본문 바로가기
KERD
논문KCI 등재

Thermal Effects on Bioaccumulation and Antioxidant Enzyme Response in the Liver and Gills of Tilapia Oreochromis niloticus Exposed to Arsenic

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류수산학

초록

The effects of waterborne arsenic (As) exposure on bioaccumulation and antioxidant defenses were examined in the liver and gills of tilapia, Oreochromis niloticus, under thermal stress. Tilapia were exposed to different As concentrations (0, 200, and 400 μg L-1) at three water temperatures (20, 25, and 30°C) for 20 days. After As exposure, higher levels of As accumulation were observed in the gills compared with the liver in elevated water temperatures. In terms of the antioxidant response, glutathione (GSH) levels and the activities of glutathione reductase (GR), glutathione peroxidase (GPx), and glutathione S-transferase (GST) significantly decreased in the liver and gills of tilapia exposed to As for 20 days, regardless of the As concentration (200 and 400 μg L-1) or water temperature. These enzyme activities exhibited greater fluctuations in the liver and gills of tilapia after As exposure in water warmer than 20°C. The present findings suggest that the simultaneous stress of temperature change and As exposure can accelerate As accumulation and alter the antioxidant enzymes activities of tilapia.

Abstract

The effects of waterborne arsenic (As) exposure on bioaccumulation and antioxidant defenses were examined in the liver and gills of tilapia, Oreochromis niloticus, under thermal stress. Tilapia were exposed to different As concentrations (0, 200, and 400 μg L-1) at three water temperatures (20, 25, and 30°C) for 20 days. After As exposure, higher levels of As accumulation were observed in the gills compared with the liver in elevated water temperatures. In terms of the antioxidant response, glutathione (GSH) levels and the activities of glutathione reductase (GR), glutathione peroxidase (GPx), and glutathione S-transferase (GST) significantly decreased in the liver and gills of tilapia exposed to As for 20 days, regardless of the As concentration (200 and 400 μg L-1) or water temperature. These enzyme activities exhibited greater fluctuations in the liver and gills of tilapia after As exposure in water warmer than 20°C. The present findings suggest that the simultaneous stress of temperature change and As exposure can accelerate As accumulation and alter the antioxidant enzymes activities of tilapia.

참고문헌 (42)

  1. Ahmed MK, "Arsenic induced toxicity and histopathological changes in gill and liver tissues of freshwater fish, tilapia(Oreochromis mossambicus)", Exp Toxicol Pathol, 65, pp.903~909 (2013)
  2. An MI, "Activity of antioxidant enzymes and physiological responses in ark shell, Scapharca broughtonii, exposed to thermal and osmotic stress : effects on hemolyph and biochemical parameters", Comp Biochem Physiol B, 155, pp.34~42 (2010)
  3. Bajanyukova TV, "Oxidative stress and antioxidant defense responses by goldfish tissues to acute change of temperature from 3 to 23°C", J Therm Biol, pp.227~234 (2007)
  4. Bell JG, "Some effects of vitamin E and selenium deprivation on tissue enzyme levels and indices of tissue peroxidation in rainbow trout(Salmo gairdneri)", Br J Natr, 53, pp.149~157 (1985)
  5. Beutler E, "Red cell metabolism: Manual of biochemical methods" (1984), Grune Stratton, Ic
  6. Bocchetti R, "Trace metal concentrations and susceptibility to oxidative stress in the polychaete Sabella spallanzanii(Gmelin)(Sabellidae) : potential role of antioxidant in revealing stressful environmental conditions in the Mediterranean", Arch Environ Contam Toxicol, 46, pp.353~361 (2004)
  7. Campana O, "Effect of lead on ALAD activity, metallothionein levels, and lipid peroxidation in blood, kidney, and liver of the toadfish Halobatrachus didactylus", Ecotoxicol Environ Safety, 55, pp.116~125 (2003)
  8. Carvalho CS, "Effect of temperature on copper toxicity and hematological responses in the neotropical fish Prochilodus scrofa at low and high pH", Aquaculture, 251, pp.109~117 (2005)
  9. Chouchane S, "In Vitro Effect of Arsenical Compounds on Glutathione-Related Enzymes", Chem Res Toxicol, 14, pp.517~522 (2001)
  10. Cherkasov AS, "Combined effects of temperature acclimation and cadmium exposure on mitochondrial function in eastern oysters Crassostrea virginica gmelin(Bivalvia : Ostreidae)", Environ Toxicol Chem, 25, pp.2461~2469 (2006)
  11. Culioli JL, "Arsenic accumulation in a freshwater fish living in a contaminated river of Corsica, France", Ecotoxicol Environ Safe, 72, pp.1440~1445 (2009)
  12. Dopp E, "Organoarsenicals, uptake, metabolism, and toxicity", Met Ions Life Sci, 7, pp.231~265 (2010)
  13. Dringen R, "Glutathione metabolism in brain metabolic interaction between astrocytes and neurons in the defense against reactive oxygen species", Eur J Biochem, 267, pp.4912~4916 (2000)
  14. Ercal N, "Toxic metals and oxidative stress. Part 1. Mechanisms involved in metal-induced oxidative damage", Current Top Med Chem, 1, pp.529~539 (2001)
  15. Habig W, "Glutahione-S-transferases : The first enzymatic step in mercaptouric acid formation", J Biol Chem, 249, pp.7130~7139 (1974)
  16. Holwerda DA, "Cadmium kinetics in freshwater clams. V. Cadmium-copper interaction in metal accumulation by Anodonta cyngnea and characterization of metal binding protein", Arch Environ Contam Toxicol, 21, pp.432~437 (1991)
  17. Barry Halliwell, "Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?", British Journal of Pharmacology, 142(2), pp.231~255 (2004), Wiley-Blackwell[DOI]
  18. Hughes MF, "Oxidative stress, Disease and Cancer", pp.825~850 (2006), Imperial College Press
  19. Jezierska B, "The metal uptake and accumulation in fish living in polluted waters", Soil Water Poll Monitoring Protect Remed, 69, pp.107~114 (2006)
  20. Kapaj S, "Human health effects from chronic arsenic poisoning-A review", J Environ Sci Health Part A, 42, pp.2399~2428 (2006)
  21. Kaur M, "Heat stress-induced alterations of antioxidants in the freshwater fish Channa punctate Bloch", J Fish Biol, 67, pp.1653~1665 (2005)
  22. 김희연, "유통 중인 어류의 중금속 모니터링-비소, 카드뮴, 구리, 납, 망간, 아연, 총수은 -", 한국식품과학회지, 39(4), pp.353~359 (2007), 한국식품과학회
  23. Kock G, "Seasonal patterns of metal accumulation in Arctic char(salvelinus alpinus)form an oligotrophic Alpine lake related to temperature", Can J Fish Aquat Sci, 53, pp.780~786 (1996)
  24. Kotsanis N, "Arsenic induced liver hyperplasia and kidney fibrosis in rainbow trout(Oncorhynchus mykiss)by microinjection technique : A sensitive animal bioassay for environmental metal-toxicity", Bull Environ Contam Toxicol, 62, pp.169~178 (1999)
  25. Lantz RC, "Pulmonary biomarkers based on alterations in protein expression after exposure to arsenic", Environ Health Perspect, 115, pp.586~591 (2007)
  26. Liu SX, "Induction of oxyradicals by arsenic : implication for mechanism of gentoxicity", Proc Natl Acad Sci USA, 98, pp.1643~1648 (2001)
  27. McGeachy SM, "The impact of temperature on the acute toxicity of arsenate and arsenite to rainbow trout(Salmo gairdneri)", Ecotoxicol Environ Saf, 17, pp.86~93 (1989)
  28. Mishra D, "Reversal of arsenic-induced hepatic apotosis with combined administration of DMSA and its analogues in guinea pigs : role of glutathione and linked enzymes", Chem Res Toxicol, 21, pp.400~407 (2008)
  29. Pereira P, "The relevance of temporal and organ specific factors on metals accumulation and biochemical effects in feral fish(Liza aurata)under a moderate contamination scenario", Ecotoxicol Environ Saf, 73, pp.805~816 (2010)
  30. Powell ML, "Effect of temperature acclimation on metabolism and hemocyanin binding affinities in two crayfish, Procambarus clarkii and Procambarus zonangulus", Comp Biochem Physiol, 144, pp.211~217 (2006)
  31. Samuel S, "Protein oxidative damage in arsenic induced rat brain : influence of DL-alpha-lipoic acid", Toxicol Lett, 155, pp.27~34 (2005)
  32. Shi HL, "Oxidative mechanism of arsenic toxicity and carcinogenesis", Mol Cell Biochem, 255, pp.67~78 (2004)
  33. 성기탁, "한국 연근해 수온의 시공간적 장기변동 특성", 해양환경안전학회지, 16(4), pp.353~360 (2010), 해양환경안전학회
  34. Srivastava SK, "Glutathione metabolism of the erythrocyte", Biochem J, pp.353~357 (1970)
  35. Takatsu A, "Arsenic Accumulation in Organs of the Fresh Water Fish Tribolodon hakonensis", J Trace Elements Med Biol, 13, pp.176~179 (1999)
  36. Thomas DJ, "The cellular metabolism and systemic toxicity of Arsenic", Toxicol Appl Pharm, 176, pp.127~144 (2001)
  37. USEPA, "National primary drinking water regulations", Fed. Reg, 66, pp.6975~7066 (2001)
  38. Valko M, "Free-radicals, metals and antioxidants in oxidative stress-induced cancer", Chem Biol Interact, 160, pp.1~40 (2006)
  39. Ventura-Lima J, "Toxicological responses in Laeonereis acuta (Annelida, Polychaeta) after As exposure", Environ Int, 33, pp.559~564 (2007)
  40. Ventura-Lima J, "Effects of arsenic (As) exposure on the antioxidant status of gills of the zebrafish Danio rerio (Cyprinidae)", Comp Biochem Physiol Toxicol Pharmacol, 149, pp.538~543 (2009)
  41. Wang W, "Effects of temperature on growth, adenosine phosphates, ATPase and cellular defense response of juvenile shrimp, Macrobrachium nipponense", Aquaculture, 256, pp.624~630 (2006)
  42. Yu BP, "Cellular defenses against damage from reactive oxygen species", Physiol Rev, 74, pp.139~162 (1994)

관련 논문AI추천

이 간행물의 다른 논문 (14건)