국내 어류에서 분리된 Megalocytivirus의 유전형 분류 및 상관관계 분석
Genetic relatedness of Megalocytivirus from diseased fishes in Korea
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In this study, we collected 39 megalocytiviruses isolated from diseased fish in Korea from 2012 to 2018. Major capsid protein (MCP) gene, a part of vascular endothelial growth factor (VEGF) gene and histidine triad motif-like protein (HIT) genes of Megalocytivirus were targeted for PCR amplification and analysis of those DNA nucleotide sequences. Korean strains revealed two genotypes (red sea bream iridovirus and turbot reddish body iridovirus types) based on the phylogeny of MCP gene. The red sea bream iridovirus type (RSIV-type) megalocytiviruses were divided into RSIV- subgroup 1 and 2. From the phylogenetic analysis of the VEGF genes, a genotypic variant of RSIV-type Megalocytivirus was identified. The HIT-like protein gene was detected in RSIVs, but not in TBRIV and ISKNV, suggesting that HIT-like protein gene may be specific in RSIV.
Abstract
In this study, we collected 39 megalocytiviruses isolated from diseased fish in Korea from 2012 to 2018. Major capsid protein (MCP) gene, a part of vascular endothelial growth factor (VEGF) gene and histidine triad motif-like protein (HIT) genes of Megalocytivirus were targeted for PCR amplification and analysis of those DNA nucleotide sequences. Korean strains revealed two genotypes (red sea bream iridovirus and turbot reddish body iridovirus types) based on the phylogeny of MCP gene. The red sea bream iridovirus type (RSIV-type) megalocytiviruses were divided into RSIV- subgroup 1 and 2. From the phylogenetic analysis of the VEGF genes, a genotypic variant of RSIV-type Megalocytivirus was identified. The HIT-like protein gene was detected in RSIVs, but not in TBRIV and ISKNV, suggesting that HIT-like protein gene may be specific in RSIV.
참고문헌 (25)
- Brenner C, "Hint, Fhit and GalT : Function, Structure, Evolution and Mechanism of Three Branched of the Histidine Triad Superfamily of Nucleotide Hydrolases and Transferases", Biochemistry, 23, pp.9003~9014 (2002)
- Chinchar, V. G., "ICTV Virus Taxonomy Profile: Iridoviridae", J. Gen. Virol., 98, pp.890~891 (2017)
- J. W. Do, "Sequence variation in the gene encoding the major capsid protein of Korean fish iridoviruses", Archives of Virology, 150(2), pp.351~359 (2005), Springer Science and Business Media LLC[DOI]
- JW Do, "Phylogenetic analysis of the major capsid protein gene of iridovirus isolates from cultured flounders Paralichthys olivaceus in Korea", Diseases of Aquatic Organisms, 64, pp.193~200 (2005), Inter-Research Science Center[DOI]
- Eaton HE, "Comparative genomic analysis of the family Iridoviridae: re-annotating and defining the core set of iridovirus genes", Virology Journal, 4, pp.11~ (2007)
- Fu X, Li N., "Protective immunity against iridovirus disease in mandarin fish induced by recombinant major capsid protein of infectious spleen and kidney necrosis virus", Fish & Shellfish Immunology, 33, pp.880~885 (2012)
- Hall TA, "BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95", 98, Nucleic Acids Symposium Series, pp.1999, 41[DOI]
- Kiyoshi Inouye, "Iridovirus Infection of Cultured Red Sea Bream, Pagrus major.", Fish Pathology, 27(1), pp.19~27 (1992), The Japanese Society of Fish Pathology[DOI]
- S J Jung, "Iridovirus-like infection associated with high mortalities of striped beakperch, Oplegnathus fasciatus (Temminck et Schlegel), in southern coastal areas of the Korean peninsula", Journal of Fish Diseases, 23(3), pp.223~226 (2000), Wiley[DOI]
- K I Kim, "A natural infection by the red sea bream iridovirus-type Megalocytivirus in the golden mandarin fish Siniperca scherzeri", Journal of Fish Diseases, 41(8), pp.1229~1233 (2018), Wiley[DOI]
- Kim KI, "Genetic diversity of Megalocytivirus from cultured fish in Korea", Aquaculture, 509, pp.16~ (2019)[DOI]
- Jun Kurita, "Megalocytiviruses", Viruses, 4(4), pp.521~538 (2012), MDPI AG[DOI]
- 권세련, "Shift of Phylogenic Position in Megalocytiviruses Based on Three Different Genes", The Journal of Microbiology, 49(6), pp.981~986 (2011), 한국미생물학회
- 이월라, "남.서해안과 동중국해 자연산 어류에서 Red Sea Bream Iridovirus (RSIV)의 검출", 한국어병학회지, 20(3), pp.211~220 (2007), 한국어병학회
- Martin J, "Hit protein, mitochondria and cancer", Biochimica et Biophysica Acta, 1807, pp.626~632 (2011)
- A Mishra, "Comparative evaluation of MCP gene in worldwide strains of Megalocytivirus (Iridoviridae family) for early diagnostic marker", Journal of Fish Diseases, 41(1), pp.105~116 (2018), Wiley[DOI]
- "World Organization for Animal Health, Manual of Diagnostic Tests for Aquatic animal" (2018)[URL]
- Sohn SG, "Mass Mortalities of Cultured Striped Beakperch, Oplegnathus fasciatus by Irioviral Infection", Journal of Fish Pathology, 13, pp.121~127 (2000)
- 송준영, "Genetic Variation and Geographic Distribution of Megalocytiviruses", The Journal of Microbiology, 46(1), pp.29~33 (2008), 한국미생물학회
- R. Stohwasser, "Identification of the Gene Encoding the Major Capsid Protein of Insect Iridescent Virus Type 6 by Polymerase Chain Reaction", Journal of General Virology, 74(5), pp.873~879 (1993), Microbiology Society[DOI]
- Tidona, CA, "Is the major capsid protein of iridoviruses a suitable target for study of viral Evolution?", Virus Gene, 16, pp.59~66 (1998)
- Thompson JD, "CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice", Nucleic Acids Research, 22, pp.4673~4680 (1994)
- Tidona CA, "Is the Major Capsid Protein of Iridoviruses a Suitable Target for the Study of Viral Evolution?", Virus Genes, 16, pp.59~66 (1998)
- Z.-L. Wang, "Infectious Spleen and Kidney Necrosis Virus ORF48R Functions as a New Viral Vascular Endothelial Growth Factor", Journal of Virology, 82(9), pp.4371~4383 (2008), American Society for Microbiology[DOI]
- 원경미, "The First Report of a Megalocytivirus Infection in Farmed Starry Flounder, Platichthys stellatus, in Korea", Fisheries and Aquatic Sciences, 16(2), pp.93~99 (2013), 한국수산과학회