and water temperature 30℃. The tilapia exposed to water temperature 30℃ and As concentration 400 ppb increase significantly in calcium concentration. The high concentrations of total protein and glucose concentrations were observed at water temperature 30℃ and As concentration 400 ppb. A significant increment GOT and GPT activities in the tilapia serum noticed at water temperature (25 and 30℃) and As concentration (200, 400 ppb). These results indicate that tilapia can be affected by high water temperature (30℃) and As concentration (400 ppb) in terms of inorganic matter, organic matter and enzyme activity in serum.

참고문헌 (32)

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